Ae1 Ae3 Antibody Antibodies Assay Kits Bafilomycin A1 Bile Acid Assay Biology Cells Canine Albumin cDNA Choline Acetyltransferase Antibody Clia Kits Culture Cells Deoxycholic Acid Sodium Salt Devices DNA DNA Templates DNA Testing Enzymes Equipments Exosomes F Actin Antibody Fto Antibody Gels Glycodeoxycholic Acid Gsk3 Alpha Hama Antibodies Hdac Activity Assay Isotypes Laminin Alpha 5 Medium & Serums Mip 1B Mmp Activity Assay Mothers Against Decapentaplegic Mouse Albumin Elisa Panel Particles PCR Pcr Kits Pepstatin A Peptides Phospho 4Ebp1 Primary Antibodies Reagents Recombinant Proteins Ria Kits Secreted Alkaline Phosphatase Soft Agar Tgf Alpha Antibody Tgf Alpha Elisa Trypsin Activity Assay Tubastatin A Uric Acid Assay Valproic Acid Sodium Salt Vi Alpha
A New Specific and Sensitive RT-qPCR Method Based on Splinted 5′ Ligation for the Quantitative Detection of RNA Species Shorter than microRNAs
Not too long ago, we found a brand new household of unusually brief RNAs mapping to five.8S ribosomal RNA (rRNA) and which we named dodecaRNAs (doRNAs), based on the variety of core nucleotides (12 nt) their members include. To verify these small RNA-sequencing (RNA-Seq) knowledge, validate the existence of the 2 overly ample doRNAs-the minimal core 12-nt doRNA sequence and its + 1-nt variant bearing a 5′ Cytosine, C-doRNA-and streamline their evaluation, we developed a brand new particular and delicate splinted 5′ ligation reverse transcription (RT)-quantitative polymerase chain response (qPCR) technique. This technique relies on a splint-assisted ligation of an adapter to the 5′ finish of doRNAs, adopted by RT-qPCR amplification and quantitation. Our optimized protocol, which can discriminate between doRNA, C-doRNA, mutated and precursor sequences, can precisely detect as little as 240 copies and is quantitatively linear over a variety of seven logs. This technique offers a novel instrument to increase and facilitate research exploring the molecular and mobile biology of RNA species shorter than microRNAs.
Victims with Preliminary Damaging RT-PCR and Typical Imaging of COVID-19: Medical Implications
The sensitivity of reverse transcriptase polymerase chain response (RT-PCR) has been questioned ensuing from unfavourable ends in some victims who’ve been strongly suspected of getting coronavirus sickness 2019 (COVID-19). The aim of our analysis was to analysis the prognosis of contaminated victims with preliminary unfavourable RT-PCR inside the emergency division (ED) in the midst of the COVID-19 outbreak. This analysis included two cohorts of grownup inpatients admitted into the ED. All victims who’ve been suspected to be contaminated with SARS-CoV-2 and who underwent a typical chest CT imaging have been included.
Thus, we studied two distinct cohorts: victims with optimistic RT-PCR (PCR+) and other people with unfavourable preliminary RT-PCR (PCR-). The data have been analyzed using Bayesian methods. We included 66 victims inside the PCR- group and 198 inside the PCR+ group. The baseline traits did not differ moreover on the subject of a proportion of lower energy respiratory sickness inside the PCR- group. We well-known a a lot much less excessive scientific presentation inside the PCR- group (lower respiratory price, lower oxygen need and mechanical air move requirement). Hospital mortality (9.1% vs. 9.6%) did not differ between the two groups. No matter an initially a lot much less vital scientific presentation, the mortality of victims contaminated by SARS-CoV-2 with a unfavourable RT-PCR did not differ from these with optimistic RT-PCR.
Seven Years Leptospirosis Comply with-Up in a Important Care Unit of a French Metropolitan Hospital; Position of Actual Time PCR for a Fast and Acute Analysis
(1) Background: Leptospirosis an an infection may end up in a lot of organ failure, requiring hospitalization in an intensive care unit for supportive care, along with initiation of an tailor-made antibiotic treatment. Attaining a quick prognosis is decisive inside the administration of these victims.
(2) Methods: We present proper right here a consider of leptospirosis circumstances acknowledged inside the intensive care unit of our hospital over seven years. Medical and natural info have been gathered, and we in distinction the variations on the subject of diagnostic method.
(3) Outcomes: Molecular biology method by Polymerase Chain Response (PCR) allowed quick and reliable prognosis when carried out inside the first days after the indicators began. Moreover, we acknowledged that sampling blood and urine for PCR was further atmosphere pleasant than performing PCR on only one type of natural sample.
(4) Conclusions: Our outcomes affirm the effectivity of PCR for the brief prognosis of leptospirosis and advocate that testing every blood and urine early inside the sickness could improve prognosis.
A Comparative Analysis of Some Procedures for Isolation of Fruit DNA of Sufficient Prime quality for PCR-Based totally Assays
- Meals fraud has been and nonetheless is a matter inside the meals commerce. It is detectable by a lot of approaches, harking back to extreme effectivity liquid chromatography (HPLC), chemometric assays, or DNA-based methods, each with its private drawbacks. This work addresses one primary drawback of DNA-based methods, significantly their sensitivity to inhibitors contained significantly matrices from which DNA is isolated.
- We examined 5 enterprise kits and one in-house method characterised by alternative routes of sample homogenization and DNA seize and purification.
- Using these methods, DNA was isolated from 10 utterly totally different fruit species usually utilized in plant-based foodstuffs. The usual of the DNA was evaluated by UV-VIS spectrophotometry. Two sorts of qPCR assays have been used for DNA top quality testing: (i) Method explicit for plant ITS2 space, (ii) methods explicit for explicit individual fruit species.
- Based totally totally on the outcomes of real-time PCR assays, we have now been able to find two column-based kits and one magnetic carrier-based tools, which continuously provided fruit DNA isolates of sufficient top quality for PCR-based assays useful for routine analysis and identification of explicit individual fruit species in meals merchandise.
One-Step Quantitative RT-PCR Assay with Armored RNA Controls for Detection of SARS-CoV-2
COVID-19 has develop to be pandemic since March, 11, 2020. Thus, progress and integration in clinics of fast and delicate diagnostic devices is essential. The aim of the analysis was a progress and evaluation of a one-step RT-qPCR assay (COVID-19 Amp) for SARS-CoV-2 detection with an armored optimistic administration and inside controls constructed from synthetic MS2-phage-based RNA particles.
The COVID-19 Amp assay prohibit of detection was 103 copies/ml, the analytical specificity was 100%. 109 natural samples have been examined using COVID-19 Amp and WHO-based assay. Discordance in 9 samples was observed (unfavourable by the WHO-based assay) and discordant samples have been retested as optimistic primarily based on the outcomes obtained from the Vector-PCRrv-2019-nCoV-RG assay.
The developed COVID-19 Amp assay has extreme sensitivity and specificity, comprises virus particles-based controls, provides the direct definition of SARS-CoV-2 RdRp gene partial sequence, and is acceptable for any hospital and laboratory equipped for RT-qPCR. This textual content is protected by copyright. All rights reserved.
 lyophilised real-time PCR Master Mix for dual labeled probes |
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| M-PCR-156L | MiTeGen | 960 reactions x 20 µl | EUR 900.68 |
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Description: lyophilised real-time PCR Master Mix for dual labeled probes |
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lyophilised real-time PCR Master Mix for dual labeled probes |
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| M-PCR-156S | MiTeGen | 192 reactions x 20 µl | EUR 225.3 |
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Description: lyophilised real-time PCR Master Mix for dual labeled probes |
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 Real Time RT-PCR Kit) Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
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| RR-0478-02 | Liferiver | 25 tests/kit | EUR 1189.2 |
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Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
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 0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS |
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| PCR-0108-LP-RT-C | CORNING | 125/pk | EUR 850.8 |
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Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
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 0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS |
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| PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 924 |
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Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
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 hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit |
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| 20-abx097601 | Abbexa |
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 dsGreen for Real-Time PCR, 100×, 1 mL |
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| 41010 | Lumiprobe | 1 mL | EUR 298.8 |
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| MOL2700 | Scientific Laboratory Supplies | EACH | EUR 30805.08 |
 lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173L | MiTeGen | 960 reactions x 20 µl | EUR 789.36 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173S | MiTeGen | 192 reactions x 20 µl | EUR 197.34 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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 hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097612 | Abbexa |
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 rno-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097920 | Abbexa |
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 dsGreen for Real-Time PCR, 100×, 2 mL |
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| 61010 | Lumiprobe | 2 mL | EUR 435.6 |
 dsGreen for Real-Time PCR, 100×, 10 mL |
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| 81010 | Lumiprobe | 10 mL | EUR 1042.8 |
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| 71010 | Lumiprobe | 5 mL | EUR 750 |
 rno-let-7a-1* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097919 | Abbexa |
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| 20-abx097924 | Abbexa |
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) PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA) |
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| 31036 | Biotium | 1kit | EUR 478.8 |
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Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) |
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| 31050 | Biotium | 1kit | EUR 513.6 |
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Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) |
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| 31051 | Biotium | 1kit | EUR 478.8 |
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Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip) |
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| 31053 | Biotium | 1kit | EUR 513.6 |
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Description: Minimum order quantity: 1 unit of 1kit |
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 hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097603 | Abbexa |
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 hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097604 | Abbexa |
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| 20-abx097607 | Abbexa |
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| 20-abx097608 | Abbexa |
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| 20-abx097611 | Abbexa |
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 hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097613 | Abbexa |
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| 20-abx097614 | Abbexa |
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 hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097615 | Abbexa |
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| 20-abx097616 | Abbexa |
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 hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097617 | Abbexa |
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 hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097618 | Abbexa |
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 hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097619 | Abbexa |
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 hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097620 | Abbexa |
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 hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097622 | Abbexa |
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 hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097625 | Abbexa |
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 hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097626 | Abbexa |
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 hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097627 | Abbexa |
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 hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097628 | Abbexa |
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 hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097629 | Abbexa |
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 hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097630 | Abbexa |
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 hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097631 | Abbexa |
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 hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097632 | Abbexa |
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 hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097633 | Abbexa |
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 hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097634 | Abbexa |
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 hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097636 | Abbexa |
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 hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097637 | Abbexa |
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 hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097638 | Abbexa |
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 hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097639 | Abbexa |
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 hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097640 | Abbexa |
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 hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097642 | Abbexa |
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 hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097643 | Abbexa |
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 hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097644 | Abbexa |
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 hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097645 | Abbexa |
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 hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097647 | Abbexa |
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 hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097648 | Abbexa |
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 hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097650 | Abbexa |
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 hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097651 | Abbexa |
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 hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097652 | Abbexa |
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 hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097653 | Abbexa |
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 hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097654 | Abbexa |
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 hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097657 | Abbexa |
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 hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097659 | Abbexa |
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 hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097661 | Abbexa |
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 hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097662 | Abbexa |
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 hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097663 | Abbexa |
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 hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097664 | Abbexa |
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 hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097665 | Abbexa |
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 hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097666 | Abbexa |
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 hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097667 | Abbexa |
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 hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097669 | Abbexa |
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 hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097671 | Abbexa |
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 hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097673 | Abbexa |
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 hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097675 | Abbexa |
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 hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097676 | Abbexa |
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 hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097677 | Abbexa |
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