Ultra Rainbow

SPHEROT Ultra Rainbow Fluorescent Particles

New movement cytometers, with fluorescent channels from the UV to Far Pink, and corresponding fluorescent conjugates
at the moment are accessible. In consequence, we now have developed the Extremely Rainbow Fluorescent Particles with enhanced UV
and Far Pink fluorescence depth. The Extremely Rainbow Fluorescent Particles include a single peak and are
designed for checking the optical alignment of any movement cytometer in all channels.

Spherotech Cat. No. URFP-30-2 Directions to be used:
A. Preparation of Particles
1. Vortex the particles vigorously
2. Add 2 to Four drops of particles to 1mL of sheath fluid or DI water. The inclusion of a small quantity of detergent (~0.01%) within the dilution buffer will improve the proportion of the singlet inhabitants.

B. Each day Alignment
To find out the optical alignment of the system carry out the next:
1. Set a stay gate for the singlet inhabitants on the FSC vs SSC histogram to exclude aggregates
2. Modify the Acquire and Excessive voltage in order that the imply channel variety of the height is in a predetermined place on every histogram of curiosity. The histograms on the earlier web page can be utilized as a information.
3. Accumulate 5000 occasions contained in the gate
4. Report the % CV and Excessive Voltage for all fluorescence channels of curiosity.
5. Use a pc program resembling Excel to generate the Levy Jennings graphs.

NOTE: If the values on any parameter exceed these of the day-to-day common or preset values, that are decided by not less than one months value of information, extra calibration or alignment procedures must be carried out in response to the instrument operation handbook.

 

Movement Cytometry Alignment Particles

Spherotech gives all kinds of particles for figuring out if a movement cytometer is correctly aligned and has a clear movement cell with no fluidic blockage. Utilizing SPHERO Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions may be measured to find out the alignment and performance of the movement cytometer. Additiona info may be discovered at STN-8: Calibration and Efficiency Monitoring of Movement Cytometer Utilizing SPHERO Calibration Particles.

 

Ultra Rainbow
Extremely Rainbow
SPHERO™ Extremely Rainbow Fluorescent Particles – A single inhabitants of Rainbow Fluorescent Particles with enhanced UV and Far Pink fluorescence depth
Catalog No.
Description
Focus
Nominal Dimension
Bundle Dimension
Worth
URFP-02-2
Extremely Rainbow Fluorescent Particles
1010/mL
0.1-0.Three µm
2 mL
$250.00
URFP-05-2
Extremely Rainbow Fluorescent Particles
107/mL
0.4-0.6 µm
2 mL
$250.00
URFP-10-5
Extremely Rainbow Fluorescent Particles
107/mL
1.0-1.Four µm
5 mL
$250.00
URFP-30-2
Extremely Rainbow Fluorescent Particles
107/mL
3.0-3.Four µm
2 mL
$250.00
URFP-30-20
Extremely Rainbow Fluorescent Particles
107/mL
3.0-3.Four µm
20 mL
$2000.00
URFP01-30-2KExtremely Rainbow Fluorescent Particles106/mL3.0-3.Four µm2×15 mL$355.00
URFP01-30-10OkExtremely Rainbow Fluorescent Particles106/mL3.0-3.Four µm10×3 mL$410.00
URFP-38-2
Extremely Rainbow Fluorescent Particles
107/mL
3.5-3.9 µm
2 mL
$255.00
URFP-38-10
Extremely Rainbow Fluorescent Particles
107/mL
3.5-3.9 µm
10 mL
$1000.00
URFP-38-5A
Extremely Rainbow Fluorescent Particles, Mid-Vary
107/mL
3.5-3.9 µm
5 mL
$565.00
URFP-100-2
Extremely Rainbow Fluorescent Particles
107/mL
8.1-12.Zero µm
2 mL
$285.00
URFP-150-2
Extremely Rainbow Fluorescent Particles
5×106/mL
13.0-17.9 µm
2 mL
$285.00
URFP-200-5
Extremely Rainbow Fluorescent Particles
1.0% w/v
18.0-24.9 µm
5 mL
$290.00
URFP-300-5
Extremely Rainbow Fluorescent Particles
1.0% w/v
25.0-35.Zero µm
5 mL
$290.00

 

Description
Extremely Rainbow Fluorescent Particles (by Spherotech™) are internally labeled with a number of fluorophores. They exhibit full spectrum fluorescence, together with enhanced UV and Far Pink fluorescence. The combination of fluorophores allows Extremely Rainbow particles to be excited at any wavelength from 365 to 650nm. These particles can be found in two diameters, ~3.8µm and ~10.2µm.

Traits
Imply Diameter: ~3.8µm (3.61-3.99µm) or ~10.2µm (8.1 – 12.0µm); see your Certificates of Evaluation for precise measurement.
Focus: ~2 x 106particles/mL

Storage Buffer: Deionized water with 0.02% sodium azide and 0.01% NP-4O
Process
A dilution of 1-2 drops to 1mL will present an enough variety of particles for movement cytometry use.

 

Notes: Shake vigorously or vortex briefly earlier than use. Inclusion of a small quantity of detergent within the diluent will assist improve the variety of singlets. Diluted particles may be saved within the fridge for future use.

TaqProbe 2X qPCR MasterMix-Low ROX

MasterMix-PL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

Bio-Star Mastermix (2X) Low-ROX

P150 100 rcs (2,5 ml)
EUR 122.4

KiloGreen 2X qPCR MasterMix-Low ROX

MasterMix-KL 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

BrightGreen 5X qPCR MasterMix-Low ROX

MasterMix-5L 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 255.6

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 451.2

BrightGreen miRNA qPCR MasterMix-Low ROX

MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 181.2

BrightGreen Express 2X qPCR MasterMix-Low ROX

MasterMix-EL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

Green-2-Go 1-Step qRT-PCR Mastermix-iCycler

QPCR011-iC 100Rxn
EUR 287.08

Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn)

QPCR002-L 4X1.25ml
EUR 303.25

Bio-Star Mastermix with SYBR (2X) Low-ROX

Y250 100 rcs (2,5 ml)
EUR 122.4

Green-2-Go 1-Step qRT-PCR Mastermix-No Dye (100X20ul Rxn)

QPCR007-NODYE 100Rxn
EUR 287.08

Kodaq 2X PCR MasterMix

G497 5.0 ml (200 Rxns)
EUR 189.6

BlasTaq 2X PCR MasterMix

E36G895 800 rxn (10.0 ml)
EUR 342.86

2X PCR Taq MasterMix

G013 5.0 ml (200 Rxns)
EUR 104.4

2X PCR Taq MasterMix

G900 25.0 ml (1000 Rxns)
EUR 246

Real-Time PCR Mastermix 2x, Low Level of Rox , 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400LR each
EUR 261.6

Bloodirect 2X PCR MasterMix

G465 5.0 ml (200 Rxns)
EUR 166.8

2X PCR TaqFast MasterMix

G280 5.0 ml (200 Rxns)
EUR 160.8

2X PCR HotStart MasterMix

G906 5.0 ml (200 Rxns)
EUR 189.6

Green-2-Go 1-Step TaqProbe qRT-PCR Mastermix-no Dye (100X20ul Rxn)

QPCR008-NODYE 100Rxn
EUR 341.88