Label-free real-time detection of biotinylated bovine serum albumin using a low-cost optical cavity-based biosensor.

We have developed a low-cost optical cavity-based biosensor with a differential detection method for point-of-care medical diagnostics. To experimentally demonstrate its label-free real-time biosensing capability, we performed the detection of biotinylated bovine serum albumin (BSA). Streptavidin is introduced into the optical cavity structure and immobilized on 3-aminopropyltriethoxysilane (APTES) coated surface. After rinsing out unbound streptavidin with DI water, biotinylated BSA without any labeling is introduced. A CMOS camera captures the transmitted light of two different wavelengths passing through the optical cavity sensing area in real-time.
Then, the differential values are calculated to enhance the responsivity. We successfully demonstrated the label-free real-time detection of biotinylated BSA, and the measurement results matched well with the simulation results. The limit of detection of the optical cavity-based biosensor for the biotinylated BSA detection with the sensing area of 180 μm × 180 μm is estimated to be 2.82 pM, which could be reduced further for a smaller sensing area with the tradeoff of a longer sensing time.

Biotinylated Lipid-Coated NaLnF 4 Nanoparticles: Demonstrating the Use of Lanthanide Nanoparticle-Based Reporters in Suspension and Imaging Mass Cytometry

Lanthanide nanoparticles (LnNPs) have the potential to be used as high-sensitivity mass tag reporters in mass cytometry immunoassays. For this application, however, the LnNPs must be made colloidally stable in aqueous buffers, demonstrate minimal non-specific binding to cells, and have functional groups to attach antibodies or other targeting agents. One possible approach to address these requirements is by using lipid coating to modify the surface of the LnNPs. In this work, 39 nm diameter NaYF4:Yb, Er NPs (LnNPs) were coated with a lipid formulation consisting of egg sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-3-trimethylammonium propane, cholesterol-(polyethylene glycol-600), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000].
The resulting biotinylated lipid-coated LnNPs were characterized by dynamic light scattering to determine the hydrodynamic size and stability in phosphate buffered saline, and the composition of the lipid coatings was quantified by liquid chromatography-tandem mass spectrometry. The specific and non-specific binding of the biotinylated lipid-coated LnNPs to a model system of functionalized polystyrene microbeads were then tested by both suspension and imaging mass cytometry. We found that targeted binding with minimal non-specific binding can be achieved with the lipid-coated LnNPs and that the lipid composition of the coating has an impact on the performance of the LnNPs as mass cytometry reporters. These results additionally establish the importance of quantifying the composition of lipid-coated nanomaterials to optimize them more effectively for their desired application.

Synthesis of Biotinylated PAMAM G3 Dendrimers Substituted with R-Glycidol and Celecoxib/Simvastatin as Repurposed Drugs and Evaluation of Their Increased Additive Cytotoxicity for Cancer Cell Lines

Recent achievement in anticancer therapy considers the application of repurposed drugs in optimal combinations with the use of specific carriers for their targeted delivery. As a result, new optimized medications with reduced side effects can be obtained. In this study, two known anticancer drugs, celecoxib and/or simvastatin, were conjugated covalently with PAMAM G3 dendrimer and tested in vitro against human squamous carcinoma (SCC-15-15) and glioblastoma (U-118 MG) cells, as well as normal human fibroblasts (BJ). The obtained conjugates were also substituted with biotin and R-glycidol to increase their affinity for cancer cells and were characterized with NMR spectroscopy and dynamic light scattering technique.
Conjugates furnished with two celecoxib and four simvastatin residues revealed the very high effectiveness and dramatically decreased the SCC-15 and U-118 MG cell viability at very low concentrations with IC50 equal to about 3 µM. Its action was 20-50-fold stronger than that of either drug alone or as a mixture. Combined conjugate revealed also additive action since it was 2-8-fold more effective than conjugates with either single drug. The combined conjugate revealed rather low specificity since it was also highly cytotoxic for BJ cells. Despite this, it may be concluded that biotinylated and R-glycidylated PAMAM G3 dendrimers substituted with both celecoxib and simvastatin can be considered as a new perspective anticancer agent, effective in therapy of malignant, incurable glioblastomas.

Bovine Serum Albumin Biotinylated (Biotin-BSA)

BTN-BSA Alpha Diagnostics 5 mg 286 EUR

Bovine Serum Albumin (BSA) Monoclonal Antibody (Bovine), Biotinylated

4-MAA248Bo21-Biotin Cloud-Clone
  • 300.00 EUR
  • 2301.00 EUR
  • 687.00 EUR
  • 365.00 EUR
  • 214.00 EUR
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul

Bovine Serum Albumin

7-04585 CHI Scientific 10gr Ask for price

Bovine Serum Albumin

7-04586 CHI Scientific 50gr Ask for price

Bovine Serum Albumin

7-04587 CHI Scientific 100gr Ask for price

Bovine Serum Albumin

GE1877-250G Glentham Life Sciences 250 g 285 EUR

Bovine Serum Albumin

GE1877-25G Glentham Life Sciences 25 g 70 EUR

Bovine Serum Albumin

GE1877-500G Glentham Life Sciences 500 g 500 EUR

Bovine Serum Albumin

GE1877-100G Glentham Life Sciences 100 g 158 EUR

Bovine Serum Albumin

GE1877-10G Glentham Life Sciences 10 g 54 EUR

Bovine Serum Albumin

GE1877-50G Glentham Life Sciences 50 g 102 EUR

Bovine Serum Albumin

HY-D0842 MedChemExpress 10g 187 EUR

Bovine Serum Albumin (BSA) Monoclonal Antibody (General), Biotinylated

4-MAA248Ge21-Biotin Cloud-Clone
  • 300.00 EUR
  • 2301.00 EUR
  • 687.00 EUR
  • 365.00 EUR
  • 214.00 EUR
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul

Bovine Serum Albumin Standard

AR0191 BosterBio 20mL 70 EUR

Bovine Serum Albumin (BSA)

20-abx082611 Abbexa
  • 230.00 EUR
  • 112.00 EUR
  • 25 g
  • 5 g

Bovine Serum Albumin Antibody

20-abx133923 Abbexa
  • 356.00 EUR
  • 537.00 EUR
  • 217.00 EUR
  • 100 ul
  • 200 ul
  • 30 ul