Identification and three-dimensional reconstruction of oxytocin receptor expressing astrocytes in the rat and mouse brain

Right here, we current a step-by-step protocol for three-dimensional reconstruction of astrocyte morphology, utilized to the central amygdala oxytocin receptor-expressing astrocytes. This contains RNAse-free perfusion, mixture of RNAscope and immunohistochemistry, and confocal imaging. This protocol supplies detailed details about tissue dealing with and a complete description of the RNAScope approach to label rat and mouse…

Read More

Normalization of SARS-CoV-2 viral load via RT-qPCR provides higher-resolution data for comparison across time and between patients

The 2020 pandemic has reworked the world and elicited hundreds of research to raised perceive the SARS-CoV-2 virus. Viral load has been a typical measure to observe remedy therapies and affiliate viral dynamics with affected person outcomes; nevertheless, strategies related to viral load have diverse throughout research. These variations have the potential to sacrifice the…

Read More