Category: Bile Acid Assay
Assessment of the oxidative status and goblet cell response during eimeriosis and after treatment of mice with magnesium oxide nanoparticles
Magnesium nanoparticles have been the main focus of examine over the previous few years due to its performance within the physique. Evaluation of the affect of magnesium oxide nanoparticles (MgNPs) on eimeriosis has but to be performed. The purpose of this examine was to see how MgNPs affected the parasite Eimeria papillata contaminated jejunum. To induce eimeriosis,…Read More
RFP-based method for real-time tracking of invasive bacteria in a heterogeneous population of cells
Quantification of bacterial invasion into eukaryotic cells is a prerequisite to unfold the molecular mechanisms of this vector’s perform to acquire insights for enhancing its effectivity. Invasion is historically quantified by antibiotic safety assays that require dilution plating and counting of colony-forming models rescued from contaminated cells. Nonetheless, to distinguish between connected and internalized micro…Read More
A Gene Cluster That Encodes Histone Deacetylase Inhibitors Contributes to Bacterial Persistence and Antibiotic Tolerance in Burkholderia thailandensis.
Persister cells are genetically identical variants in a bacterial population that have phenotypically modified their physiology to survive environmental stress. In bacterial pathogens, persisters are able to survive antibiotic treatment and reinfect patients in a frustrating cycle of chronic infection. To better define core persistence mechanisms for therapeutics development, we performed transcriptomics analyses of Burkholderia thailandensis…Read More
Tin oxide nanoparticles trigger the formation of amyloid β oligomers/protofibrils and underlying neurotoxicity as a marker of Alzheimer’s diseases
Alzheimer’s disease (AD) is known as one of the most common forms of dementia, and oligomerization of amyloid β (Aβ42) peptides can result in the onset of AD. Tin oxide nanoparticles (SnO2 NPs) showed several applications in biomedical fields can trigger unwanted interaction with proteins and inducing protein aggregation. Herein, we synthesized SnO2 NPs via the hydrothermal…Read More
GFP-Margatoxin, a Genetically Encoded Fluorescent Ligand to Probe Affinity of Kv1.3 Channel Blockers
Peptide pore blockers and their fluorescent derivatives are useful molecular probes to study the structure and functions of the voltage-gated potassium Kv1.3 channel, which is considered as a pharmacological target in the treatment of autoimmune and neurological disorders. We present Kv1.3 fluorescent ligand, GFP-MgTx, constructed on the basis of green fluorescent protein (GFP) and margatoxin…Read More
The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues
Cancer cells accumulate epigenomic aberrations that contribute to cancer initiation and progression by altering both the genomic stability and the expression of genes. The awareness of such alterations could improve our understanding of cancer dynamics and the identification of new therapeutic strategies and biomarkers to refine tumor classification and treatment. Formalin fixation and paraffin embedding…Read More
Ultrasonographic observation in combination with progesterone monitoring for detection of ovulation in Labrador Retrievers
Although it is well known that the ovulation occurs during a period of time after LH surge in dogs, there are few reports of observing the entire process of development, ovulation, and luteinization of each follicle. This study aimed to detect the ovulation kinetics by ultrasonography in combination with progesterone monitoring, and therefore identify the…Read More
Validation of the Applied Biosystems 7500 Fast Instrument for the Detection of Salmonellae with SureTect Salmonella Species PCR Kit.
The Thermo Scientific SureTect Salmonella species real-time PCR assay is a rapid alternative method designed for the detection of salmonellae in a wide range of foods, animal feeds, and production-environment samples. The assay has previously been validated according to the AOAC Research Institute Performance Tested Methods(SM) program using Thermo Scientific PikoReal™ PCR cycler and Thermo…Read More
Utilization of a direct sandwich ELISA kit for rapid diagnosis of adenovirus hemorrhagic cystitis in a patient with adult T-cell leukemia
We report on a case of adenovirus hemorrhagi cystitis that developed in a 49-year-old woman during intensive chemotherapy for adult T-cell leukemia. Although rapid etiologic diagnosis is essential for the effective management of viral hemorrhagic cystitis, the isolation of adenovirus from urine is often too time-cousuming. We detected the adenovirus antigen in the patient’s urine…Read More
Crystal structure and energetic features of the cocrystal of carbamazepine with 3,5-dinitrobenzoic acid.
The synthesis and detailed description of the crystal structure and energetic features of the 1:1 cocrystal of carbamazepine (5H-dibenzo[b,f]azepine-5-carboxamide, CBZ) with 3,5-dinitrobenzoic acid (35DNBA), i.e. C15H12N2O·C7H4N2O6, are reported. The CBZ and 35DNBA molecules are packed in alternately arranged layers. Two characteristic R22(8) and R22(16) hydrogen-bond ring motifs have been found. The supramolecular architecture, besides the…Read More