ELISA assay for quantitative and qualitative analysis of exosomes
Methods.
Miller, I. V., & Grunewald, T. G. (2015). Tumor-derived exosomes: Tiny envelopes for big stories. Biology of the Cell, 107 (9), 287-305.
Hong, C. S., Muller, L., Boyiadzis, M., & Whiteside, T. L. (2014). Isolation and characterization of CD34 + blast-derived exosomes in acute myeloid leukemia. PLoS One, 9 (8), e103310.
Tetta, C., Ghigo, E., Silengo, L., Deregibus, M. C., & Camussi, G. (2013). Extracellular vesicles as an emerging mechanism of communication between cells. Endocrine, 44 (1), 11-19.
Vishnubhatla, I., Corteling, R., Stevanato, L., Hicks, C., & Sinden, J. (2014). Development of stem cell-derived exosomes as cell-free regenerative medicine. J. Circ. Biomark, 143, 2.
Logozzi, M., De Milito, A., Lugini, L., Borghi, M., Calabrò, L., Spada, M., … & Fais, S. (2009). High levels of exosomes expressing CD63 and caveolin-1 in the plasma of patients with melanoma. (4), e5219.
Exo-TEST: ready to use ELISA quantification kits
ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions.
ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is then performed using appropriate detection antibodies against exosome-associated antigens, which can be either generic or cell / tissue specific exosomes. Lyophilized exosome standards characterized by protein content and particle number (NTA) allow the quantification of an unknown sample by a standard calibration curve.
Applications: Capture and quantification of exosomes from human biofluids and culture media. Exosome complex profiling. Preclinical research on non-invasive biomarkers for the detection and monitoring of a number of pathological conditions (inflammation, cancer, neurodegeneration, etc.).
Advantages: Ready to use, no initial purification of exosomes is required. User-friendly and suitable for the analysis of multiple markers. Available in TEST format (limited to 3 ELISA strips, 24 wells).
Reference:
Musante, L., Tataruch-Weinert, D., Kerjaschki, D., Henry, M., Meleady, P., & Holthofer, H. (2017). Residual extracellular urinary vesicles in ultracentrifugation supernatants after enrichment by dialysis by hydrostatic filtration. Journal of Extracellular Vesicles, 6 (1), 1267896.
Foster, B. P., Balassa, T., Benen, T. D., Dominovic, M., Elmadjian, G. K., Florova, V., … & Kyvelidou, C. (2016). Extracellular vesicles in the blood, milk and body fluids of the female and male urogenital tracts and with special regard to reproduction. Critical Reviews in Clinical Laboratory Sciences, (just accepted), 1-47.
Tas, F., Karabulut, S., Tilgen Yasasever, C., & Duranyildiz, D. (2015). Clinical significance of serum caveolin -1 levels in patients with melanoma. International Journal of Dermatology.
Zarovni, N., Corrado, A., Guazzi, P., Zocco, D., Lari, E., Radano, G., … & Chiesi, A. (2015). Integrated isolation and quantitative analysis of exosome-transmitted proteins and nucleic acids using immunocapsura approaches.
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