Development of a qPCR-based method for counting overwintering spruce budworm (Choristoneura fumiferana) larvae collected during fall surveys and for assessing their natural enemy load: a proof-of-concept study
The Impression of Sample Website, Illness Size, and the Presence of Pneumonia on the Detection of SARS-CoV-2 by Precise-time Reverse Transcription PCR
Background: The effectivity of real-time reverse transcription polymerase chain response (rRT-PCR) for SARS-CoV-2 varies with sampling website online(s), illness stage, and an an infection website online.
Methods: Unilateral nasopharyngeal, nasal midturbinate, throat swabs, and saliva have been concurrently sampled for SARS-CoV-2 rRT-PCR from suspected or confirmed circumstances of COVID-19. True positives have been outlined as victims with a minimal of 1 SARS-CoV-2 detected by rRT-PCR from any website online on the evaluation day or at any time stage thereafter, until discharge. Diagnostic effectivity was assessed and extrapolated for website online combos.
Outcomes: We evaluated 105 victims; 73 had vigorous SARS-CoV-2 an an infection. Complete, nasopharyngeal specimens had the perfect scientific sensitivity at 85%, adopted by throat, 80%, midturbinate, 62%, and saliva, 38%-52%. Medical sensitivity for nasopharyngeal, throat, midturbinate, and saliva was 95%, 88%, 72%, and 44%-56%, respectively, if taken ≤7 days from onset of illness, and 70%, 67%, 47%, 28%-44% if >7 days of illness.
Evaluating victims with greater respiratory tract an an infection (URTI) vs pneumonia, scientific sensitivity for nasopharyngeal, throat, midturbinate, and saliva was 92% vs 70%, 88% vs 61%, 70% vs 44%, 43%-54% vs 26%-45%, respectively. A mixture of nasopharyngeal plus throat or midturbinate plus throat specimen afforded complete scientific sensitivities of 89%-92%; this rose to 96% for people with URTI and 98% for people ≤7 days from illness onset.
Conclusions: Nasopharyngeal specimens, adopted by throat specimens, provide the perfect scientific sensitivity for COVID-19 prognosis in early illness. Medical sensitivity improves and is comparable when each midturbinate or nasopharyngeal specimens are blended with throat specimens. Larger respiratory specimens perform poorly if taken after the first week of illness or if there could also be pneumonia.
Propidiummonoazide for viable Salmonella enterica detection by PCR and LAMP assays in comparison with RNA-based RT-PCR, RT-LAMP, and culture-based assays
Speedy and delicate detection of keep/infectious foodborne pathogens is urgently needed with a function to forestall outbreaks and meals remembers. This analysis aimed to (1) think about the incorporation of propidiummonoazide (PMA) into PCR or LAMP assays to selectively detect viable Salmonella Enteritidis following sublethal heat or UV treatment,
and autoclave sterilization; and (2) consider the detection of PMA-PCR and PMA-LAMP to DNA-based PCR and LAMP (with out PMA), RNA-based RT-PCR and RT-LAMP, and culture-based methods. Nucleic acids (DNA or RNA) from 1-mL S. Enteritidis samples have been used for PCR, RT-PCR, LAMP, and RT-LAMP assays. Serially diluted samples have been plated on Xylose Lysine Tergitol-4 agar for cultural enumeration.
Comparable detection of in a single day cultured S. Enteritidis was obtained by PMA-PCR, PCR, and RT-PCR, though 1 to 2 log a lot much less delicate than cultural assays. PMA-LAMP and RT-LAMP confirmed associated detection of in a single day cultures, being 1 to 2 log a lot much less delicate than the LAMP assay, and ∼4 log decrease than culture-based detection. Autoclaved S. Enteritidis did not test optimistic by RNA-based methods or PMA-PCR, nonetheless PMA-LAMP confirmed detection of 1 log CFU/mL.PMA-PCR and RT-PCR confirmed comparable detection of sublethal heat-treated cells to cultural assays, whereas PMA-LAMP confirmed 1 to 2 log a lot much less detection.
Our outcomes suggest that PMA-PCR and PMA-LAMP assays normally will not be applicable for selective viable cell detection after UV treatment. Whereas PMA-LAMP assay needs optimization, PMA-PCR displays promise for keep/viable S. Enteritidis detection. PMA-PCR displays potential for routine testing in the meals commerce with outcomes inside 1-day, albeit counting on the inactivation approach employed.
Enchancment and Evaluation of an iiPCR Assay for Salmonella and Shigella Detection on a Space-Deployable PCR System
Background: Salmonella and Shigella are typically associated to fecal-oral transmission and set off large-scale outbreaks in centralized catering objects and, subsequently, should be steadily and strictly monitored, notably amongst meals handlers. Nonetheless, no specific and delicate on-site detection approach is on the market until now.
Methods: On this analysis, an insulated isothermal PCR assay for the detection of Salmonella and Shigella on a field-deployable PCR system was developed. Specificity, sensitivity, reproducibility, and scientific accuracy of the assay have been characterised and evaluated.
Outcomes: The insulated isothermal PCR assay could be completed inside 58 minutes with minimal pretreatment needed. The assay was specific and with good reproducibility. The limit of detection was 103 CFU/mL and 101 CFU/mL for Salmonella and Shigella,
respectively, which was just like multiplex real-time PCR. Mock on-site scientific evaluation outcomes confirmed that the analytical sensitivity and specificity of the insulated isothermal PCR assay have been 100% and 96.6%, whereas the optimistic predictive price and unfavourable predictive price have been 94.1% and 100%, respectively.
Conclusion: Based on our outcomes, we think about that the assay developed herein may serve in its place approach for preliminary screening and provide a helpful platform for the on-site detection of Salmonella and Shigella, notably in resource-limited and rising nations.
 Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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| DD2R | Detroit R&D | 1 Kit | EUR 516 |
 Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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| DD2H | Detroit R&D | 1 Kit | EUR 516 |
 Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit |
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| DD2M | Detroit R&D | 1 Kit | EUR 516 |
 qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-360L | MiTeGen | 10 x 1,25 ml | EUR 818 |
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Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-360S | MiTeGen | 2 x 1,25 ml | EUR 205 |
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Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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 qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-363L | MiTeGen | 10 x 1,25 ml | EUR 982 |
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Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-363S | MiTeGen | 2 x 1,25 ml | EUR 246 |
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Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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 qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-362L | MiTeGen | 10 x 1,25 ml | EUR 818 |
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Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-362S | MiTeGen | 2 x 1,25 ml | EUR 205 |
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Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
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 qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-361L | MiTeGen | 10 x 1,25 ml | EUR 818 |
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Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-361S | MiTeGen | 2 x 1,25 ml | EUR 205 |
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Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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 qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-364L | MiTeGen | 10 x 1,25 ml | EUR 982 |
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Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-364S | MiTeGen | 2 x 1,25 ml | EUR 246 |
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Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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 qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-365L | MiTeGen | 10 x 1,25 ml | EUR 982 |
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Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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| M-PCR-365S | MiTeGen | 2 x 1,25 ml | EUR 246 |
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Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
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 Tiamulin ELISA Kit, Quantitative |
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| DEIA036 | Creative Diagnostics | 96T | EUR 1414.8 |
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Description: This kit is intended for quantitative analysis of tiamulin residue in animal tissue (pork and chicken, etc). |
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 Human RBP4 Quantitative ELISA Kit |
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| LF-EK70030 | Abfrontier | 1×96T | EUR 722.4 |
 Rat C3 ELISA Kit, 96 tests, Quantitative |
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| 6230 | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Rat IgA ELISA kit 96 tests, Quantitative |
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| 6410-10 | Alpha Diagnostics | 1 kit | EUR 607.2 |
 Rat IgG ELISA Kit, 96 tests, Quantitative |
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| 6420 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Rat IgE ELISA Kit, 96 tests, Quantitative |
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| 6470 | Alpha Diagnostics | 1 kit | EUR 927.6 |
 Rat IgM ELISA Kit, 96 tests, Quantitative |
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| 6480 | Alpha Diagnostics | 1 Kit | EUR 854.4 |
 Cat IgG ELISA Kit, 96 tests, Quantitative |
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| 300-100-CGG | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Cat IgM ELISA kit 96 tests, Quantitative |
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| 300-170-CGM | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Cat IgA ELISA Kit, 96 tests, Quantitative |
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| 300-180-CGA | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Cat IgE ELISA Kit, 96 tests, Quantitative |
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| 300-190-CGE | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Pig IgM ELISA Kit, 96 tests, Quantitative |
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| 9080 | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Dog IgG ELISA Kit, 96 tests, Quantitative |
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| 400-100-DGG | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 Dog IgM ELISA Kit, 96 tests, Quantitative |
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| 400-170-DGM | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 Dog IgA ELISA Kit, 96 tests, Quantitative |
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| 400-180-DGA | Alpha Diagnostics | 1 Kit | EUR 854.4 |
 Dog IgE ELISA Kit, 96 tests, Quantitative |
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| 400-190-DGE | Alpha Diagnostics | 1 Kit | EUR 854.4 |
 Lactate ELISA Kit, 96 tests, Quantitative |
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| 10795 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
 Rat IgG1 ELISA Kit, 96 tests, Quantitative |
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| 6430 | Alpha Diagnostics | 1 Kit | EUR 854.4 |
 Rat VEGF ELISA Kit, 96 tests, quantitative |
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| 200-840-VER | Alpha Diagnostics | 1 kit | EUR 927.6 |
 Goat IgG ELISA Kit, 96 tests, Quantitative |
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| 7520 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Goat IgM ELISA Kit, 96 tests, Quantitative |
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| 7530 | Alpha Diagnostics | 1 kit | EUR 927.6 |
 Goat IgA ELISA Kit, 96 tests, Quantitative |
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| 7540 | Alpha Diagnostics | 1 kit | EUR 1000.8 |
 Goat IgE ELISA Kit, 96 tests, Quantitative |
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| 7550 | Alpha Diagnostics | 1 kit | EUR 1000.8 |
 Oxytocin ELISA Kit, 96 tests, Quantitative |
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| 10790 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
 Mouse IgA ELISA Kit, 96 tests, Quantitative |
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| 6310 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Mouse IgG ELISA Kit, 96 tests, Quantitative |
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| 6320 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Mouse IgE ELISA Kit, 96 tests, Quantitative |
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| 6370 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 Mouse IgM ELISA Kit, 96 tests, Quantitative |
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| 6380 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 Rat IgG2a ELISA Kit, 96 tests, Quantitative |
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| 6440 | Alpha Diagnostics | 1 Kit | EUR 578.4 |
 Rat IgG2b ELISA Kit, 96 tests, Quantitative |
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| 6450 | Alpha Diagnostics | 1 Kit | EUR 634.8 |
 Human IgM ELISA Kit, 96 tests, Quantitative |
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| 1760 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 Human IgA ELISA Kit, 96 tests, Quantitative |
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| 1780 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Human IgG ELISA Kit, 96 tests, Quantitative |
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| 1750 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 Sheep IgG ELISA Kit, 96 tests, Quantitative |
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| 7620 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 Sheep IgM ELISA Kit, 96 tests, Quantitative |
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| 7630 | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Sheep IgA ELISA Kit, 96 tests, Quantitative |
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| 7640 | Alpha Diagnostics | 1 kit | EUR 1000.8 |
 Sheep IgE ELISA Kit, 96 tests, Quantitative |
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| 7650 | Alpha Diagnostics | 1 kit | EUR 1000.8 |
 Horse IgA ELISA Kit, 96 tests, Quantitative |
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| 7720 | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Horse IgG ELISA Kit, 96 tests, Quantitative |
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| 7730 | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Horse IgM ELISA Kit, 96 tests, Quantitative |
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| 7740 | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Horse IgE ELISA Kit, 96 tests, Quantitative |
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| 7750 | Alpha Diagnostics | 1 kit | EUR 927.6 |
 Camel IgG ELISA Kit, 96 tests, Quantitative |
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| 7820 | Alpha Diagnostics | 1 kit | EUR 854.4 |
 G. Pig IgA ELISA Kit, 96 tests, Quantitative |
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| 7410 | Alpha Diagnostics | 1 kit | EUR 854.4 |
 G. Pig IgG ELISA Kit, 96 tests, Quantitative |
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| 7420 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 G. Pig IgM ELISA Kit, 96 tests, Quantitative |
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| 7430 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Mouse IgG1 ELISA Kit, 96 tests, Quantitative |
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| 6330 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Mouse IgG3 ELISA Kit, 96 tests, Quantitative |
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| 6360 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Rabbit IgA ELISA Kit, 96 tests, Quantitative |
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| 6510 | Alpha Diagnostics | 1 kit | EUR 927.6 |
 Rabbit IgM ELISA Kit, 96 tests, Quantitative |
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| 6580 | Alpha Diagnostics | 1 kit | EUR 708 |
 Monkey IgA ELISA Kit, 96 tests, Quantitative |
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| 7010 | Alpha Diagnostics | 1 Kit | EUR 854.4 |
 Vitamin A ELISA Kit, 96 tests, Quantitative |
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| 10760 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
 Vitamin B1 ELISA Kit, 96 tests, Quantitative |
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| 10765 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
 Vitamin C ELISA Kit, 96 tests, Quantitative |
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| 10770 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
 Vitamin E ELISA Kit, 96 tests, Quantitative |
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| 10775 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
 Bovine IgG ELISA Kit, 96 tests, Quantitative |
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| 8010 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 Turkey IgG ELISA Kit, 96 tests, Quantitative |
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| 8020 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Bovine IgA ELISA Kit, 96 tests, Quantitative |
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| 8075 | Alpha Diagnostics | 1 Kit | EUR 854.4 |
 Bovine IgM ELISA Kit, 96 tests, Quantitative |
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| 8080 | Alpha Diagnostics | 1 Kit | EUR 854.4 |
 Horse Fab2 ELISA Kit, 96 tests, Quantitative |
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| 7710-Fab | Alpha Diagnostics | 1 kit | EUR 854.4 |
 Mouse VEGF ELISA Kit, 96 tests, Quantitative |
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| 100-230-VEM | Alpha Diagnostics | 1 Kit | EUR 854.4 |
 Human CCL2 ELISA Kit, 96 tests, Quantitative |
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| HCC-0L2-105 | Alpha Diagnostics | 1 kit | EUR 578.4 |
 Human CCL3 ELISA Kit, 96 tests, Quantitative |
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| HCC-0L3-110 | Alpha Diagnostics | 1 kit | EUR 578.4 |
 Human CCL4 ELISA Kit, 96 tests, Quantitative |
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| HCC-0L4-115 | Alpha Diagnostics | 1 kit | EUR 578.4 |
 Human CD93 ELISA Kit, 96 tests, Quantitative |
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| HCD-093-120 | Alpha Diagnostics | 1 kit | EUR 578.4 |
 Mouse CCL2 ELISA Kit, 96 tests, Quantitative |
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| MCC-0L2-100 | Alpha Diagnostics | 1 kit | EUR 578.4 |
 Mouse CCL3 ELISA Kit, 96 tests, Quantitative |
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| MCC-0L3-100 | Alpha Diagnostics | 1 kit | EUR 607.2 |
 Protein-A ELISA Kit, 480 tests, Quantitative |
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| 800-110-PRA-5 | Alpha Diagnostics | 1 pk | EUR 3714 |
 Rat IL-13 ELISA Kit, 96 tests, Quantitative |
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| RIL-013-100 | Alpha Diagnostics | 1 kit | EUR 578.4 |
 Vasopressin ELISA Kit, 96 tests, Quantitative |
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| 10800 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
 Mouse IgG2a ELISA Kit, 96 tests, Quantitative |
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| 6340 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Mouse IgG2b ELISA Kit, 96 tests, Quantitative |
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| 6350 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Rat Albumin ELISA Kit, 96 tests, Quantitative |
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| 6400 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Chicken IgM ELISA Kit, 96 tests, Quantitative |
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| 6040 | Alpha Diagnostics | 1 kit | EUR 781.2 |
