Identification and three-dimensional reconstruction of oxytocin receptor expressing astrocytes in the rat and mouse brain

Right here, we current a step-by-step protocol for three-dimensional reconstruction of astrocyte morphology, utilized to the central amygdala oxytocin receptor-expressing astrocytes. This contains RNAse-free perfusion, mixture of RNAscope and immunohistochemistry, and confocal imaging. This protocol supplies detailed details about tissue dealing with and a complete description of the RNAScope approach to label rat and mouse…

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Sensitive, Signal-Modulation Strategy for Discrimination of ECL Spectra and Investigation of Mutual Interactions of Emitters

The depth of electrochemiluminescence (ECL) often modifications quickly with the progress of the electrochemical course of, making it tough to find out the ECL spectrum with a traditional photomultiplier in a wavelength scan mannequin. Herein, a band-pass filter (BPF)-involved modulating technique is proposed to improve a traditional ECL analyzer to a extremely delicate ECL spectrometer…

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