Tag: pcra
Current Practices for Reference Gene Selection in RT-qPCR of Aspergillus: Outlook and Recommendations for the Future
Aspergillus is a genus of filamentous fungi with huge geographic and ecological distributions. Species inside this genus are clinically, agriculturally and biotechnologically related, resulting in growing curiosity in elucidating gene expression dynamics of key metabolic and physiological processes. Reverse-transcription quantitative Polymerase Chain Response (RT-qPCR) is a delicate and particular methodology of quantifying gene expression. A vital…
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Comparison between Whatman FTA Elute Cards and Conventional Swab for the Detection of Pathogenic Enteric Bacteria Using an RT-qPCR Assay
The emergence of outbreaks of foodborne sickness is carefully related to meals contamination attributable to varied enteric pathogens, resembling Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus. The management of enteric pathogens poses a problem attributable to the truth that these pathogens can persist for an extended time frame within the surroundings. The fast detection of pathogenic…
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Validation of the analytical performance of nine commercial RT-qPCR kits for SARS-CoV-2 detection using certified reference material
The continued coronavirus illness 2019 (COVID-19) pandemic has turn into a public well being emergency. Though many reverse-transcription PCR (RT-PCR) assays have been developed, their efficiency, particularly sensitivity evaluation, has been insufficiently examined. On this examine, a preliminary comparability of the analytical sensitivity of 9 RT-qPCR kits from completely different producers was first performed utilizing…
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A New Specific and Sensitive RT-qPCR Method Based on Splinted 5′ Ligation for the Quantitative Detection of RNA Species Shorter than microRNAs
Not too long ago, we found a brand new household of unusually brief RNAs mapping to five.8S ribosomal RNA (rRNA) and which we named dodecaRNAs (doRNAs), based on the variety of core nucleotides (12 nt) their members include. To verify these small RNA-sequencing (RNA-Seq) knowledge, validate the existence of the 2 overly ample doRNAs-the minimal…
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