Computable Genomix qPCR

Bioinformatic GeneIndexer latent semantic indexing (LSI) plasmids

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Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine

Beforehand, we reported the detection of two novel bluetongue virus (BTV) strains (SPvvvv/02 and SPvvvv/03), presumably representing new BTV genotypes, in a batch of sheeppox vaccine. We developed type-specific RT-qPCR assays (concentrating on genome section 2) for these two new BTV strains. The restrict of detection of each assays was 10 genome copies/μl and no cross-reactivity with different BTV genotypes was noticed.
The efficiency of three different BTV group-specific diagnostic assays was additionally examined in opposition to the putative novel genotypes. RT-qPCR assays concentrating on BTV section 9 and 10 detected each strains (SPvvvv/02 and SPvvvv/03) whereas a BTV section 1 RT-qPCR assay was unable to detect both BTV pressure. The work offered right here expands upon the present repertoire of RT-qPCR assays for BTV genotype dedication.

A qPCR-based methodology for molecular subtype classification of urinary bladder most cancers – stromal gene expressions present greater prognostic values than intrinsic tumor genes

Transcriptome-based molecular subtypes of muscle-invasive bladder most cancers (MIBC) have been proven to be each prognostic and predictive, however are usually not utilized in routine medical follow.
We aimed to develop a possible, RT-qPCR-based methodology for molecular subtyping. First, we outlined a 68-gene set overlaying tumor intrinsic (luminal, basal, squamous, neuronal, epithelial-to-mesenchymal, in situ carcinoma) and stromal (immune, extracellular matrix, p53-like) signatures.
Then, classifier strategies with this 68-gene panel had been developed in silico and validated on public datasets with obtainable subtype class info (MDA, TCGA, Lund, Consensus). Lastly, expression of the chosen 68 genes was decided in 104 frozen tissue samples of our MIBC cohort by RT-qPCR utilizing the TaqMan Array Card platform and samples had been labeled by our newly developed classifiers.
The prognostic worth of every subtype classification system and molecular signature scores had been assessed. We discovered that the lowered marker set mixed with the developed classifiers had been in a position to reproduce the TCGA II, MDA, Lund and Consensus subtype classification techniques with an overlap of 79%, 76%, 69% and 64%, respectively. Importantly, we might efficiently classify 96% (100/104) of our MIBC samples through the use of RT-qPCR. Neuronal and luminal subtypes and low stromal gene expressions had been related to poor survival. In conclusion, we developed a strong and possible methodology for the molecular subtyping in line with the TCGA II, MDA, Lund and Consensus classifications. Our outcomes recommend that stromal signatures have a superior prognostic worth in comparison with tumor intrinsic signatures and subsequently underline the significance of tumor-stroma interplay through the development of MIBC.

Extreme-level Multiplexing in Digital PCR With Intercalating Dyes by Coupling Precise-Time Kinetics and Melting Curve Analysis

Digital polymerase chain response (dPCR) is a mature method that has enabled scientific breakthroughs in plenty of fields. Nonetheless, this know-how is primarily utilized in evaluation environments with high-level multiplexing representing a significant issue. Proper right here, we propose a novel method for multiplexing, generally known as amplification and melting curve analysis (AMCA), which leverages the kinetic knowledge in real-time amplification info and the thermodynamic melting profile using a reasonable intercalating dye (EvaGreen).

 

The technique trains a system comprised of supervised machine finding out fashions for proper classification, by benefit of the large amount of data from dPCR platforms. As a case analysis, we develop a model new 9-plex assay to detect mobilised colistin resistant (mcr) genes as clinically associated targets for antimicrobial resistance. Over 100,000 amplification events have been analysed, and for the optimistic reactions, the AMCA methodology experiences a classification accuracy of 99.33 ± 0.13%, an increase of 10.0% over using melting curve analysis. This work provides an cheap strategy of high-level multiplexing with out fluorescent probes, extending the benefits of dPCR in evaluation and scientific settings.

 

Detection of Helicobacter pylori in gastric most cancers tissue by way of histopathology, immunohistochemistry and real-time reverse transcription-PCR

 

Intention:Helicobacter pylori is usually detected based totally on hematoxylin-eosin (H-E) choices, nonetheless, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are further precise in chronic-gastritis. We evaluated the relevance of these checks in Peruvian gastric most cancers samples.

 

Provides & methods: We carried out and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides have been independently evaluated by three pathologists.

 

Outcomes:H. pylori was detected in 167/287 by way of H-E, 140/288 by way of IHC and 175/288 by way of RT-PCR, and positive-status have been associated (p < 0.001). H. pylori detection by H-E had a wonderful concordance with IHC (kappa index = 0.632) nonetheless poor with RT-PCR (kappa index = 0.317). Higher median gene-copies have been current in extreme H. pylori density by way of H-E or IHC (p < 0.001).

 

Conclusion: H-E evaluation is right in gastric most cancers, and IHC and RT-PCR can complement its outcomes.

 

Analytical validation of the droplet digital PCR assay for prognosis of spinal muscular atrophy

 

Background: Spinal muscular atrophy (SMA) is a progressive motor neuron sickness attributable to homozygote lack of exon 7 on the survival motor neuron 1 (SMN1) gene. The severity of the SMA phenotype is influenced by copies of SMN2, a gene that is extraordinarily homologous with SMN1.

 

Methods: We validated analytical effectivity of droplet digital polymerase chain response (ddPCR) for detection of copy amount variation of SMN1 and SMN2 genes for prognosis of SMA using scientific samples. For accuracy effectivity evaluation, ddPCR outcomes have been in distinction with these of multiplex ligation-dependent probe amplification (MLPA) as a reference regular. Copy numbers of SMN1/SMN2 exon 7 from 200 scientific samples have been concordant between ddPCR and MLPA.

 

Outcomes: For all samples, the copy number of SMN1/SMN2 exon 7 was concordant between MLPA and ddPCR. The ddPCR moreover confirmed acceptable ranges of repeatability and complete imprecision.

 

Conclusion: Because of this truth, ddPCR is predicted to be useful for SMA prognosis and to predict phenotypic severity of SMA victims by determining the copy amount ofSMN2in scientific laboratories.

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A multiplex PCR tools for the detection of three essential virulent genes in Enterococcus faecalis

A multiplex PCR tools that detects three essential virulence genes, gelE, hyl and asaI, in Enterococcus faecalis was developed. Analyses of the accessible sequences of the three essential virulence genes and the designed primers allowed us to develop the three-gene, multiplex PCR protocol that maintained the specificity of each primer pair. The following three amplicon bands for gelE, hyl and asaI have been even and distinct with product sizes of 213, 273 and 713 bp, respectively.

 

The multiplex PCR course of was validated with a whole of 243 E. faecalis strains that included 02 ATCC strains, 109 isolates from marine samples (sediment, water and sea meals), 22 isolates from cattle fodder, 79 isolates modern water samples and 31isolates from nosocomial samples. Specificity of the tools was indicated by amplification of solely three essential virulence genes gelE, hyl and asaI, and with none nonspecific bands. Exams for the limit of detection revealed that amplified genes from the sample with a minimal of 104 CFU/g or CFU/mL (10 cells/response) of E. faecalis and reduce cell load samples, after a Three h enrichment in NIOT-E. faecalis enrichment medium at 37 °C, a sensitivity diploma of 10 CFU/g or CFU/mL was achieved.

 

 

GPR119 Antibody

DF4892-200ul 200ul
EUR 350

GPR119 antibody

70R-31399 100 ug
EUR 294
Description: Rabbit polyclonal GPR119 antibody

GPR119 Antibody

1-CSB-PA007283
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR119. Recognizes GPR119 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/10000

GPR119 Antibody

R35841-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: G protein-coupled receptor 119

GPR119 Polyclonal Antibody

ABP53820-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240

GPR119 Polyclonal Antibody

ABP53820-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240

GPR119 Polyclonal Antibody

ABP53820-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240

GPR119 Polyclonal Antibody

40973 100ul
EUR 319

GPR119 Polyclonal Antibody

40973-100ul 100ul
EUR 302.4

GPR119 Polyclonal Antibody

40973-50ul 50ul
EUR 224.4

GPR119 Polyclonal Antibody

E040973 100μg/100μl
EUR 255
Description: Available in various conjugation types.

GPR119 Polyclonal Antibody

E20-71962 100ug
EUR 225
Description: Available in various conjugation types.

GPR119 Polyclonal Antibody

E44H04855 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR119 Polyclonal Antibody

E-AB-19928-120uL 120uL
EUR 240
Description: Unconjugated

GPR119 Polyclonal Antibody

E-AB-19928-200uL 200uL
EUR 399
Description: Unconjugated

GPR119 Polyclonal Antibody

E-AB-19928-20uL 20uL
EUR 73
Description: Unconjugated

GPR119 Polyclonal Antibody

E-AB-19928-60uL 60uL
EUR 143
Description: Unconjugated

OAEB01535-100UG - GPR119 Antibody

OAEB01535-100UG 100ug
EUR 349

OAAI00430-100UG - GPR119 Antibody

OAAI00430-100UG 100ug
EUR 299

OAAJ05768-100UL - GPR119 Antibody

OAAJ05768-100UL 100uL
EUR 319

GPR119 Rabbit Polyclonal Antibody

ES4819-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against GPR119 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR119 Rabbit Polyclonal Antibody

ES4819-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against GPR119 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR119 Rabbit Polyclonal Antibody

E10G13483 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR119 Rabbit Polyclonal Antibody

E10G06590 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR119 Polyclonal Conjugated Antibody

C40973 100ul
EUR 476.4

Polyclonal GPR119 Antibody (aa186-235)

APR16477G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (aa186-235). This antibody is tested and proven to work in the following applications:

Gpr119 rabbit polyclonal antibody, Serum

AP54974SU-N 200 µl Ask for price

Gpr119 rabbit polyclonal antibody, Serum

AP54975SU-N 200 µl Ask for price

Goat Polyclonal Antibody against GPR119

TA302500 100 µg Ask for price

Polyclonal GPR119 Antibody (Cytoplasmic Domain)

APR16478G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR119 Antibody (Cytoplasmic Domain)

APR16479G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications:

OASG03109-100UL - GPR119 Antibody - middle region

OASG03109-100UL 100ul
EUR 239

GPR119 rabbit polyclonal antibody, Aff - Purified

AP01207PU-N 100 µg Ask for price

GPR119 rabbit polyclonal antibody, Aff - Purified

SP4186P 50 µg Ask for price

G-Protein Coupled Receptor 119 (GPR119) Antibody

20-abx121407
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  • 100 ul
  • 200 ul
  • 30 ul

G-Protein Coupled Receptor 119 (GPR119) Antibody

abx215630-100ug 100 ug
EUR 526.8

G-Protein Coupled Receptor 119 (GPR119) Antibody

abx432763-200ul 200 ul
EUR 460.8

G-Protein Coupled Receptor 119 (GPR119) Antibody

abx432763-100g 100 µg
EUR 387.5

G Protein-Coupled Receptor 119 (GPR119) Antibody

20-abx324143
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  • Ask for price
  • 100 ug
  • 50 ug

G Protein-Coupled Receptor 119 (GPR119) Antibody

abx324143-100g 100 µg
EUR 250

G Protein-Coupled Receptor 119 (GPR119) Antibody

abx324143-50g 50 µg
EUR 187.5

GPR119 Rabbit Polyclonal (Cytoplasmic Domain) Antibody

TA317609 50 µg Ask for price

GPR119 Rabbit Polyclonal (Cytoplasmic Domain) Antibody

TA317659 50 µg Ask for price

GPR119

CSB-CL840575HU 10 μg plasmid + 200μl Glycerol Ask for price

Gpr119/ Rat Gpr119 ELISA Kit

ELI-08203r 96 Tests
EUR 1063.2

GPR119 siRNA

20-abx918416
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  • Ask for price
  • 15 nmol
  • 30 nmol

GPR119 siRNA

20-abx918417
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  • 15 nmol
  • 30 nmol

GPR119 siRNA

20-abx902267
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  • Ask for price
  • 15 nmol
  • 30 nmol

GPR119 Peptide

45-692P 0.1 mg
EUR 405.6
Description: GPR119 Peptide

GPR119 Rabbit pAb

A18544-100ul 100 ul
EUR 369.6

GPR119 Rabbit pAb

A18544-200ul 200 ul
EUR 550.8

GPR119 Rabbit pAb

A18544-20ul 20 ul
EUR 219.6

GPR119 Rabbit pAb

A18544-50ul 50 ul
EUR 267.6

GPR119 Rabbit pAb

E2864021 100ul
EUR 225
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR119 (untagged)-Human G protein-coupled receptor 119 (GPR119)

SC307189 10 µg Ask for price

GPR119 cloning plasmid

CSB-CL840575HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the GPR119 gene.

GPR119 Blocking Peptide

20-abx162463
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  • 1 mg
  • 5 mg

GPR119 Blocking Peptide

DF4892-BP 1mg
EUR 234

Gpr119 (untagged) - Mouse G-protein coupled receptor 119 (Gpr119), (10ug)

MC213024 10 µg Ask for price

GPR119 (GFP-tagged) - Human G protein-coupled receptor 119 (GPR119)

RG216685 10 µg Ask for price

Gpr119 (GFP-tagged) - Mouse G-protein coupled receptor 119 (Gpr119), (10ug)

MG224908 10 µg Ask for price

Gpr119 (Myc-DDK-tagged) - Mouse G-protein coupled receptor 119 (Gpr119)

MR224908 10 µg Ask for price

Mouse Gpr119 ELISA KIT

ELI-09750m 96 Tests
EUR 1038

Human GPR119 ELISA KIT

ELI-48829h 96 Tests
EUR 988.8

GPR119 (Myc-DDK-tagged)-Human G protein-coupled receptor 119 (GPR119)

RC216685 10 µg Ask for price

Rat GPR119 shRNA Plasmid

20-abx989158
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  • 150 µg
  • 300 µg

Mouse GPR119 shRNA Plasmid

20-abx982252
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  • Ask for price
  • 150 µg
  • 300 µg

Human GPR119 shRNA Plasmid

20-abx965265
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  • Ask for price
  • 150 µg
  • 300 µg

Recombinant Human GPR119 VLP

DAG-WT1519 100 µg, 1 mg Ask for price
Description: Recombinant

GPR119 Recombinant Protein (Rat)

RP203282 100 ug Ask for price

Anti- GPR162 Antibody Antibody

GWB-39B7E0 0.05 ml Ask for price

Anti- GPR162 Antibody Antibody

GWB-AAD3BE 0.05 ml Ask for price

Anti- GPR162 Antibody Antibody

GWB-C5010A 0.05 ml Ask for price

GPR119 Recombinant Protein (Human)

RP039541 100 ug Ask for price

GPR119 Recombinant Protein (Mouse)

RP139418 100 ug Ask for price

Gpr119 (untagged ORF) - Rat G protein-coupled receptor 119 (Gpr119), (10 ug)

RN205502 10 µg Ask for price

anti- GPR105 antibody

FNab03588 100µg
EUR 658.5
Description: Antibody raised against GPR105

anti- GPR107 antibody

FNab03589 100µg
EUR 702
Description: Antibody raised against GPR107

anti- GPR108 antibody

FNab03590 100µg
EUR 658.5
Description: Antibody raised against GPR108

anti- GPR116 antibody

FNab03591 100µg
EUR 658.5
Description: Antibody raised against GPR116

anti- GPR132 antibody

FNab03593 100µg
EUR 606.3
Description: Antibody raised against GPR132

anti- GPR137 antibody

FNab03594 100µg
EUR 606.3
Description: Antibody raised against GPR137

anti- GPR142 antibody

FNab03597 100µg
EUR 702
Description: Antibody raised against GPR142

anti- GPR161 antibody

FNab03598 100µg
EUR 606.3
Description: Antibody raised against GPR161

anti- GPR162 antibody

FNab03599 100µg
EUR 702
Description: Antibody raised against GPR162

anti- GPR177 antibody

FNab03601 100µg
EUR 658.5
Description: Antibody raised against GPR177

anti- GPR137B antibody

FNab03595 100µg
EUR 702
Description: Antibody raised against GPR137B

anti- GPR17 antibody

FNab03600 100µg
EUR 658.5
Description: Antibody raised against GPR17

anti- GPR22 antibody

FNab03603 100µg
EUR 606.3
Description: Antibody raised against GPR22

anti- GPR35 antibody

FNab03605 100µg
EUR 702
Description: Antibody raised against GPR35

anti- GPR50 antibody

FNab03607 100µg
EUR 658.5
Description: Antibody raised against GPR50

anti- GPR50 antibody

FNab03608 100µg
EUR 658.5
Description: Antibody raised against GPR50

anti- GPR81 antibody

FNab03610 100µg
EUR 702
Description: Antibody raised against GPR81

anti- GPR83 antibody

FNab03611 100µg
EUR 702
Description: Antibody raised against GPR83

Gpr119 ORF Vector (Rat) (pORF)

ORF067762 1.0 ug DNA
EUR 607.2
Christopher Miller

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