Identification and three-dimensional reconstruction of oxytocin receptor expressing astrocytes in the rat and mouse brain

Right here, we current a step-by-step protocol for three-dimensional reconstruction of astrocyte morphology, utilized to the central amygdala oxytocin receptor-expressing astrocytes. This contains RNAse-free perfusion, mixture of RNAscope and immunohistochemistry, and confocal imaging. This protocol supplies detailed details about tissue dealing with and a complete description of the RNAScope approach to label rat and mouse…

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Sensitive, Signal-Modulation Strategy for Discrimination of ECL Spectra and Investigation of Mutual Interactions of Emitters

The depth of electrochemiluminescence (ECL) often modifications quickly with the progress of the electrochemical course of, making it tough to find out the ECL spectrum with a traditional photomultiplier in a wavelength scan mannequin. Herein, a band-pass filter (BPF)-involved modulating technique is proposed to improve a traditional ECL analyzer to a extremely delicate ECL spectrometer…

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Label-free real-time detection of biotinylated bovine serum albumin using a low-cost optical cavity-based biosensor.

We have developed a low-cost optical cavity-based biosensor with a differential detection method for point-of-care medical diagnostics. To experimentally demonstrate its label-free real-time biosensing capability, we performed the detection of biotinylated bovine serum albumin (BSA). Streptavidin is introduced into the optical cavity structure and immobilized on 3-aminopropyltriethoxysilane (APTES) coated surface. After rinsing out unbound streptavidin…

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Ultrasonographic observation in combination with progesterone monitoring for detection of ovulation in Labrador Retrievers

Although it is well known that the ovulation occurs during a period of time after LH surge in dogs, there are few reports of observing the entire process of development, ovulation, and luteinization of each follicle. This study aimed to detect the ovulation kinetics by ultrasonography in combination with progesterone monitoring, and therefore identify the…

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Green synthesis of zinc oxide particles with apple-derived compounds and their application as catalysts in the transesterification of methyl benzoates

ZnO nanoparticles (ZnONPs) were successfully synthesized using bravo-de-esmolfe apple extract in aqueous medium at room temperature. ZnO microparticles, prepared with a pure apple phytochemical, quercetin (ZnOq), or without phytochemicals (ZnO) were studied for comparative purposes. The re-use of apple waste for highly efficient catalyst production, based on green synthetic routes, can be added to the…

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Co-occurrence of odor-causing dioxanes and dioxolanes with bis(2-chloro-1-methylethyl) ether in Huangpu River source water and fates in O 3-BAC process

In recent years, dioxanes and dioxolanes have been intermittently detected in water environment and have caused several offensive drinking water odor incidents worldwide. In this study, the co-occurrence of eight dioxanes, twelve dioxolanes and bis(2-chloro-1-methylethyl) ether was investigated in Huangpu River watershed to explore potential sources and contributions to septic/chemical odor. Totally 8 dioxanes and…

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pureexo exosome isolation kit

pureexo exosome isolation kit

Further Data Product Tags Cat. No. BL-P100 PureExo Cell Producer 101Bio Key Options Straightforward to make use of: No ultra-centrifugation (< 2 hours) 10 fold greater yield (vs. different kits and ultracentrifuge) Save value (vs. antibodies-beads methodology) Isolate Pure exosome (>95%) Intact exosome (good morphology) Use as little as 2 mL cell media to realize…

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mybiosource

Cytochrome C ELISA Kits from MyBioSource

The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. Target-specific ELISA kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. Listed are ELISA Kits for the detection of cytochrome C, an alias name of cytochrome c, somatic. The…

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computablegenomix

epigentek methylflash methylated dna quantification kit

Product Overview The MethylFlash Methylated DNA Quantification Package (Fluorometric) is a whole set of optimized buffers and reagents to fluorometrically quantify international DNA methylation by particularly measuring ranges of 5-methylcytosine (5-mC) in a microplate-based format. In comparison with LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash™ expertise straight quantifies precise international DNA methylation. This equipment can also be particularly optimized for…

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computablegenomix

Chondrex: Arthritis and Inflammation Research Products

Chondrex, Inc. is happy to announce that we’re a brand new distributor for TdB Labs! TdB Labs has manufactured and supplied fluorescent and nonflurorescent dextran derivatives and different polysaccharides for the previous thirty years. Chondrex, Inc. is proud to distribute TdB Labs’s top quality and dependable merchandise alongside our personal merchandise worldwide. Chondrex, Inc. is happy to announce…

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