Category: PCR
Selection and Validation of Reference Genes for RT-qPCR Analysis in Aegilops tauschii (Coss.) under Different Abiotic Stresses
Aegilops tauschii (Coss.) is an aggressive and severe annual grass weed in China. Its DD genome is a wealthy supply of genetic materials and performs higher beneath totally different abiotic stress circumstances (salinity, drought, temperature, and so on.). Reverse-transcribed quantitative polymerase chain response (RT-qPCR) is a dependable method for reference gene choice and validation. This work…
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Development of multiplex TaqMan qPCR for simultaneous detection and differentiation of eight common swine viral and bacterial pathogens
It’s laborious to diagnose the infections of classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus sort 2 (PCV2), and Suid herpesvirus 1 (SuHV-1) due to the same scientific signs in piglets. Staphylococcus aureus (S. aureus), Streptococcus suis (S. suis), Salmonella choleraesuis (S. choleraesuis, serotype: 6,7:c:1,5), and Escherichia coli (E.…
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Auto-qPCR; a python-based web app for automated and reproducible analysis of qPCR data
Quantifying modifications in DNA and RNA ranges is crucial in quite a few molecular biology protocols. Quantitative actual time PCR (qPCR) strategies have developed to grow to be commonplace, nonetheless, information evaluation contains many time-consuming and cumbersome steps, which might result in errors and misinterpretation of knowledge. To handle these bottlenecks, now we have developed…
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In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR
Quite a few RT-qPCR assays for the detection of SARS-CoV-2 have been printed and are listed by the WHO as advisable assays. Moreover, quite a few business assays with undisclosed primer and probe sequences are available on the market. Because the SARS-CoV-2 pandemic progresses, the virus accrues mutations, which in some circumstances – as seen…
Read MoreA novel multiplex qPCR method for assessing the comparative lengths of telomeres
Background: The comparative size of telomeres is taken into account to be associated to ailments corresponding to most cancers, growing older, and cardiovascular ailments. qPCR is presently one of many primary strategies for detecting telomere size. Nevertheless, because of the distinctive sequence of telomeres (extremely repetitive six-base sequence), it’s tough to design primers and probes to…
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Author Correction: A simple and robust real-time qPCR method for the detection of PIK3CA mutations
Background: Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has precipitated a pandemic of pneumonia spreading all over the world, resulting in critical threats to public well being and attracting monumental consideration. There’s an pressing want for delicate diagnostic testing implementation to regulate and handle SARS-CoV-2 in public well being laboratories. The quantitative reverse transcription PCR (RT-qPCR) assay is…
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Validation of fetal microchimerism after pregnancy in the ovine using qPCR
Fetal microchimerism has been detected in maternal tissues of people and rodents throughout and after being pregnant. Research specializing in fetal DNA switch to maternal tissues in home animals are restricted, particularly in sheep. Fetal ram DNA was noticed within the maternal circulation throughout being pregnant, however it isn’t recognized if this chimerism persists in…
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Ultra-sensitive AAV capsid detection by immunocapture-based qPCR following factor VIII gene transfer
Adeno-associated virus (AAV)-based gene remedy vectors are replication-incompetent and thus pose minimal threat for horizontal transmission or launch into the surroundings. In research with AAV5-FVIII-SQ (valoctocogene roxaparvovec), an investigational gene remedy for hemophilia A, residual vector DNA was detectable in blood, secreta, and excreta, nevertheless it remained unclear how lengthy structurally intact AAV5 vector capsids…
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RT-qPCR Diagnostics: The “Drosten” SARS-CoV-2 Assay Paradigm
The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an established tool for the diagnosis of RNA pathogens. Its potential for automation has caused it to be used as a presence/absence diagnostic tool even when RNA quantification is not required. This technology has been pushed to the forefront of public awareness by the COVID-19 pandemic,…
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A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
White lupin (Lupinus albus) represents an vital legume crop in Europe and different elements of the world on account of its excessive protein content material and potential for low-input agriculture. Nonetheless, most cultivars are vulnerable to anthracnose attributable to Colletotrichum lupini, a seed- and air-borne fungal pathogen that causes extreme yield losses. The goal of this work…
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