A novel multiplex qPCR method for assessing the comparative lengths of telomeres
Background: The comparative size of telomeres is taken into account to be associated to ailments corresponding to most cancers, growing older, and cardiovascular ailments. qPCR is presently one of many primary strategies for detecting telomere size. Nevertheless, because of the distinctive sequence of telomeres (extremely repetitive six-base sequence), it’s tough to design primers and probes to…
Read MoreAuthor Correction: A simple and robust real-time qPCR method for the detection of PIK3CA mutations
Background: Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has precipitated a pandemic of pneumonia spreading all over the world, resulting in critical threats to public well being and attracting monumental consideration. There’s an pressing want for delicate diagnostic testing implementation to regulate and handle SARS-CoV-2 in public well being laboratories. The quantitative reverse transcription PCR (RT-qPCR) assay is…
Read MoreValidation of fetal microchimerism after pregnancy in the ovine using qPCR
Fetal microchimerism has been detected in maternal tissues of people and rodents throughout and after being pregnant. Research specializing in fetal DNA switch to maternal tissues in home animals are restricted, particularly in sheep. Fetal ram DNA was noticed within the maternal circulation throughout being pregnant, however it isn’t recognized if this chimerism persists in…
Read MoreUltra-sensitive AAV capsid detection by immunocapture-based qPCR following factor VIII gene transfer
Adeno-associated virus (AAV)-based gene remedy vectors are replication-incompetent and thus pose minimal threat for horizontal transmission or launch into the surroundings. In research with AAV5-FVIII-SQ (valoctocogene roxaparvovec), an investigational gene remedy for hemophilia A, residual vector DNA was detectable in blood, secreta, and excreta, nevertheless it remained unclear how lengthy structurally intact AAV5 vector capsids…
Read MoreRT-qPCR Diagnostics: The “Drosten” SARS-CoV-2 Assay Paradigm
The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an established tool for the diagnosis of RNA pathogens. Its potential for automation has caused it to be used as a presence/absence diagnostic tool even when RNA quantification is not required. This technology has been pushed to the forefront of public awareness by the COVID-19 pandemic,…
Read MoreA qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
White lupin (Lupinus albus) represents an vital legume crop in Europe and different elements of the world on account of its excessive protein content material and potential for low-input agriculture. Nonetheless, most cultivars are vulnerable to anthracnose attributable to Colletotrichum lupini, a seed- and air-borne fungal pathogen that causes extreme yield losses. The goal of this work…
Read MorePooled RT-qPCR testing for SARS-CoV-2 surveillance in schools – a cluster randomised trial
Background: The extent to which youngsters and adolescents contribute to SARS-CoV-2 transmission stays not absolutely understood. Novel high-capacity testing strategies might present real-time epidemiological knowledge in academic settings serving to to determine a rational method to forestall and decrease SARS-CoV-2 transmission. We investigated whether or not pooling of samples for SARS-CoV-2 detection by RT-qPCR is a…
Read MoreClinical performance and accuracy of a qPCR-based SARS-CoV-2 mass-screening workflow for healthcare-worker surveillance using pooled self-sampled gargling solutions: a cross-sectional study
Introduction: The large number of asymptomatic SARS-CoV-2 infections necessitates general screening of employees. We evaluate the performance of a SARS-CoV-2 screening program in asymptomatic healthcare-workers (HCW), utilizing self-sampled gargling-solution and sample pooling for RT-qPCR. Methods: We conducted a cross-sectional retrospective study to collect real-life data on the performance of a screening-workflow based on automated-pooling and high-throughput qPCR…
Read MoreMitochondrial loci enable specific qPCR detection of the pathogen causing contemporary impatiens downy mildew epidemics
Impatiens downy mildew (IDM) illness is a main constraint on the manufacturing of Impatiens walleriana, a preferred and economically vital floriculture plant. IDM is attributable to the biotrophic oomycete Plasmopara destructor that emerged as a pathogen of I. walleriana within the 2000s. To allow P. destructor detection and quantification, a hydrolysis probe-based quantitative PCR diagnostic assay was developed based mostly on distinctive…
Read MorePlatinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples
Isolation, contact tracing and restrictions on social motion are being globally carried out to stop and management onward unfold of SARS-CoV-2, though the an infection threat modelled on RNA detection by RT-qPCR stays biased as viral shedding and infectivity will not be discerned. Thus, we aimed to develop a speedy viability RT-qPCR process to deduce…
Read More