ELISA assay for quantitative and qualitative analysis of exosomes
Methods.
Miller, I. V., & Grunewald, T. G. (2015). Tumor-derived exosomes: Tiny envelopes for big stories. Biology of the Cell, 107 (9), 287-305.
Hong, C. S., Muller, L., Boyiadzis, M., & Whiteside, T. L. (2014). Isolation and characterization of CD34 + blast-derived exosomes in acute myeloid leukemia. PLoS One, 9 (8), e103310.
Tetta, C., Ghigo, E., Silengo, L., Deregibus, M. C., & Camussi, G. (2013). Extracellular vesicles as an emerging mechanism of communication between cells. Endocrine, 44 (1), 11-19.
Vishnubhatla, I., Corteling, R., Stevanato, L., Hicks, C., & Sinden, J. (2014). Development of stem cell-derived exosomes as cell-free regenerative medicine. J. Circ. Biomark, 143, 2.
Logozzi, M., De Milito, A., Lugini, L., Borghi, M., Calabrò, L., Spada, M., … & Fais, S. (2009). High levels of exosomes expressing CD63 and caveolin-1 in the plasma of patients with melanoma. (4), e5219.
Exo-TEST: ready to use ELISA quantification kits
ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions.
ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is then performed using appropriate detection antibodies against exosome-associated antigens, which can be either generic or cell / tissue specific exosomes. Lyophilized exosome standards characterized by protein content and particle number (NTA) allow the quantification of an unknown sample by a standard calibration curve.
Applications: Capture and quantification of exosomes from human biofluids and culture media. Exosome complex profiling. Preclinical research on non-invasive biomarkers for the detection and monitoring of a number of pathological conditions (inflammation, cancer, neurodegeneration, etc.).
Advantages: Ready to use, no initial purification of exosomes is required. User-friendly and suitable for the analysis of multiple markers. Available in TEST format (limited to 3 ELISA strips, 24 wells).
Reference:
Musante, L., Tataruch-Weinert, D., Kerjaschki, D., Henry, M., Meleady, P., & Holthofer, H. (2017). Residual extracellular urinary vesicles in ultracentrifugation supernatants after enrichment by dialysis by hydrostatic filtration. Journal of Extracellular Vesicles, 6 (1), 1267896.
Foster, B. P., Balassa, T., Benen, T. D., Dominovic, M., Elmadjian, G. K., Florova, V., … & Kyvelidou, C. (2016). Extracellular vesicles in the blood, milk and body fluids of the female and male urogenital tracts and with special regard to reproduction. Critical Reviews in Clinical Laboratory Sciences, (just accepted), 1-47.
Tas, F., Karabulut, S., Tilgen Yasasever, C., & Duranyildiz, D. (2015). Clinical significance of serum caveolin -1 levels in patients with melanoma. International Journal of Dermatology.
Zarovni, N., Corrado, A., Guazzi, P., Zocco, D., Lari, E., Radano, G., … & Chiesi, A. (2015). Integrated isolation and quantitative analysis of exosome-transmitted proteins and nucleic acids using immunocapsura approaches.
 QuantiChrom™ Zinc Assay Kit | |||
| DIZN-250 | BioAssay Systems | 250 | EUR 489 |
Description: For quantitative determination of zinc ion Zn2+ and evaluation of drug effects on zinc metabolism. Key Features: Sensitive and accurate. Uses 50 µL samples. Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible. Low interference in biological samples. No pretreatments are needed. Method: OD425nm. Samples: Serum, plasma, urine, saliva, food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.78 µg/dL (0.12 µM). | |||
 QuantiChrom™ Boron Assay Kit | |||
| DBOR-100 | BioAssay Systems | 100 | EUR 339 |
Description: For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm. | |||
 QuantiChrom™ TBARS Assay Kit | |||
| DTBA-100 | BioAssay Systems | 100 | EUR 249 |
Description: For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation. Key Features: Sensitive and accurate. Linear detection range: colorimetric assay 1 - 30 µM, fluorometric assay 0.1 - 1.5 µM MDA. Method: OD535nm, or FL530/550nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 80 min. Kit size: 100 tests. Detection limit: 0.1 µM. | |||
 QuantiChrom™ Biotin Assay Kit | |||
| DBIO-100 | BioAssay Systems | 100 | EUR 229 |
Description: For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM. | |||
 QuantiChrom™ Copper Assay Kit | |||
| DICU-250 | BioAssay Systems | 250 | EUR 479 |
Description: For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism. Key Features: Sensitive and accurate. Linear detection range 7 µg/dL (1.0 µM) to 300 µg/dL (47 µM) copper in 96-well plate assay. Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Method: OD359nm. Samples: Biological, environment, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 7 µg/dL (1.0 µM). | |||
 QuantiChrom™ Indole Assay Kit | |||
| DIND-100 | BioAssay Systems | 100 | EUR 199 |
Description: For quantitative determination of indole and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565nm. Samples: Bacterial growth media and other biological samples. Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 3 µM. | |||
 QuantiChrom™ Lipase Assay Kit | |||
| DLPS-100 | BioAssay Systems | 100 | EUR 469 |
Description: For quantitative determination of lipase enzyme activity. Key Features: Sensitive and accurate. Linear detection range 40 to 1600 U/L lipase activity in 96-well plate assay. Convenient and high throughput. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day. Method: OD412nm. Samples: Serum, plasma, saliva, urine etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 40 U/L. | |||
 QuantiChrom™ Urease Assay Kit | |||
| DURE-100 | BioAssay Systems | 100 | EUR 429 |
Description: For quantitative determination of urease activity and evaluation/screen for urease inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD670nm. Samples: Biological, environment etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.003 U/L. | |||
 QuantiFluo™ Ammonia Assay Kit | |||
| DNH3-200 | BioAssay Systems | 200 | EUR 189 |
Description: For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological (eg urine) and environ-mental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 200 tests. Detection limit: 12 µM. | |||
 QuantiFluo™ Protein Assay Kit | |||
| QFPR-200 | BioAssay Systems | 200 | EUR 159 |
Description: For quantitative determination of total protein. Key Features: Fast and sensitive. Assay is completed within a few minutes. Linear detection range of 0.05 - 200 µg/mL BSA. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological. Species: All. Procedure: Assay takes 10 min. Kit size: 200 tests. Detection limit: 50 ng/mL. | |||
 QuantiChrom™ Cobalt Assay Kit | |||
| DCBT-100 | BioAssay Systems | 100 | EUR 509 |
) QuantiChrom™ Urea Assay Kit (100T) | |||
| DIUR-100 | BioAssay Systems | 100 | EUR 199 |
Description: For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM). | |||
 QuantiChrom α-Amylase Assay Kit | |||
| DAMY-100 | BioAssay Systems | 100 | EUR 550.8 |
Description: Quantitative determination of α-amylase activity by colorimetric (595nm) method. Procedure: 10 min. Kit size: 100 tests. Detection limit: 10 U/L. Shelf life: 6 months. Shipping: ambient temp; storage: 4°C. | |||
 QuantiChrom™ Calcium Assay Kit | |||
| DICA-500 | BioAssay Systems | 500 | EUR 389 |
Description: For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca2+ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM). | |||
 QuantiChrom™ Indican Assay Kit | |||
| DIDC-01K | BioAssay Systems | 1000 | EUR 419 |
Description: For quantitative determination of indican (indoxyl sulfate) in urine samples. Key Features: Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 µmoles/L). Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Urine. Species: All. Procedure: Assay takes 10 min. Kit size: 1000 tests. Detection limit: 0.2 mg/dL. | |||
QuantiChrom™ Indican Assay Kit | |||
| DIDC-100 | BioAssay Systems | 100 | EUR 309 |
Description: For quantitative determination of indican (indoxyl sulfate) in urine samples. Key Features: Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 µmoles/L). Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Urine. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.2 mg/dL. | |||
 QuantiChrom™ Ethanol Assay Kit | |||
| DIET-500 | BioAssay Systems | 500 | EUR 489 |
Description: For quantitative determination of ethanol and alcohol metabolism. Key Features: Sensitive and accurate. Detection range 0.04 - 4% alcohol in 96-well plate assay. Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD580nm (Chemical). Samples: Alcoholic beverages etc. Species: All. Procedure: Assay takes 10 min. Kit size: 500 tests. Detection limit: 0.04%. | |||
 QuantiChrom™ Glucose Assay Kit | |||
| DIGL-100 | BioAssay Systems | 100 | EUR 319 |
Description: For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum and plasma samples. Method: OD630nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.7 mg/dL (39 µM). | |||
 QuantiChrom™ Phytase Assay Kit | |||
| DPHT-100 | BioAssay Systems | 100 | EUR 399 |
Description: A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples. Key Features: Simple and convenient: No complex detection reagents to mix. High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay. Fast and high-throughput: Homogeneous "mix-and-measure" assay allows for quantification of phytase activity within 60 minutes. Method: OD620nm. Samples: Enzyme extracts, agriculture and biological samples. Species: All (e.g. Wheat, Barley). Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.01 U/L. | |||
 QuantiChrom™ Sulfate Assay Kit | |||
| DSFT-200 | BioAssay Systems | 200 | EUR 379 |
Description: For quantitative determination of sulfate ion and evaluation of drug effects on sulfate metabolism. Key Features: Sensitive and accurate. Detection range 0.02 mM (0.19 mg/dL) to 2 mM (19.2 mg/dL) sulfate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Method: OD600nm. Samples: Serum, urine, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 200 tests. Detection limit: 20 µM. | |||
 QuantiChrom™ Protein Assay Kit | |||
| QCPR-500 | BioAssay Systems | 500 | EUR 249 |
Description: For quantitative determination of total protein. Key Features: Sensitive and accurate. Use 10 mL samples. Detection range 0.06 - 1.0 mg /mL protein in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (<0.1%) and SDS (< 0.1% SDS) do not interfere in the assay. Versatility: assays can be executed in 96-well plate or cuvet. Method: OD595nm (Bradford). Samples: Biological, food, agriculture etc. Species: All. Procedure: Assay takes 2 min. Kit size: 500 tests. Detection limit: 60 µg/mL. | |||
 Peroxide assay, colorimetric micro assay kit, 96 test, Quantitative | |||
| 1350-POX-1 | Alpha Diagnostics | 1 kit | EUR 489.6 |
 QuantiChrom™ Carbonyl Assay Kit | |||
| DCAR-100 | BioAssay Systems | 100 | EUR 349 |
Description: For quantitative determination of carbonyl groups or protein carbonyls and evaluation of drug modulators. Key Features: Fast and sensitive. Use of 100 µL sample. Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD375nm. Samples: Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 12 µM. | |||
 QuantiChrom™ Chromium Assay Kit | |||
| DCRM-250 | BioAssay Systems | 250 | EUR 459 |
Description: For quantitative determination of chromium Cr(VI). Key Features: Sensitive and accurate. Linear detection range of 20 - 2000 µg/L Chromium. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Biological (serum, plasma etc), environmental (water, soil etc), food and beverage samples. Species: All. Procedure: Assay takes 20 min. Kit size: 250 tests. Detection limit: 20 µg/L (0.38 µM, 0.02 ppm). | |||
 QuantiChrom™ Chloride Assay Kit | |||
| DICL-250 | BioAssay Systems | 250 | EUR 329 |
Description: For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl- in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD610nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.7 mg/dL (0.2 mM). | |||
 QuantiChrom™ Peroxide Assay Kit | |||
| DIOX-250 | BioAssay Systems | 250 | EUR 349 |
Description: For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism. Key Features: Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H2O2 in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD585nm. Samples: Serum, citrate-plasma, urine, cell lysate, culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 µM. | |||
 QuantiFluo™ Urokinase Assay Kit | |||
| DUKN-100 | BioAssay Systems | 100 | EUR 389 |
Description: For quantitative determination of urokinase activity determination in biological samples. Key Features: Safe. Non-radioactive assay. Fast. Assay is completed within a 15 minute reaction time. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to assay thousands of samples per day. Method: FL380/450 nm; Samples: Biological Samples (e.g. Urine and serum). Species: All. Procedure: assay takes 15min. Size: 100 tests. Detection Limit: 0.04 U/L. | |||
 Peroxidase assay, colorimetric micro assay kit, 96 test, Quantitative | |||
| 1360-PAS-1 | Alpha Diagnostics | 1 kit | EUR 634.8 |
 QuantiChrom™ Detergent Assay Kit. | |||
| DDTR-100 | BioAssay Systems | 100 | EUR 429 |
Description: For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. | |||
 QuantiChrom™ Bilirubin Assay Kit | |||
| DIBR-180 | BioAssay Systems | 180 | EUR 359 |
Description: For quantitative determination of bilirubin and evaluation of drug effects on bilirubin metabolism. Key Features: Sensitive and accurate. Detection limit 0.16 mg/dL bilirubin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD530nm. Samples: Serum. Species: All. Procedure: Assay takes 10 min. Kit size: 180 tests. Detection limit: 0.16 mg/dL. | |||
 QuantiChrom™ Magnesium Assay Kit | |||
| DIMG-250 | BioAssay Systems | 250 | EUR 409 |
Description: For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM). | |||
 QuantiChrom™ Phosphate Assay Kit | |||
| DIPI-500 | BioAssay Systems | 500 | EUR 349 |
Description: For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism. Key Features: Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals. Method: OD620nm (malachite green). Samples: Serum, urine, saliva, sweat, tissue culture, food, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 µg/dL (0.3 µM). | |||
 QuantiChrom™ Pectinase Assay Kit | |||
| DPEC-100 | BioAssay Systems | 100 | EUR 299 |
Description: For quantitative determination of pectinase activity determination in biological samples. Key Features: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. Simple and convenient 40 minute "Add-Mix-Measure" type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating required. The kit does not use toxic materials. Refer to the previously established DNS method. Method: OD600nm. Samples: Enzyme extracts, agriculture and biological samples. Species: Plant, fungal tissues, juice, bacteria, etc. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 9.2 U/L. | |||
 QuantiChrom™ Peroxidase Assay Kit | |||
| D2PD-100 | BioAssay Systems | 100 | EUR 339 |
Description: For quantitative determination of peroxidase enzyme activity. Key Features: Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 0.8 U/L. | |||
 QuantiChrom™ Creatinine Assay Kit | |||
| DICT-500 | BioAssay Systems | 500 | EUR 399 |
Description: For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM). | |||
 QuantiChrom™ Hemoglobin Assay Kit | |||
| DIHB-250 | BioAssay Systems | 250 | EUR 369 |
Description: For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism. Key Features: Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.9 mg/dL. | |||
 QuantiChrom™ Salicylate Assay Kit | |||
| DSALY-100 | BioAssay Systems | 100 | EUR 439 |
Description: For quantitative determination of salicylate in biological samples and beauty products, mouthwash, etc. Key Features: Fast and sensitive. Linear detection range: 0.8 mM (10.9 mg/dL) to 20 mM (274.2 mg/dL) salicylate with 20 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD560nm. Samples: Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 Tests. | |||