computablegenomix

Auto-qPCR; a python-based web app for automated and reproducible analysis of qPCR data

Quantifying modifications in DNA and RNA ranges is crucial in quite a few molecular biology protocols. Quantitative actual time PCR (qPCR) strategies have developed to grow to be commonplace, nonetheless, information evaluation contains many time-consuming and cumbersome steps, which might result in errors and misinterpretation of knowledge. To handle these bottlenecks, now we have developed…

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computablegenomix

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR

Quite a few RT-qPCR assays for the detection of SARS-CoV-2 have been printed and are listed by the WHO as advisable assays. Moreover, quite a few business assays with undisclosed primer and probe sequences are available on the market. Because the SARS-CoV-2 pandemic progresses, the virus accrues mutations, which in some circumstances – as seen…

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