RFP-based method for real-time tracking of invasive bacteria in a heterogeneous population of cells

Quantification of bacterial invasion into eukaryotic cells is a prerequisite to unfold the molecular mechanisms of this vector’s perform to acquire insights for enhancing its effectivity. Invasion is historically quantified by antibiotic safety assays that require dilution plating and counting of colony-forming models rescued from contaminated cells. Nonetheless, to distinguish between connected and internalized micro…

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Monitoring and Surveillance of Aerial Mycobiota of Rice Paddy through DNA Metabarcoding and qPCR

Monitoring and Surveillance of Aerial Mycobiota of Rice Paddy through DNA Metabarcoding and qPCR

The airborne mycobiota has been understudied compared with the mycobiota current in different agricultural environments. Conventional, culture-based strategies enable the research of a small fraction of the organisms current within the ambiance, thus lacking vital data. On this research, the aerial mycobiota in a rice paddy has been examined throughout the cropping season (from June…

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