Tag: pcrichard.com
A New Specific and Sensitive RT-qPCR Method Based on Splinted 5′ Ligation for the Quantitative Detection of RNA Species Shorter than microRNAs
Not too long ago, we found a brand new household of unusually brief RNAs mapping to five.8S ribosomal RNA (rRNA) and which we named dodecaRNAs (doRNAs), based on the variety of core nucleotides (12 nt) their members include. To verify these small RNA-sequencing (RNA-Seq) knowledge, validate the existence of the 2 overly ample doRNAs-the minimal…
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Establishment of a duplex real-time qPCR method for detection of Salmonella spp. and Serratia fonticola in fishmeal
Salmonella spp. is a high-risk bacterial pathogen that’s monitored in imported animal-derived feedstuffs. Serratia fonticola is the bacterial species most often confused with Salmonella spp. in conventional identification strategies primarily based on biochemical traits, that are time-consuming and labor-intensive, and thus unsuitable for each day inspection and quarantine work. On this examine, we established a…
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