Category: Reagents
The MRD disk: automated minimal residual disease monitoring by highly sensitive centrifugal microfluidic multiplex qPCR
We current a proof-of-principle research on automated, extremely delicate and multiplexed qPCR quantification by centrifugal microfluidics. The MRD disk can be utilized for standardisation of repetitive, longitudinal assays with excessive necessities on reproducibility and sensitivity, equivalent to most cancers monitoring. In distinction to high-throughput qPCR automation by cumbersome and costly robotic workstations we make use…
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Roadblock-qPCR: A simple and inexpensive strategy for targeted measurements of mRNA stability
The steadiness of mRNAs is key to figuring out expression degree and dynamics. Nonetheless, present approaches for measuring transcript half-lives (e.g. transcription shutoff) are usually poisonous or technically advanced. Right here we describe an alternate technique for focused measurements of endogenous mRNA stability that’s easy, cheap, and non-toxic. Cells are first metabolically labelled with the…
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