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epigentek methylflash methylated dna quantification kit

Product Overview

The MethylFlash Methylated DNA Quantification Package (Fluorometric) is a whole set of optimized buffers and reagents to fluorometrically quantify international DNA methylation by particularly measuring ranges of 5-methylcytosine (5-mC) in a microplate-based format. In comparison with LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash™ expertise straight quantifies precise international DNA methylation.

This equipment can also be particularly optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Package (Fluorometric) for concurrently quantifying each methylated DNA and hydroxymethylated DNA.

Product Options
The MethylFlash™ Methylated DNA Quantification Package (Fluorometric) is an additional refinement of our earlier SuperSense™ international DNA methylation quantification kits by simplifying the workflow and enhancing the consistency of outcomes. It makes use of an progressive methodology to allow background alerts to be extraordinarily low, eliminating the plate drying and blocking steps. In comparison with chromatography-based strategies corresponding to HPLC or mass spectrometry, this product offers a cheap solution to precisely measure ranges of 5-methylcytosine. The equipment has the next benefits and options:

  • Fluorometric assay with easy-to-follow steps for comfort and velocity. All the process might be completed inside four hours.
  • Progressive equipment composition permits background alerts to be extraordinarily low, which eliminates the necessity for plate blocking and permits the assay to be easy, correct, dependable, and constant.
  • Excessive sensitivity, of which the detection restrict might be as little as 50 pg of methylated DNA and accepts as little as 20 ng of enter genomic DNA
  • Optimized antibody and enhancer options enable excessive specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine throughout the indicated focus vary of the pattern DNA.
  • Common optimistic and unfavourable controls are included, that are appropriate for quantifying methylated DNA from any species.
  • Strip-well microplate format makes the assay versatile: guide or excessive throughput evaluation.

Precept & Process
The MethylFlash™ Methylated DNA Quantification Package (Fluorometric) comprises all reagents needed for the quantification of worldwide DNA methylation. On this assay, DNA is certain to strip wells which might be particularly handled to have a excessive DNA affinity. The methylated fraction of DNA is detected utilizing seize and detection antibodies after which quantified fluorometrically by studying the absorbance in a fluorescence microplate spectrophotometer at excitation 530 and emission 590 nm. The quantity of methylated DNA is proportional to the RFU (relative fluorescence models) measured, which might be calculated with the equipment’s included formulation for relative methylation standing of two totally different DNA samples or absolute quantification of 5-methylcytosine (5-mC) utilizing a typical curve.

Simple, Quick, and Versatile
All the fluorometric assay has easy-to-follow steps for comfort and velocity, which might be accomplished in four hours. The strip-well microplate format permits for a versatile assay in guide or excessive throughput evaluation. Common optimistic and unfavourable controls are included with the equipment for quantifying methylated DNA from any species corresponding to mammals, vegetation, fungi, micro organism, and viruses.

Secure and Handy
All of the wanted reagents, together with unfavourable controls and optimistic controls, for quantification of worldwide DNA methylation are conveniently packaged within the equipment. The direct fluorometric quantification of DNA samples replaces out of date or inferior strategies and eliminates the necessity for DNA digestion/denaturation, radioactivity, extraction, or chromatography.

Methylated DNA Quantification Kit
Methylated DNA Quantification Package

Extremely Delicate and Particular
The novel process and proprietary equipment compositions enable for correct quantification of methylated DNA to be achieved with excessive sensitivity and specificity. The detection restrict of the enter DNA might be as little as 50 pg of methylated DNA.

MethylFlash (Fluorometric) Competitor Z Competitor C
Assay Precept Direct ELISA Direct ELISA Oblique ELISA
Format 96-well plate 96-well plate 96-well plate
Sensitivity Glorious detection restrict: 0.05 ng of 5-mC DNA Good detection restrict: >0.5 ng of 5-mC DNA Very poor detection restrict: >1000 ng of 5-mC DNA
S/N Ratio >20 with very low background  <four with excessive background  N/A*
Procedural Comfort No want for plate blocking and denaturation of DNA Requires plate blocking and DNA denaturation Requires plate blocking, DNA digestion, and antibody aggressive incubation in an extra plate
Protocol Time  <four hr  <four hr  >eight hr
DNA Kind & Species Common for any species, each ssDNA and dsDNA Human and mouse ssDNA solely Numerous species
Specificity Particular to 5-mC solely Cross-reaction to 5-hmC N/A*
Quantitation Kind Each relative and absolute quantification Relative Relative
Normal Management Steady, quantitative in absolute quantity of 5-mC, and might be universally used for any species Unstable, can’t be quantitative in quantity, and may solely be used for human or mouse N/A*
Minimal Enter Quantity 20 ng 100 ng 1000 ng
Accuracy of Detection Excessive, steady and quantified customary management. Confirmed shut correlation with LC-MS evaluation by customers Unclear, unstable and un-quantified customary management N/A*
% 5-mC calculation Easy Difficult Not Offered
Patented Technique Sure No No
Reputation Very excessive printed quotation depend Low printed quotation depend Low printed quotation depend
Help Experience Since 2006 Since 2013 Since 2011

 

Product Options
The MethylFlash™ Methylated DNA Quantification Package (Colorimetric) is an additional refinement of our earlier Methylamp™ international DNA methylation quantification kits by simplifying the workflow and enhancing the consistency of outcomes. It makes use of an progressive methodology to allow background alerts to be extraordinarily low, eliminating the plate drying and blocking steps. In comparison with chromatography-based strategies corresponding to HPLC or mass spectrometry, this product offers a cheap solution to precisely measure ranges of 5-methylcytosine. The equipment has the next benefits and options:

  • Colorimetric assay with easy-to-follow steps for comfort and velocity. All the process might be completed inside four hours.
  • Progressive equipment composition permits background alerts to be extraordinarily low, which eliminates the necessity for plate blocking and permits the assay to be easy, correct, dependable, and constant.
  • Excessive sensitivity, of which the detection restrict might be as little as 0.2 ng of methylated DNA and accepts as little as 50 ng of enter genomic DNA.
  • Optimized antibody and enhancer options enable excessive specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine throughout the indicated focus vary of the pattern DNA.
  • Common optimistic and unfavourable controls are included, that are appropriate for quantifying methylated DNA from any species.
  • Strip-well microplate format makes the assay versatile: guide or excessive throughput evaluation.

Methylamp MS-qPCR Fast Kit 

P-1028
  • EUR 595.92
  • EUR 213.40
  • EUR 380.60
  • 200 Reactions
  • 100 Reactions
  • 200 Reactions

Fast Eva QPCR SuperMix Kit

K5052002 200 rxn
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BrightGreen miRNA qPCR MasterMix-No Dye

MasterMix-mS 4 x 1.25 ml for 500 reactions
EUR 181.2

QuECHERS GC/MS Int Std

QUECR-MS-IS EACH
EUR 90.06

QuECHERS GC/MS QCSTD2

QUECR-MS-QC-2 EACH
EUR 189.24

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 253.2
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 384
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QuECHERS GC/MS QCSTD1 04

QUECR-MS-QC-1 EACH
EUR 182.4

QuECHERS GC/NPD and LC/MS

QUECR-NPD-MS-IS EACH
EUR 197.22

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
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PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

2X Universal SYBR Green Fast qPCR Mix

RK21203 1 mL Ask for price

Genious 2X SYBR Green Fast qPCR Mix

RK21204 1 mL Ask for price

AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2445.6
Description: Bioscience Mol Bio; Agarose

Methylamp DNA Modification Kit 

P-1001-1 40 Reactions
EUR 149.6

Methylamp DNA Modification Kit 

P-1001-2 80 Reactions
EUR 259.6

Methylamp DNA Modification Kit 

P-1001
  • EUR 416.70
  • EUR 149.60
  • EUR 259.60
  • 80 Reactions
  • 40 Reactions
  • 80 Reactions

Fast EvaGreen Master Mix for qPCR(200 rxn)

31003 2x1ML
EUR 244.8
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)

31003-1 5x1ML
EUR 463.2
Description: Minimum order quantity: 1 unit of 5x1ML

Fast EvaGreen Master Mix for qPCR (5000 rxn)

31003-2 50x1ML
EUR 3739.2
Description: Minimum order quantity: 1 unit of 50x1ML

Fast Plus EvaGreen qPCR Master Mix (200 rxn)

31020 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix (500 rxn)

31020-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)

31020-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX)

RK21206 1 ml Ask for price

Genious 2X SYBR GREEN FAST qPCR MIX (No ROX)

RK21205 1 ml Ask for price

Methylamp PCR Enhancer 

R-1002-1 400 Reactions
EUR 90.2

Methylamp PCR Enhancer 

R-1002
  • EUR 185.28
  • EUR 90.20
  • 400 Reactions
  • 400 Reactions

Methylamp 96 DNA Modification Kit 

P-1008-1 96 Samples
EUR 253

Methylamp Universal Methylated DNA Kit 

P-1011-1 10 Modifications
EUR 292.6

Methylamp Universal Methylated DNA Kit 

P-1011-2 20 Modifications
EUR 442.2

Methylamp RNA Bisulfite Conversion Kit  

P-9003-050 50 Reactions
EUR 213.4

Methylamp 96 DNA Modification Kit 

P-1008
  • EUR 416.70
  • EUR 253.00
  • 96 Samples
  • 96 Samples

Methylamp Universal Methylated DNA Kit 

P-1011
  • EUR 684.66
  • EUR 292.60
  • EUR 442.20
  • 20 Modifications
  • 10 Modifications
  • 20 Modifications