computablegenomix

An optimized stepwise algorithm combining rapid antigen and RT-qPCR for screening of COVID-19 patients

Background & intention: We investigated the mixture of speedy antigen detection (RAD) and RT-qPCR assays in a stepwise process to optimize the detection of COVID-19. Strategies: From August 2020 to November 2020, 43,399 sufferers had been screened in our laboratory for COVID-19 diagnostic by RT-qPCR utilizing nasopharyngeal swab. General, 4,691 of the 43,399 had been discovered to…

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computablegenomix

maxRatio improves the detection of samples with abnormal amplification profiles on QIAgen’s artus HIV-1 qPCR assay

Background: Correct viral load (VL) willpower is paramount to find out the efficacy of anti-HIV-1 remedy. The standard methodology used, fit-point (FP), assumes an equal effectivity within the polymerase chain response (PCR) amongst samples which may not maintain for low-input templates. Another strategy, maxRatio, was launched to compensate for inhibition in PCR. Strategies: Herein, we assessed whether or…

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computablegenomix

A New Specific and Sensitive RT-qPCR Method Based on Splinted 5′ Ligation for the Quantitative Detection of RNA Species Shorter than microRNAs

Not too long ago, we found a brand new household of unusually brief RNAs mapping to five.8S ribosomal RNA (rRNA) and which we named dodecaRNAs (doRNAs), based on the variety of core nucleotides (12 nt) their members include. To verify these small RNA-sequencing (RNA-Seq) knowledge, validate the existence of the 2 overly ample doRNAs-the minimal…

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