Identification and validation of reference genes for RT-qPCR analysis in fetal rat pancreas
A SYBR inexperienced I real-time polymerase chain response (PCR) assay for detection and quantification of Trichomonas gallinae
Trichomonas gallinae are parasitic flagellates of significance in wild and residential birds. The parasite is worldwide distributed, and Columbine birds are its foremost host. Current evaluation focuses completely on epidemiological and phylogenetic analysis. Nonetheless, there could also be nonetheless a lack of expertise regarding parasite-host interaction or treatment development.
Precise-time PCR is a helpful gizmo for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp space of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed.
A daily curve was prepared for quantification analysis. Assay effectivity, linearity, and dissociation analysis have been effectively carried out. Specificity, sensibility, and reproducibility analysis have been examined. This assay is perhaps a helpful gizmo not only for diagnostic features however moreover for future in vivo and in vitro T. gallinae analysis.
Enchancment of a standard RT-PCR assay for grapevine vitiviruses
The genus Vitivirus throughout the family Betaflexiviridae comprises eleven viruses acknowledged to infect grapevine: grapevine vitiviruses A, B, D, E, F, G, H, I, J, L and M (GVA-GVM). Three of these viruses, GVA, GVB and GVD, have been associated to the etiology of rugose picket sickness in grapevine and set off agronomically important losses. The alternative vitiviruses have been further simply these days discovered and their outcomes on grapevine are undetermined. To certify grape supplies for propagation as virus examined, an updated reverse transcription PCR (RT-PCR) assay to detect all acknowledged vitiviruses is fascinating.
To carry out this, a lot of grapevine vitivirus sequences have been aligned on the amino acid diploma to hunt for conserved motifs. Two extraordinarily conserved motifs have been found at a superb distance for RT-PCR detection throughout the RNA-dependent RNA polymerase space of the replicase protein. The amino acid motifs have been once more translated to create degenerate primers and used to effectively amplify all eleven grapevine vitiviruses. The RT-PCR primers have been used to verify a panel of vitivirus-infected vines for inclusivity along with vines contaminated with fastidiously related viruses throughout the Betaflexiviridae family (i.e. grapevi
ne pinot gris virus and grapevine rupestris stem pitting-associated virus) for exclusivity. Broader use of these primers to detect vitiviruses in several plant hosts was investigated. In summary, an end-point RT-PCR assay that detects all the acknowledged grapevine vitiviruses and possibly totally different members of the genus Vitivirus has been developed. The frequent assay represents another choice to explicit individual assays to reduce the work associated to the prognosis of vitiviruses, along with for regulatory features.
Pathologic full response (pCR) costs and outcomes after neoadjuvantchemoradiotherapy with proton or photon radiation for adenocarcinomas of the esophagus and gastroesophageal junction
Background: Pathologic full response (pCR) after neoadjuvantchemoradiotherapy (nCRT) is expounded to improved survival in victims dealt with for esophageal most cancers. Whereas proton beam treatment (PBT) has been demonstrated to reduce toxicities with nCRT, no info evaluating pCR costs between modalities exist to date. We investigated pCR costs in victims with distal esophageal/GEJ adenocarcinomas current course of trimodality treatment with nCRT-PBT or photon-based nCRT with the hypothesis that pathologic responses with PBT could be a minimal of as extreme as with photon treatment.
Methods: A single-institutional consider of victims with distal esophageal adenocarcinoma dealt with with trimodality treatment from 2015-2018 using PBT was achieved. PBT victims have been matched 1:2 to victims dealt with with photons. Chi sq. and two-sample t-tests have been utilized to verify traits, and the Kaplan Meier approach was used to estimate oncologic endpoints.
Outcomes: Eighteen consecutive PBT victims have been acknowledged and as compared with 36 photon victims. All victims obtained concurrent chemotherapy; 98% with carboplatin/paclitaxel. Most victims have been male (91%) and White (89%); median age was 62 years (range, 31-76 years). Median radiation dose in every cohorts was 50.4 Gy (range, 41.4-50.4 Gy); all applications have been delivered in 1.8Gy fractions. Age, gender and race have been successfully balanced.
Victims dealt with with PBT had a significantly better pre-treatment nodal stage (N) and AJCC 7th model stage grouping (P=0.02, P=0.03). No matter this, tumoral and nodal clearance and pCR costs have been equal between cohorts (P=0.66, P=0.11, P=0.63, respectively). Complete pCR and explicit individual principal and nodal clearance costs, complete survival (OS), locoregional administration (LRC), and distant metastatic administration did not significantly differ between modalities (all P>0.05). Primary perioperative events have been balanced; however, there have been 5 (14%) perioperative deaths throughout the photon cohort as compared with 0 (0%) throughout the proton cohort (P=0.06).
Conclusions: Utilizing PBT in trimodality treatment for distal esophageal adenocarcinoma yields pCR costs just like photon radiation and historic controls. Pathologic responses and oncologic outcomes on this analysis did not differ significantly between modalities no matter PBT victims having better AJCC ranges and nodal sickness burdens.
 GPR12 antibody |
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| 70R-30940 | Fitzgerald | 100 ug | EUR 392.4 |
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Description: Rabbit polyclonal GPR12 antibody |
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GPR12 antibody |
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| 20R-GR041 | Fitzgerald | 50 ug | EUR 787.2 |
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Description: Rabbit polyclonal GPR12 antibody |
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 GPR12 Antibody |
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| ABD2721 | Lifescience Market | 100 ug | EUR 525.6 |
GPR12 Antibody |
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| DF2721 | Affbiotech | 200ul | EUR 420 |
GPR12 Antibody |
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| 1-CSB-PA008905 | Cusabio |
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000 |
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GPR12 Antibody |
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| 1-CSB-PA009742LA01HU | Cusabio |
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
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 GPR12 |
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| RA30050 | Neuromics | 50 ug | EUR 741.6 |
 Gpr12/ Rat Gpr12 ELISA Kit |
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| ELI-09764r | Lifescience Market | 96 Tests | EUR 1063.2 |
 GPR12 Polyclonal Antibody |
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| ABP54586-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
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GPR12 Polyclonal Antibody |
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| ABP54586-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
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GPR12 Polyclonal Antibody |
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| ABP54586-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
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 GPR12 Conjugated Antibody |
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| C44910 | SAB | 100ul | EUR 476.4 |
GPR12 Polyclonal Antibody |
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| ES5585-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
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GPR12 Polyclonal Antibody |
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| ES5585-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
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 GPR12 siRNA |
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| 20-abx902268 | Abbexa |
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GPR12 siRNA |
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| 20-abx918427 | Abbexa |
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GPR12 siRNA |
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| 20-abx918428 | Abbexa |
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 GPR12 Antibody, HRP conjugated |
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| 1-CSB-PA009742LB01HU | Cusabio |
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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 GPR12 Antibody, FITC conjugated |
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| 1-CSB-PA009742LC01HU | Cusabio |
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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 GPR12 Antibody, Biotin conjugated |
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| 1-CSB-PA009742LD01HU | Cusabio |
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Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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 GPR12 Blocking Peptide |
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| 20-abx062679 | Abbexa |
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GPR12 Blocking Peptide |
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| DF2721-BP | Affbiotech | 1mg | EUR 234 |
) Polyclonal GPR12 Antibody (Extracellular Domain) |
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| APR16480G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
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) Polyclonal GPR12 Antibody (N-Terminus) |
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| APR16481G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications: |
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 Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
 Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
 Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
 Human GPR12 shRNA Plasmid |
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| 20-abx951900 | Abbexa |
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 Mouse GPR12 shRNA Plasmid |
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| 20-abx970621 | Abbexa |
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 Rat GPR12 shRNA Plasmid |
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| 20-abx986567 | Abbexa |
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 GPR12 ELISA KIT|Human |
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| EF005024 | Lifescience Market | 96 Tests | EUR 826.8 |
) GPR12 Recombinant Protein (Rat) |
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| RP203285 | ABM | 100 ug | Ask for price |
GPR12 Recombinant Protein (Rat) |
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| RP203288 | ABM | 100 ug | Ask for price |
) GPR12 Recombinant Protein (Human) |
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| RP061035 | ABM | 100 ug | Ask for price |
) GPR12 Recombinant Protein (Mouse) |
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| RP139421 | ABM | 100 ug | Ask for price |
GPR12 Recombinant Protein (Mouse) |
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| RP139424 | ABM | 100 ug | Ask for price |
 Polyclonal GPR12 Antibody - C-terminal region |
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| APR16482G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications: |
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 Antibody) G Protein Coupled Receptor 12 (GPR12) Antibody |
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| 20-abx147559 | Abbexa |
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G Protein Coupled Receptor 12 (GPR12) Antibody |
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| 20-abx009208 | Abbexa |
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G Protein Coupled Receptor 12 (GPR12) Antibody |
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| 20-abx338465 | Abbexa |
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G Protein Coupled Receptor 12 (GPR12) Antibody |
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| 20-abx324375 | Abbexa |
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 (pORF)) Gpr12 ORF Vector (Rat) (pORF) |
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| ORF067763 | ABM | 1.0 ug DNA | EUR 607.2 |
Gpr12 ORF Vector (Rat) (pORF) |
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| ORF067764 | ABM | 1.0 ug DNA | EUR 607.2 |
 (pORF)) GPR12 ORF Vector (Human) (pORF) |
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| ORF020346 | ABM | 1.0 ug DNA | EUR 486 |
 (pORF)) Gpr12 ORF Vector (Mouse) (pORF) |
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| ORF046475 | ABM | 1.0 ug DNA | EUR 607.2 |
Gpr12 ORF Vector (Mouse) (pORF) |
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| ORF046476 | ABM | 1.0 ug DNA | EUR 607.2 |
 Antibody (HRP)) G Protein Coupled Receptor 12 (GPR12) Antibody (HRP) |
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| 20-abx335850 | Abbexa |
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 Antibody (FITC)) G Protein Coupled Receptor 12 (GPR12) Antibody (FITC) |
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| 20-abx335851 | Abbexa |
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 Antibody (Biotin)) G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin) |
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| 20-abx335852 | Abbexa |
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) Gpr12 sgRNA CRISPR Lentivector set (Rat) |
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| K7010601 | ABM | 3 x 1.0 ug | EUR 406.8 |
) GPR12 sgRNA CRISPR Lentivector set (Human) |
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| K0889201 | ABM | 3 x 1.0 ug | EUR 406.8 |
) Gpr12 sgRNA CRISPR Lentivector set (Mouse) |
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| K3382801 | ABM | 3 x 1.0 ug | EUR 406.8 |
 (Target 1)) Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K7010602 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 2)) Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K7010603 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 3)) Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K7010604 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 1)) GPR12 sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K0889202 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 2)) GPR12 sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K0889203 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 3)) GPR12 sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K0889204 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 1)) Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K3382802 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 2)) Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K3382803 | ABM | 1.0 ug DNA | EUR 184.8 |
 (Target 3)) Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K3382804 | ABM | 1.0 ug DNA | EUR 184.8 |
 (pPB-C-His)) GPR12 Protein Vector (Human) (pPB-C-His) |
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| PV081381 | ABM | 500 ng | EUR 662.4 |
 (pPB-N-His)) GPR12 Protein Vector (Human) (pPB-N-His) |
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| PV081382 | ABM | 500 ng | EUR 662.4 |
 (pPM-C-HA)) GPR12 Protein Vector (Human) (pPM-C-HA) |
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| PV081383 | ABM | 500 ng | EUR 662.4 |
 (pPM-C-His)) GPR12 Protein Vector (Human) (pPM-C-His) |
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| PV081384 | ABM | 500 ng | EUR 662.4 |
 (pPB-C-His)) GPR12 Protein Vector (Mouse) (pPB-C-His) |
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| PV185898 | ABM | 500 ng | EUR 723.6 |
 (pPB-N-His)) GPR12 Protein Vector (Mouse) (pPB-N-His) |
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| PV185899 | ABM | 500 ng | EUR 723.6 |
 (pPM-C-HA)) GPR12 Protein Vector (Mouse) (pPM-C-HA) |
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| PV185900 | ABM | 500 ng | EUR 723.6 |
 (pPM-C-His)) GPR12 Protein Vector (Mouse) (pPM-C-His) |
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| PV185901 | ABM | 500 ng | EUR 723.6 |
GPR12 Protein Vector (Mouse) (pPB-C-His) |
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| PV185902 | ABM | 500 ng | EUR 723.6 |
