Category: PCR
The Discovery and Function of Filaggrin
Keratohyalin granules were discovered in the mid-19th century in cells that terminally differentiate to form the outer, cornified layer of the epidermis. The first indications of the composition of these structures emerged in the 1960s from a histochemical stain for histidine, followed by radioautographic evidence of a high incidence of histidine incorporation into newly synthesized…
Read MoreLabel-free real-time detection of biotinylated bovine serum albumin using a low-cost optical cavity-based biosensor.
We have developed a low-cost optical cavity-based biosensor with a differential detection method for point-of-care medical diagnostics. To experimentally demonstrate its label-free real-time biosensing capability, we performed the detection of biotinylated bovine serum albumin (BSA). Streptavidin is introduced into the optical cavity structure and immobilized on 3-aminopropyltriethoxysilane (APTES) coated surface. After rinsing out unbound streptavidin…
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pureexo exosome isolation kit
Further Data Product Tags Cat. No. BL-P100 PureExo Cell Producer 101Bio Key Options Straightforward to make use of: No ultra-centrifugation (< 2 hours) 10 fold greater yield (vs. different kits and ultracentrifuge) Save value (vs. antibodies-beads methodology) Isolate Pure exosome (>95%) Intact exosome (good morphology) Use as little as 2 mL cell media to realize…
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Cytochrome C ELISA Kits from MyBioSource
The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. Target-specific ELISA kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. Listed are ELISA Kits for the detection of cytochrome C, an alias name of cytochrome c, somatic. The…
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epigentek methylflash methylated dna quantification kit
Product Overview The MethylFlash Methylated DNA Quantification Package (Fluorometric) is a whole set of optimized buffers and reagents to fluorometrically quantify international DNA methylation by particularly measuring ranges of 5-methylcytosine (5-mC) in a microplate-based format. In comparison with LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash™ expertise straight quantifies precise international DNA methylation. This equipment can also be particularly optimized for…
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Current Practices for Reference Gene Selection in RT-qPCR of Aspergillus: Outlook and Recommendations for the Future
Aspergillus is a genus of filamentous fungi with huge geographic and ecological distributions. Species inside this genus are clinically, agriculturally and biotechnologically related, resulting in growing curiosity in elucidating gene expression dynamics of key metabolic and physiological processes. Reverse-transcription quantitative Polymerase Chain Response (RT-qPCR) is a delicate and particular methodology of quantifying gene expression. A vital…
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Direct RT-qPCR assay for SARS-CoV-2 variants of concern (Alpha, B.1.1.7 and Beta, B.1.351) detection and quantification in wastewater
Lower than a 12 months following the Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak, variants of concern have emerged within the type of variant Alpha (B.1.1.7, the British variant) and Beta (B.1.351, the South Africa variant). Resulting from their excessive infectivity and morbidity, it has grow to be clear that it’s essential to rapidly…
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Comparison of SARS-CoV-2 Detection by Rapid Antigen and by Three Commercial RT-qPCR Tests: A Study from Martin University Hospital in Slovakia
The worldwide pandemic of coronavirus illness 2019 (COVID-19) brought on by the extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is having an incredible affect on the worldwide financial system, well being care programs and the lives of virtually all folks on this planet. The Central European nation of Slovakia reached one of many highest day…
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A novel multiplex qPCR method for assessing the comparative lengths of telomeres
Background: The comparative size of telomeres is taken into account to be associated to illnesses similar to most cancers, growing old, and cardiovascular illnesses. qPCR is at the moment one of many fundamental strategies for detecting telomere size. Nevertheless, because of the distinctive sequence of telomeres (extremely repetitive six-base sequence), it’s troublesome to design primers and…
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Normalization of SARS-CoV-2 viral load via RT-qPCR provides higher-resolution data for comparison across time and between patients
The 2020 pandemic has reworked the world and elicited hundreds of research to raised perceive the SARS-CoV-2 virus. Viral load has been a typical measure to observe remedy therapies and affiliate viral dynamics with affected person outcomes; nevertheless, strategies related to viral load have diverse throughout research. These variations have the potential to sacrifice the…
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