Current Practices for Reference Gene Selection in RT-qPCR of Aspergillus: Outlook and Recommendations for the Future

• Christopher Miller

Aspergillus is a genus of filamentous fungi with huge geographic and ecological distributions. Species inside this genus are clinically, agriculturally and biotechnologically related, resulting in growing curiosity in elucidating gene expression dynamics of key metabolic and physiological processes. Reverse-transcription quantitative Polymerase Chain Response (RT-qPCR) is a delicate and particular methodology of quantifying gene expression. A vital…