Mycoplasma Detection Kits Applied Biological Materials

abm applied cells

**Applied Biological Materials (abm) offers services and/or reagents specific to COVID-19 research. Submit a quote request containing the word ‘COVID-19’ to receive more details.**

Applied Biological Materials (abm) provides high quality research reagents and custom services at incredible prices! We have an extensive range of products and custom services to make abm your one-stop shop for all of your research needs. Our lineup includes:

  • Mycoplasma Decontamination & Detection Services.
  • Cell Immortalization and Stable Cell Line Generation.
  • Lentivirus Production.
  • Adenovirus Production.
  • AAV Production.
  • CHO Cell Antibody Production.
  • Illumina-Certified CSPro Next Generation Sequencing.
  • ISO-Certified PCR/RT-PCR/qPCR Reagents.
  • siRNA/miRNA/CRISPR Vectors.
  • Viral Expression Vectors (Lentivirus, AAV, Adenovirus, HSV, Baculovirus).
  • Growth Factors.
  • HSV Production.
  • Baculovirus Production.
  • CRISPR Knock-Out/Knock-In Cell Lines
  • H&E and IHC Staining.

Making scientific discoveries shouldn’t cost you a fortune! Let abm be your primary research partner!

Please contact us for more information on any of our products and available services.

abm applied cells
abm applied cells

Description

Cryopreservation Medium by abm is a ready-to-use complete formulation suitable for the cryopreservation of various types of mammalian cells. With its unique formula this medium can be used to freeze cells cultured in classical media such as DMEM, DMEM/F12, RPMI 1640, and Ham’s F-12, as well as others.

This product is composed of 10% DMSO along with additional optimized components that will improve cell recovery after thawing. Every lot of Cryopreservation Medium by abm is tested for pH, sterility, and endotoxin.

Supplied by Applied Biological Materials (abm). Usually ships in 3 business days.

Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.

Mycoplasma Detection Kits – Conventional PCR

Mycoplasma contamination is a serious problem for laboratories due to its impact on cell properties and safety of biological products. Mycoplasma contaminations in cell cultures are unnoticeable (macroscopically as well as microscopically), making routine tests for reliable mycoplasma detection necessary. They represent a serious concern for academic and biopharmaceutical laboratories due to their effects on cell properties and on safety of biological products.
Mycoplasma (term often interchangeably used with Mollicutes, which include Mycoplasma, Acholeplasma, and Spiroplasma) are ubiquitous in the environment. In cell culture laboratories, 95 % of all mycoplasma infections in continuous cell lines are caused by as few as six contaminant species of bovine, swine, and human origin. These microbial agents easily spread from an infected cell culture batch to another by aerosol transmission or inadequate aseptic techniques.
Regular mycoplasma testing and control procedures help avoiding the costly consequences of a cell culture contamination and ensuring safe biopharmaceuticals and reliable scientific results. Consequently, most scientific publishers do not accept submission of cell-based studies without proof of the performed mycoplasma tests.
Compared to alternative methods, PCR-based mycoplasma detection kits are rapid, simple and do not require viable mycoplasma, special equipment, or extensive staff training.

Glutaraldehyde, Biological Grade, 50%

00377-1 1L
EUR 189
Description: 111-30-8

Glutaraldehyde, Biological Grade, 50%

00377-4 4x1 L
EUR 445
Description: 111-30-8

Glutaraldehyde, Biological Grade, 50%

00377-500 500ml
EUR 101
Description: 111-30-8

Spore Ampoule Biological Indicators

AUT2038 PK50
EUR 392.16

RNase R

E049 500 U (50 μl)
EUR 295
Description: RNase R is an E. coli exoribonuclease which exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven nucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.

Opti-Protein Ultra Marker

G623 500 μl/100 loads
EUR 85

BlasTaq Probe 2X qPCR MasterMix

G890 4 x 1.25 ml
EUR 135
Description: BlasTaq™ Probe 2X qPCR MasterMix is designed for TaqMan probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix (dNTPs, BlasTaq™ DNA polymerase, MgCl2, and other proprietary buffer components) have been developed for superb performance in sensitivity and signal-to-noise ratio. The MasterMix is suitable for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. ROX reference dye is provided separately, making it universally compatible with most qPCR instruments.

BlasTaq 2X qPCR MasterMix

G891 500 rxn (4 x 1.25 ml)
EUR 135
Description: BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.Key Features• Enable streamlined protocol in a simple reaction set-up. • Allow accurate quantification of a variety of gene targets. • Reduce pipetting steps to minimize the risk of contamination.• Compatible with most real-time PCR instruments.

BlasTaq 2X qPCR MasterMix

G892 2,500 rxn (25.0 ml)
EUR 545
Description: BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.Key Features• Enable streamlined protocol in a simple reaction set-up. • Allow accurate quantification of a variety of gene targets. • Reduce pipetting steps to minimize the risk of contamination.• Compatible with most real-time PCR instruments.

BlasTaq DNA Polymerase

G894 400 rxn (200 μl)
EUR 80
Description: BlasTaq™ DNA Polymerase is a strategically-engineered, next generation Taq Polymerasethat has rapid extension rates and robust performance. With specialized reactionconditions, this polymerase provides increased processivity, yields, and sensitivity, whileshortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonucleaseactivity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can beused with TA cloning vectors.

100bp Opti-DNA Marker

G016 500 μl/100 loads
EUR 45
Description: PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 17 bands, ranging from 50 bp – 1.5 kb, for molecular weight standards in agarose gel electrophoresis. The 200 and 500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

1kb Opti-DNA Marker

G106 500 μl/100 loads
EUR 45
Description: PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 19 bands, ranging from 100 pb – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

Safe-Green

G108 1.0 ml
EUR 45
Description: Safe-Green™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Safe-Green

G108-G 1.0 ml
EUR 45
Description: Safe-Green™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.