Tag: vi alpha tags
Author Correction: A simple and robust real-time qPCR method for the detection of PIK3CA mutations
Background: Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has precipitated a pandemic of pneumonia spreading all over the world, resulting in critical threats to public well being and attracting monumental consideration. There’s an pressing want for delicate diagnostic testing implementation to regulate and handle SARS-CoV-2 in public well being laboratories. The quantitative reverse transcription PCR (RT-qPCR) assay is…
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Ultra-sensitive AAV capsid detection by immunocapture-based qPCR following factor VIII gene transfer
Adeno-associated virus (AAV)-based gene remedy vectors are replication-incompetent and thus pose minimal threat for horizontal transmission or launch into the surroundings. In research with AAV5-FVIII-SQ (valoctocogene roxaparvovec), an investigational gene remedy for hemophilia A, residual vector DNA was detectable in blood, secreta, and excreta, nevertheless it remained unclear how lengthy structurally intact AAV5 vector capsids…
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