Mycoplasma Detection Kits Applied Biological Materials

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Description

Cryopreservation Medium by abm is a ready-to-use complete formulation suitable for the cryopreservation of various types of mammalian cells. With its unique formula this medium can be used to freeze cells cultured in classical media such as DMEM, DMEM/F12, RPMI 1640, and Ham’s F-12, as well as others.

This product is composed of 10% DMSO along with additional optimized components that will improve cell recovery after thawing. Every lot of Cryopreservation Medium by abm is tested for pH, sterility, and endotoxin.

Supplied by Applied Biological Materials (abm). Usually ships in 3 business days.

Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.

Mycoplasma Detection Kits – Conventional PCR

Mycoplasma contamination is a serious problem for laboratories due to its impact on cell properties and safety of biological products. Mycoplasma contaminations in cell cultures are unnoticeable (macroscopically as well as microscopically), making routine tests for reliable mycoplasma detection necessary. They represent a serious concern for academic and biopharmaceutical laboratories due to their effects on cell properties and on safety of biological products.

Mycoplasma (term often interchangeably used with Mollicutes, which include Mycoplasma, Acholeplasma, and Spiroplasma) are ubiquitous in the environment. In cell culture laboratories, 95 % of all mycoplasma infections in continuous cell lines are caused by as few as six contaminant species of bovine, swine, and human origin. These microbial agents easily spread from an infected cell culture batch to another by aerosol transmission or inadequate aseptic techniques.

Regular mycoplasma testing and control procedures help avoiding the costly consequences of a cell culture contamination and ensuring safe biopharmaceuticals and reliable scientific results. Consequently, most scientific publishers do not accept submission of cell-based studies without proof of the performed mycoplasma tests.

Compared to alternative methods, PCR-based mycoplasma detection kits are rapid, simple and do not require viable mycoplasma, special equipment, or extensive staff training.

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