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Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples
Utility of Repeat Nasopharyngeal SARS-CoV-2 RT-PCR Testing and Refinement of Diagnostic Stewardship Strategies at a Tertiary Care Tutorial Center in a Low-Prevalence Area of america
Background: Numerous parts have led to a very extreme amount of maximum acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription polymerase chain response (RT-PCR) testing. Concerns exist about sensitivity and false-negative SARS-CoV-2 RT-PCR testing outcomes. We describe a retrospective observational analysis inspecting the utility of repeat nasopharyngeal (NP) SARS-CoV-2 RT-PCR testing at a tutorial coronary heart in a low-prevalence setting.
Methods: All victims inside our nicely being system with >1 NP SARS-CoV-2 RT-PCR test end result have been included. SARS-CoV-2 RT-PCR testing was carried out primarily based on 1 of 4 validated assays. Key scientific and demographic data have been collected, along with whether or not or not the affected particular person was inpatient or outpatient at time of the test and whether or not or not the test was carried out as part of a person beneath investigation (PUI) for potential coronavirus sickness 2019 or for asymptomatic screening.
Outcomes: A whole of 660 victims had >1 NP SARS-CoV-2 PCR test carried out. The preliminary test was unfavourable in 638. There have been solely 6 negative-to-positive conversions (0.9%). All 6 have been outpatients current course of a PUI workup 5-17 days after an preliminary unfavourable end result. In >260 inpatients with repeat testing, we found no instances of negative-to-positive conversion along with these current course of PUI or asymptomatic evaluation.
Conclusions: In a low-prevalence house, repeat inpatient testing after an preliminary unfavourable end result, using a extraordinarily analytically delicate SARS-CoV-2 RT-PCR, did not present negative-to-positive conversion. The scientific sensitivity of NP RT-PCR testing is also larger than beforehand believed. These outcomes have helped type diagnostic stewardship pointers, notably steering to decrease repeated testing inside the inpatient setting to optimize test utilization and defend belongings.
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Enchancment of a real-time PCR assay for detection and quantification of Streptococcus iniae using the lactate permease gene
The aim of this analysis is the occasion and evaluation of a quick and proper quantitative PCR (qPCR)-based protocol for detection of zoonotic pathogen Streptococcus iniae in bacterial cultures and tissues of diseased fish. For this aim, the lactate permease-encoding (lldY) gene was chosen as a aim for the design of S. iniae-specific primers based on comparative genomic analysis using 45 sequences retrieved from NCBI genome database. Specificity and applicability of these primers have been examined using 115 bacterial strains and fish tissues contaminated with S. iniae.
Sensitivity, reproducibility and effectivity of qPCR assay have been moreover determined. The developed qPCR assay confirmed 100% specificity with pure bacterial cultures or DNA extracted from S. iniae or tissues of fish contaminated with the bacterium. The technique has extreme sensitivity with a detection prohibit of 1.12 × 101 amplicon copies per assay (equal to 2 × 10-9 ng/µl) using bacterial DNA and of 1.44 × 101 gene copies in tissues of fish contaminated with S. iniae. In conclusion, this qPCR protocol provides an right and delicate numerous for the identification of S. iniae and its detection on fish tissues which may be carried out as a routine instrument in microbiological laboratories.
Enchancment of a New Multiplex Precise Time RT-PCR Assay for SARS-CoV-2 Detection
We describe the occasion of a model new multiplex precise time reverse transcription (RT)-PCR test for detection of SARS-CoV-2, with primers designed to amplify a 108 bp aim on the spike flooring glycoprotein (S gene) and a hydrolysis Taqman probe designed to notably detect SARS-CoV-2. We then evaluated the prohibit of detection (LOD) and scientific effectivity of this new assay. A LOD analysis with inactivated virus exhibited equal effectivity to the modified CDC assay with a closing LOD of 1,301 ± 13 genome equivalents/ml for the Northwell Nicely being Laboratories laboratory developed test (NWHL LDT) vs. 1,249 ± 14 genome equivalents/ml for the modified CDC assay.
Furthermore, a scientific evaluation with 270 nasopharyngeal (NP) swab specimens exhibited 98.5% optimistic % settlement and 99.3% unfavourable % settlement compared with the modified CDC assay. The NWHL LDT multiplex design permits testing of 91 victims per plate, versus a most of 29 victims per plate on the modified CDC assay, providing the benefit of testing significantly additional victims per run and saving reagents, all through a time when every of these parameters are essential.
The outcomes present that the NWHL LDT multiplex assay performs along with the modified CDC assay, nevertheless is additional surroundings pleasant and worth environment friendly and may be utilized as a diagnostic assay and for epidemiological surveillance and scientific administration of SARS-CoV-2.
![]() GPR160 Antibody | |||
E047605 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
![]() GPR160 Antibody | |||
E11-140G | EnoGene | 100μg | EUR 225 |
Description: Available in various conjugation types. |
![]() GPR160 Antibody | |||
E19-4899 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
![]() GPR160 Antibody | |||
DF4899 | Affbiotech | 200ul | EUR 420 |
![]() GPR160 Antibody | |||
DF4899-100ul | Affinity Biosciences | 100ul | EUR 280 |
![]() GPR160 Antibody | |||
DF4899-200ul | Affinity Biosciences | 200ul | EUR 350 |
![]() GPR160 antibody | |||
70R-31407 | Fitzgerald | 100 ug | EUR 294 |
Description: Rabbit polyclonal GPR160 antibody |
![]() GPR160 antibody | |||
70R-50939 | Fitzgerald | 100 ul | EUR 242 |
Description: Purified Polyclonal GPR160 antibody |
![]() GPR160 Antibody | |||
1-CSB-PA008825 | Cusabio |
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Description: A polyclonal antibody against GPR160. Recognizes GPR160 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000 |
![]() GPR160 Antibody | |||
ABD4899 | Nova Lifetech | 100ug | EUR 325 |
![]() GPR160 Antibody | |||
MBS7119398-005mg | MyBiosource | 0.05mg | EUR 150 |
![]() GPR160 Antibody | |||
MBS7119398-01mg | MyBiosource | 0.1mg | EUR 190 |
![]() GPR160 Antibody | |||
MBS7119398-5x01mg | MyBiosource | 5x0.1mg | EUR 845 |
![]() GPR160 Antibody | |||
MBS9232645-01mL | MyBiosource | 0.1mL | EUR 415 |
![]() GPR160 Antibody | |||
MBS9232645-5x01mL | MyBiosource | 5x0.1mL | EUR 1841 |
![]() GPR160 Antibody | |||
MBS8515601-01mg | MyBiosource | 0.1mg | EUR 305 |
![]() GPR160 Antibody | |||
MBS8515601-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 465 |
![]() GPR160 Antibody | |||
MBS8515601-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 465 |
![]() GPR160 Antibody | |||
MBS8515601-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 465 |
![]() GPR160 Antibody | |||
MBS8515601-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 465 |
![]() GPR160 Antibody | |||
MBS853183-01mg | MyBiosource | 0.1mg | EUR 305 |
![]() GPR160 Antibody | |||
MBS853183-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 465 |
![]() GPR160 Antibody | |||
MBS853183-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 465 |
![]() GPR160 Antibody | |||
MBS853183-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 465 |
![]() GPR160 Antibody | |||
MBS853183-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 465 |
![]() GPR160 Antibody | |||
MBS5315557-01mL | MyBiosource | 0.1mL | EUR 470 |
![]() GPR160 Antibody | |||
MBS5315557-5x01mL | MyBiosource | 5x0.1mL | EUR 1955 |
![]() GPR160 antibody | |||
MBS535434-005mg | MyBiosource | 0.05mg | EUR 1125 |
![]() GPR160 antibody | |||
MBS535434-5x005mg | MyBiosource | 5x0.05mg | EUR 4905 |
![]() GPR160 Antibody | |||
MBS9606351-01mL | MyBiosource | 0.1mL | EUR 260 |
![]() GPR160 Antibody | |||
MBS9606351-02mL | MyBiosource | 0.2mL | EUR 305 |
![]() GPR160 Antibody | |||
MBS9606351-5x02mL | MyBiosource | 5x0.2mL | EUR 1220 |
![]() GPR160 Antibody | |||
MBS9430587-01mL | MyBiosource | 0.1mL | EUR 305 |
![]() GPR160 Antibody | |||
MBS9430587-5x01mL | MyBiosource | 5x0.1mL | EUR 1230 |
![]() GPR160 Antibody | |||
MBS9507949-005mL | MyBiosource | 0.05mL | EUR 285 |
![]() GPR160 Antibody | |||
MBS9507949-01mL | MyBiosource | 0.1mL | EUR 385 |
![]() GPR160 Antibody | |||
MBS9507949-5x01mL | MyBiosource | 5x0.1mL | EUR 1590 |
![]() GPR160 Antibody | |||
MBS9509528-005mL | MyBiosource | 0.05mL | EUR 285 |
![]() GPR160 Antibody | |||
MBS9509528-01mL | MyBiosource | 0.1mL | EUR 385 |
![]() GPR160 Antibody | |||
MBS9509528-5x01mL | MyBiosource | 5x0.1mL | EUR 1590 |
![]() GPR160 Antibody | |||
G140-100ul | Assay Biotech | 100μl | EUR 217 |
Description: GPR160 Rabbit Polyclonal Antibody |
![]() GPR160 Antibody | |||
G140-50ul | Assay Biotech | 50μl | EUR 143.5 |
Description: GPR160 Rabbit Polyclonal Antibody |
![]() GPR160 Polyclonal Antibody | |||
ABP54526-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350 |
![]() GPR160 Polyclonal Antibody | |||
ABP54526-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350 |
![]() GPR160 Polyclonal Antibody | |||
ABP54526-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350 |
![]() GPR160 Polyclonal Antibody | |||
E20-71990 | EnoGene | 100ug | EUR 225 |
Description: Available in various conjugation types. |
![]() GPR160 Conjugated Antibody | |||
C47605 | SAB | 100ul | EUR 476.4 |
![]() GPR160 Polyclonal Antibody | |||
E-AB-19949-120uL | Elabscience Biotech | 120uL | EUR 240 |
Description: Unconjugated |
![]() GPR160 Polyclonal Antibody | |||
E-AB-19949-200uL | Elabscience Biotech | 200uL | EUR 399 |
Description: Unconjugated |
![]() GPR160 Polyclonal Antibody | |||
E-AB-19949-20uL | Elabscience Biotech | 20uL | EUR 73 |
Description: Unconjugated |
![]() GPR160 Polyclonal Antibody | |||
E-AB-19949-60uL | Elabscience Biotech | 60uL | EUR 143 |
Description: Unconjugated |
![]() GPR160 Polyclonal Antibody | |||
BT-AP03712-100ul | Jiaxing Korain Biotech Ltd (BT Labs) | 100ul | Ask for price |
Description: Orphan receptor. |
![]() GPR160 Polyclonal Antibody | |||
BT-AP03712-20ul | Jiaxing Korain Biotech Ltd (BT Labs) | 20ul | Ask for price |
Description: Orphan receptor. |
![]() GPR160 Polyclonal Antibody | |||
BT-AP03712-50ul | Jiaxing Korain Biotech Ltd (BT Labs) | 50ul | Ask for price |
Description: Orphan receptor. |
![]() GPR160 Polyclonal Antibody | |||
E44H04883 | EnoGene | 100ul | EUR 255 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
![]() GPR160 Polyclonal Antibody | |||
MBS8522971-01mg | MyBiosource | 0.1mg | EUR 305 |
![]() GPR160 Polyclonal Antibody | |||
MBS8522971-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 465 |
![]() GPR160 Polyclonal Antibody | |||
MBS8522971-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 465 |
![]() GPR160 Polyclonal Antibody | |||
MBS8522971-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 465 |
![]() GPR160 Polyclonal Antibody | |||
MBS8522971-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 465 |
![]() GPR160 Polyclonal Antibody | |||
MBS2561838-002mL | MyBiosource | 0.02mL | EUR 135 |
![]() GPR160 Polyclonal Antibody | |||
MBS2561838-006mL | MyBiosource | 0.06mL | EUR 190 |
![]() GPR160 Polyclonal Antibody | |||
MBS2561838-012mL | MyBiosource | 0.12mL | EUR 265 |
![]() GPR160 Polyclonal Antibody | |||
MBS2561838-02mL | MyBiosource | 0.2mL | EUR 415 |
![]() GPR160 Polyclonal Antibody | |||
MBS2561838-5x02mL | MyBiosource | 5x0.2mL | EUR 1835 |
![]() GPR160 Conjugated Antibody | |||
MBS9455452-01mLAF350 | MyBiosource | 0.1mL(AF350) | EUR 480 |
![]() GPR160 Conjugated Antibody | |||
MBS9455452-01mLAF405 | MyBiosource | 0.1mL(AF405) | EUR 480 |
![]() GPR160 Conjugated Antibody | |||
MBS9455452-01mLAF488 | MyBiosource | 0.1mL(AF488) | EUR 480 |
![]() GPR160 Conjugated Antibody | |||
MBS9455452-01mLAF555 | MyBiosource | 0.1mL(AF555) | EUR 480 |
![]() GPR160 Conjugated Antibody | |||
MBS9455452-01mLBiotin | MyBiosource | 0.1mL(Biotin) | EUR 480 |
![]() GPR160 Polyclonal Antibody | |||
RD219949A-120uL | Reddot Biotech | 120μL | EUR 360 |
Description: Orphan receptor. |
![]() GPR160 Polyclonal Antibody | |||
RD219949A-200uL | Reddot Biotech | 200μL | EUR 630 |
Description: Orphan receptor. |
![]() GPR160 Polyclonal Antibody | |||
RD219949A-20uL | Reddot Biotech | 20μL | EUR 109.5 |
Description: Orphan receptor. |
![]() GPR160 Polyclonal Antibody | |||
RD219949A-60uL | Reddot Biotech | 60μL | EUR 214.5 |
Description: Orphan receptor. |
![]() GPR160 Polyclonal Antibody | |||
UB-GEN-13108 | UpingBio | 100 ul | EUR 200 |
![]() GPR160 Antibody (C-term) | |||
MBS9213578-008mL | MyBiosource | 0.08mL | EUR 210 |
![]() GPR160 Antibody (C-term) | |||
MBS9213578-04mL | MyBiosource | 0.4mL | EUR 430 |
![]() GPR160 Antibody (C-term) | |||
MBS9213578-5x04mL | MyBiosource | 5x0.4mL | EUR 1910 |
![]() Polyclonal GPR160 Antibody (Internal) | |||
APR16513G | Leading Biology | 0.05ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 (Internal). This antibody is tested and proven to work in the following applications: |
![]() GPR160 Rabbit Polyclonal Antibody | |||
ES5525-100ul | ELK Biotech | 100ul | EUR 124 |
Description: A Rabbit Polyclonal antibody against GPR160 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
![]() GPR160 Rabbit Polyclonal Antibody | |||
ES5525-50ul | ELK Biotech | 50ul | EUR 74 |
Description: A Rabbit Polyclonal antibody against GPR160 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
![]() GPR160 Rabbit Polyclonal Antibody | |||
E10G13515 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
![]() GPR160 Rabbit Polyclonal Antibody | |||
E10G06629 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
![]() GPR160 Antibody - C-terminal region | |||
MBS3217022-01mL | MyBiosource | 0.1mL | EUR 455 |
![]() GPR160 Antibody - C-terminal region | |||
MBS3217022-5x01mL | MyBiosource | 5x0.1mL | EUR 1995 |
![]() GPR160 Antibody - N-terminal region | |||
MBS3217023-01mL | MyBiosource | 0.1mL | EUR 455 |
![]() GPR160 Antibody - N-terminal region | |||
MBS3217023-5x01mL | MyBiosource | 5x0.1mL | EUR 1995 |
![]() Polyclonal GPR160 Antibody (Extracellular Domain) | |||
APR16512G | Leading Biology | 0.05mg | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
![]() Anti- G Protein-Coupled Receptor GPR160/GPCR150 Human Antibody | |||
GWB-84AB9F | GenWay Biotech | 0.05 mg | Ask for price |
![]() GPR160 Antibody (C-term) Blocking Peptide | |||
MBS9228244-INQUIRE | MyBiosource | INQUIRE | Ask for price |