Tag: recombinant proteins biotechnology
What’s the norm in normalization? A frightening note on the use of RT-qPCR in the livestock science
Reverse-Transcription quantitative PCR (RT-qPCR) offers a precious device to check gene expression with beautiful sensitivity. To retain its inferential energy, user-introduced technical variability have to be decreased and accounted for. Deciding on a set of stably expressed inside management genes (ICG), validated for every experimental situation/pattern set, is broadly accepted as a dependable approach to…
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Establishment of a duplex real-time qPCR method for detection of Salmonella spp. and Serratia fonticola in fishmeal
Salmonella spp. is a high-risk bacterial pathogen that’s monitored in imported animal-derived feedstuffs. Serratia fonticola is the bacterial species most often confused with Salmonella spp. in conventional identification strategies primarily based on biochemical traits, that are time-consuming and labor-intensive, and thus unsuitable for each day inspection and quarantine work. On this examine, we established a…
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