pureexo exosome isolation kit

pureexo exosome isolation kit

Cat. No. BL-P100 PureExo Cell
Producer 101Bio
Key Options
  • Straightforward to make use of: No ultra-centrifugation (< 2 hours)
  • 10 fold greater yield (vs. different kits and ultracentrifuge)
  • Save value (vs. antibodies-beads methodology)
  • Isolate Pure exosome (>95%)
  • Intact exosome (good morphology)
  • Use as little as 2 mL cell media to realize excessive yield of exosomes for any downstream purposes: EM research, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel evaluation of exosomal proteins
Particulars Product Dimension of 10 reactions (2 mL~Four mL medium / response). The yield of every response is 50~200 µL exosome, from which 150~400 µg exosomal protein or 50 ~ 200 ng exosomal RNA will be extracted.
Notes Storage: hold all bottles upright , in cool and darkish place. Shelf Life: 12 months. As a result of the exosome yield of this package is 10 fold greater than all different kits, you can begin with solely 2 mL medium. The utmost medium quantity of every response is Four mL from at most 5 x 105 cells. One PureExo® Column is just for one response. Don’t exceed Four mL medium or 5 x 105 cells to keep away from vague layer separation and column clogging.
Storage Temp Ambient Temperature
Delivery Situations Ambient
FAQ 1 May your package isolate/purify bigger exosome-like vesicles as much as 1000 nm in diameter, from tradition medium?
A: No, our package can not isolate vesicles greater than 300nm.2, Does your P101 package work for plasma?
A: It really works completely for plasma.Three What’s the minimal quantity of serum pattern will be processed by your PureExo package (Cat.#: P101)?
A: 50 ul.

Four Which package ought to I exploit for my urine and plasma samples?
A: Use DiagExo® package (Cat.#: P120) to isolate exosome from urine and Use PureExo® package for serum (Cat.#: P101) to isolate exosome from serum.

5, Can I exploit your package to isolate exosome or endothelial microparticles from cultured cells and from plasma?
A: Our P100 package can be utilized to isolate exosome from both cell tradition medium or serum. It’s not for cells.

6 Is your exosome prep contaminated with IgG and/or beads?
A: We use particular reagents to seize exosomes. We didn’t use antibody or beads for the isolation. So the ultimate exosome prep just isn’t contaminated with synthetic IgG or beads.

7, Do your exosome prep incorporates Albumin?
A: Some kinds of cells akin to hepatocytes carries enriched Albumin, and these liver derived exosomes will be launched into the circulation system. So, if the exosomes from these sorts of samples are remoted, it might incorporates Albumin.

8, Can I exploit your PureExo package (Cat. #P100) on our D.discoideum amoeba?
A: PureExo exosome isolation package captures launched exosomes or microvesicles from cells. For amoeba exosome isolation, we recommend to gather amoeba tradition medium as the identical strategy to gather cell tradition medium.

9, What’s the composition of the buffer that the ultimate exosomes prep can be in? Will it affect my downstream assay?
A: The ultimate remoted exosomes are aggregated nano-particles blended with a really small quantity of authentic tradition medium or serum. PureExo Exosome Isolation package gives intact exosomes which permits for a types of downstream purposes together with exosome floor labeling10, How have you learnt that an exosome prep is pure, freed from different membrane-derived microparticles
(shredding vesicles)?
A: The remoted microvesicle from cell tradition medium or serum utilizing our isolation kits confirmed sphere membrane encapsulated particles with the diameters various between 20-200nm beneath EM scanning. These characters assist to find out that the harvested microvesicle are exosomes. Shedding particles are reported to be irregular form with diameter as much as 1000nm. As well as, the dimensions distribution sample is confirmed by dynamic gentle scattering indicating the remoted microvesicles are of diameter between 100 and 250nm.

11, Are exosome functionally energetic?
A: Our collaborators used our kits to detect particular mirRNAs carried by exosomes. Luciferase exercise assay
confirmed energetic operate of exosomal microRNA after the exosomes had been utilized to cells transfected with focusing on mRNA 3’UTR vector.

12, Is it vital to make use of glass tubes?
A: Utilizing glass tubes as an alternative of plastic tubes is required in step Three and step 4.
Please discover glass tubes from FisherScientific. http://www.fishersci.com (Seek for Fisherbrand* Disposable
Borosilicate Glass Tubes Cat. NO: 14-961-26).

13, Why can’t I see clear fluff center layer?
A: The next dealing with errors could result in the failure:
1). Resolution A/B/C didn’t combine fully earlier than including the cell tradition medium or serum.
2). Didn’t carry out a low velocity centrifugation to take away cells and cell particles.
3). The ratio is off. For instance: 0.125 mL A + 0.125 mL B + 0.5mL C to 2 mL medium. Overproportion of serum/medium quantity to Resolution combination (A/B/C) will result in an indistinctive interphase.
4). The utmost beginning quantity is Four ml medium, and it must be from half million cultured cells or much less. If cell quantity or medium quantity exceed this quantity, the pattern must be splits into a number of samples to proceed.
5). Cultured cells weren’t starve for 48 hours to take away contaminated exosomes derived from FBS.

14, What biomarker(s) do you employ to determine / characterize the remoted exosomes?
A: We use exosome markers akin to flotillin, CD9, CD63, Alix, and TSG101 and many others to characterize the remoted exosomes.

15, What’s the yield of exosome utilizing your kits?
A: For P100 package (PureExo package for cell tradition medium), usually, 50 ~ 100 ul exosomes will be remoted from 2ml cell tradition media (As much as Four ml of medium will be processed per response). If all of the harvested exosomes are used for exosomal protein extraction (utilizing our P200 package), the entire protein yield ranges from 150 ~ 200 ug / 2mL medium, 300 ~ 400 ug / Four ml medium. If all of the remoted exosome are used for RNA extraction (utilizing our P200 package), whole exosomal RNA yield is 50 ~ 100 ng / 2ml medium, 100 ~ 200 ng / 4ml medium. For P101 package (PureExo package for serum or plasma), usually 100 ~ 200 ul exosome will be remoted from 100ul to 600ul serum. If all of the harvested exosomes are used for exosomal protein extraction (utilizing our P200 package), the entire exosomal protein yield ranges from 300-400 ug protein. If all of the remoted exosome are used for RNA extraction (utilizing our P200 package), whole exosomal RNA yield is 100 ~ 300ng exosomal RNA For P120 package (DiagExo package for Urine), usually 50 ~ 200 ul exosome will be remoted from Three mL urine. If all of the harvested exosomes are used for exosomal protein extraction (utilizing our P200 package), the entire exosomal protein yield ranges from 150-400ug protein. If all of the remoted exosome are used for RNA extraction (utilizing our P200 package), whole exosomal RNA yield is 50 ~ 200ng exosomal RNA

16, Can I exploit heparin or EDTA tube to gather blood pattern if I must isolate exosome from plasma?
A: No. Heparin and EDTA will considerably impair the downstream assays. Use No-heparin-No-EDTA tube to gather the blood pattern. Centrifuge the pattern instantly to gather the plasma for exosome isolation. .

pureexo exosome isolation kit
pureexo exosome isolation package

Software

Isolate pure exosome from cell tradition media (supernatant). This package yields a lot greater purity than exosome precipitation methodology. This product is for analysis use solely.

 

Description

 

P100 package is for exosome purification / isolation at excessive yield and purity from cell tradition media.

  • Straightforward to make use of: No ultra-centrifugation (< 2 hours)
  • 10 fold greater yield (vs. different kits and ultracentrifuge)
  • Save value (vs. antibodies-beads methodology)
  • Isolate Pure exosome (>95% purity vs. 25% purity by exosome precipitation methodology)
  • Intact exosome (good morphology).

 

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