ELISA assay for quantitative and qualitative analysis of exosomes
Methods.
Miller, I. V., & Grunewald, T. G. (2015). Tumor-derived exosomes: Tiny envelopes for big stories. Biology of the Cell, 107 (9), 287-305.
Hong, C. S., Muller, L., Boyiadzis, M., & Whiteside, T. L. (2014). Isolation and characterization of CD34 + blast-derived exosomes in acute myeloid leukemia. PLoS One, 9 (8), e103310.
Tetta, C., Ghigo, E., Silengo, L., Deregibus, M. C., & Camussi, G. (2013). Extracellular vesicles as an emerging mechanism of communication between cells. Endocrine, 44 (1), 11-19.
Vishnubhatla, I., Corteling, R., Stevanato, L., Hicks, C., & Sinden, J. (2014). Development of stem cell-derived exosomes as cell-free regenerative medicine. J. Circ. Biomark, 143, 2.
Logozzi, M., De Milito, A., Lugini, L., Borghi, M., Calabrò, L., Spada, M., … & Fais, S. (2009). High levels of exosomes expressing CD63 and caveolin-1 in the plasma of patients with melanoma. (4), e5219.
Exo-TEST: ready to use ELISA quantification kits
ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions.
ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is then performed using appropriate detection antibodies against exosome-associated antigens, which can be either generic or cell / tissue specific exosomes. Lyophilized exosome standards characterized by protein content and particle number (NTA) allow the quantification of an unknown sample by a standard calibration curve.
Applications: Capture and quantification of exosomes from human biofluids and culture media. Exosome complex profiling. Preclinical research on non-invasive biomarkers for the detection and monitoring of a number of pathological conditions (inflammation, cancer, neurodegeneration, etc.).
Advantages: Ready to use, no initial purification of exosomes is required. User-friendly and suitable for the analysis of multiple markers. Available in TEST format (limited to 3 ELISA strips, 24 wells).
Reference:
Musante, L., Tataruch-Weinert, D., Kerjaschki, D., Henry, M., Meleady, P., & Holthofer, H. (2017). Residual extracellular urinary vesicles in ultracentrifugation supernatants after enrichment by dialysis by hydrostatic filtration. Journal of Extracellular Vesicles, 6 (1), 1267896.
Foster, B. P., Balassa, T., Benen, T. D., Dominovic, M., Elmadjian, G. K., Florova, V., … & Kyvelidou, C. (2016). Extracellular vesicles in the blood, milk and body fluids of the female and male urogenital tracts and with special regard to reproduction. Critical Reviews in Clinical Laboratory Sciences, (just accepted), 1-47.
Tas, F., Karabulut, S., Tilgen Yasasever, C., & Duranyildiz, D. (2015). Clinical significance of serum caveolin -1 levels in patients with melanoma. International Journal of Dermatology.
Zarovni, N., Corrado, A., Guazzi, P., Zocco, D., Lari, E., Radano, G., … & Chiesi, A. (2015). Integrated isolation and quantitative analysis of exosome-transmitted proteins and nucleic acids using immunocapsura approaches.
 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
|||
| CAS410A-KIT | SBI | 1 kit (10 rxn) | EUR 798 |
 Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit |
|||
| CAS420A-KIT | SBI | 1 kit (10 rxn) | EUR 828 |
 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV100PA-KIT | SBI | 1 Kit | EUR 1358.4 |
 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV105PA-KIT | SBI | 1 Kit | EUR 1358.4 |
 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV120PA-KIT | SBI | 1 Kit | EUR 1358.4 |
 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV125PA-KIT | SBI | 1 Kit | EUR 1358.4 |
 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
| CAS700A-KIT | SBI | 10 rxn | EUR 1358.4 |
 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
| CAS720A-KIT | SBI | 10 rxn | EUR 1358.4 |
 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
| CAS740A-KIT | SBI | 10 rxn | EUR 1358.4 |
) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
|||
| CAS510A-KIT | SBI | 1 Kit | EUR 966 |
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV200PA-KIT | SBI | 1 Kit | EUR 1358.4 |
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV205PA-KIT | SBI | 1 Kit | EUR 1358.4 |
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV220PA-KIT | SBI | 1 Kit | EUR 1358.4 |
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV225PA-KIT | SBI | 1 Kit | EUR 1358.4 |
 Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|||
| CAS750A-KIT | SBI | 10 rxn | EUR 1358.4 |
 Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|||
| CAS770A-KIT | SBI | 10 rxn | EUR 1358.4 |
 Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|||
| CAS790A-KIT | SBI | 10 rxn | EUR 1358.4 |
 and Fast ligase (2.5ul)]) Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
|||
| CAS9LIG-KIT | SBI | 1 Kit | EUR 183.6 |
 6 each 30mL) Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
|||
| SPXMT-KIT | Scientific Laboratory Supplies | 30ML | EUR 237.12 |
) PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|||
| PIN320A-KIT | SBI | 1 Kit | EUR 5929.2 |
 6 each 30mL) Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
|||
| SPXHM-KIT | Scientific Laboratory Supplies | 30ML | EUR 245.1 |
) PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|||
| PIN340iPS-KIT | SBI | 1 Kit | EUR 5929.2 |
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
| GE620A-KIT | SBI | 1 kit | EUR 2558.4 |
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
| GE622A-KIT | SBI | 1 kit | EUR 2558.4 |
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
| GE624A-KIT | SBI | 1 kit | EUR 2558.4 |
 Proteasome Assay Kit |
|||
| 55R-1341 | Fitzgerald | 100 assays | EUR 784.8 |
|
Description: Assay Kit for detection of Proteasome in the research laboratory |
|||
 Calpain Assay Kit |
|||
| 55R-1342 | Fitzgerald | 100 assays | EUR 934.8 |
|
Description: Assay Kit for detection of Calpain in the research laboratory |
|||
 Glutathione Assay Kit |
|||
| 55R-1343 | Fitzgerald | 100 assays | EUR 784.8 |
|
Description: Assay Kit for detection of Glutathione in the research laboratory |
|||
Glutathione Assay Kit |
|||
| 55R-1354 | Fitzgerald | 100 assays | EUR 1074 |
|
Description: Assay Kit for detection of Glutathione activity in the research laboratory |
|||
 HDAC Assay Kit |
|||
| 55R-1371 | Fitzgerald | 100 assays | EUR 807.6 |
|
Description: Assay Kit for detection of HDAC in the research laboratory |
|||
HDAC Assay Kit |
|||
| 55R-1372 | Fitzgerald | 100 assays | EUR 807.6 |
|
Description: Assay Kit for detection of HDAC in the research laboratory |
|||
 HAT Assay Kit |
|||
| 55R-1373 | Fitzgerald | 100 assays | EUR 888 |
|
Description: Assay Kit for detection of HAT in the research laboratory |
|||
 SOD Assay Kit |
|||
| 55R-1374 | Fitzgerald | 100 assays | EUR 722.4 |
|
Description: Assay Kit for detection of SOD in the research laboratory |
|||
 HDAC3 Assay Kit |
|||
| 55R-1377 | Fitzgerald | 100 assays | EUR 831.6 |
|
Description: Assay Kit for detection of HDAC3 in the research laboratory |
|||
 HDAC8 Assay Kit |
|||
| 55R-1379 | Fitzgerald | 100 assays | EUR 826.8 |
|
Description: Assay Kit for detection of HDAC8 in the research laboratory |
|||
 ATP Assay Kit |
|||
| 55R-1380 | Fitzgerald | 100 assays | EUR 970.8 |
|
Description: Assay Kit for detection of ATP in the research laboratory |
|||
 ADP Assay Kit |
|||
| 55R-1381 | Fitzgerald | 100 assays | EUR 970.8 |
|
Description: Assay Kit for detection of ADP in the research laboratory |
|||