Tag: particles of a liquid
Comparison between Whatman FTA Elute Cards and Conventional Swab for the Detection of Pathogenic Enteric Bacteria Using an RT-qPCR Assay
The emergence of outbreaks of foodborne sickness is carefully related to meals contamination attributable to varied enteric pathogens, resembling Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus. The management of enteric pathogens poses a problem attributable to the truth that these pathogens can persist for an extended time frame within the surroundings. The fast detection of pathogenic…
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DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencing
DNA aptamers generated by cell-SELEX towards bacterial cells have gained elevated curiosity as novel and cost-effective affinity reagents for cell labelling, imaging and biosensing. Right here we describe the choice and identification of DNA aptamers for bacterial cells utilizing a mixed method based mostly on cell-SELEX, state-of-the-art functions of quantitative real-time PCR (qPCR), next-generation sequencing…
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