
An optimized stepwise algorithm combining rapid antigen and RT-qPCR for screening of COVID-19 patients
On the lookout for extra dependable biomarkers in breast most cancers: Comparability between routine strategies and RT-qPCR

Utility of Repeat Nasopharyngeal SARS-CoV-2 RT-PCR Testing and Refinement of Diagnostic Stewardship Strategies at a Tertiary Care Tutorial Center in a Low-Prevalence House of america
Background: Quite a few parts have led to a very extreme amount of maximum acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription polymerase chain response (RT-PCR) testing. Concerns exist about sensitivity and false-negative SARS-CoV-2 RT-PCR testing outcomes. We describe a retrospective observational analysis inspecting the utility of repeat nasopharyngeal (NP) SARS-CoV-2 RT-PCR testing at a tutorial coronary heart in a low-prevalence setting.
Methods: All victims inside our nicely being system with >1 NP SARS-CoV-2 RT-PCR examine end result have been included. SARS-CoV-2 RT-PCR testing was carried out based mostly on 1 of 4 validated assays. Key scientific and demographic info have been collected, along with whether or not or not the affected individual was inpatient or outpatient at time of the examine and whether or not or not the examine was carried out as part of a person under investigation (PUI) for potential coronavirus sickness 2019 or for asymptomatic screening.
Outcomes: An entire of 660 victims had >1 NP SARS-CoV-2 PCR examine carried out. The preliminary examine was unfavourable in 638. There have been solely 6 negative-to-positive conversions (0.9%). All 6 have been outpatients current course of a PUI workup 5-17 days after an preliminary unfavourable end result. In >260 inpatients with repeat testing, we found no instances of negative-to-positive conversion along with these current course of PUI or asymptomatic evaluation.
Conclusions: In a low-prevalence house, repeat inpatient testing after an preliminary unfavourable end result, using a extraordinarily analytically delicate SARS-CoV-2 RT-PCR, did not present negative-to-positive conversion. The scientific sensitivity of NP RT-PCR testing may be larger than beforehand believed. These outcomes have helped kind diagnostic stewardship pointers, significantly steering to decrease repeated testing inside the inpatient setting to optimize examine utilization and defend belongings.
Enchancment of a real-time PCR assay for detection and quantification of Streptococcus iniae using the lactate permease gene
The aim of this analysis is the occasion and evaluation of a quick and proper quantitative PCR (qPCR)-based protocol for detection of zoonotic pathogen Streptococcus iniae in bacterial cultures and tissues of diseased fish. For this purpose, the lactate permease-encoding (lldY) gene was chosen as a purpose for the design of S. iniae-specific primers based on comparative genomic analysis using 45 sequences retrieved from NCBI genome database. Specificity and applicability of these primers have been examined using 115 bacterial strains and fish tissues contaminated with S. iniae.
Sensitivity, reproducibility and effectivity of qPCR assay have been moreover determined. The developed qPCR assay confirmed 100% specificity with pure bacterial cultures or DNA extracted from S. iniae or tissues of fish contaminated with the bacterium. The technique has extreme sensitivity with a detection limit of 1.12 × 101 amplicon copies per assay (equal to 2 × 10-9 ng/µl) using bacterial DNA and of 1.44 × 101 gene copies in tissues of fish contaminated with S. iniae. In conclusion, this qPCR protocol provides an appropriate and delicate varied for the identification of S. iniae and its detection on fish tissues which may be carried out as a routine instrument in microbiological laboratories.
Enchancment of a New Multiplex Precise Time RT-PCR Assay for SARS-CoV-2 Detection
We describe the occasion of a model new multiplex precise time reverse transcription (RT)-PCR examine for detection of SARS-CoV-2, with primers designed to amplify a 108 bp purpose on the spike ground glycoprotein (S gene) and a hydrolysis Taqman probe designed to significantly detect SARS-CoV-2. We then evaluated the limit of detection (LOD) and scientific effectivity of this new assay. A LOD analysis with inactivated virus exhibited equal effectivity to the modified CDC assay with a closing LOD of 1,301 ± 13 genome equivalents/ml for the Northwell Nicely being Laboratories laboratory developed examine (NWHL LDT) vs. 1,249 ± 14 genome equivalents/ml for the modified CDC assay.
Furthermore, a scientific evaluation with 270 nasopharyngeal (NP) swab specimens exhibited 98.5% optimistic % settlement and 99.3% unfavourable % settlement compared with the modified CDC assay. The NWHL LDT multiplex design permits testing of 91 victims per plate, versus a most of 29 victims per plate on the modified CDC assay, providing the benefit of testing significantly further victims per run and saving reagents, all through a time when every of these parameters are essential.
The outcomes present that the NWHL LDT multiplex assay performs along with the modified CDC assay, nonetheless is further setting pleasant and worth environment friendly and could be utilized as a diagnostic assay and for epidemiological surveillance and scientific administration of SARS-CoV-2.
![]() Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
![]() GPR160 antibody |
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70R-50939 | Fitzgerald | 100 ul | EUR 292.8 |
Description: Purified Polyclonal GPR160 antibody |
![]() GPR160 antibody |
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70R-31407 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal GPR160 antibody |
![]() GPR160 Antibody |
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47605-100ul | SAB | 100ul | EUR 302.4 |
![]() GPR160 antibody |
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20R-GR067 | Fitzgerald | 50 ug | EUR 787.2 |
Description: Rabbit polyclonal GPR160 antibody |
![]() GPR160 Antibody |
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ABD4899 | Lifescience Market | 100 ug | EUR 525.6 |
![]() GPR160 Antibody |
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DF4899 | Affbiotech | 200ul | EUR 420 |
![]() GPR160 Antibody |
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1-CSB-PA008825 | Cusabio |
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Description: A polyclonal antibody against GPR160. Recognizes GPR160 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000 |
![]() Gpr160/ Rat Gpr160 ELISA Kit |
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ELI-27265r | Lifescience Market | 96 Tests | EUR 1063.2 |
![]() GPR160 Polyclonal Antibody |
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ABP54526-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350 |
![]() GPR160 Polyclonal Antibody |
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ABP54526-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350 |
![]() GPR160 Polyclonal Antibody |
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ABP54526-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of GPR160 from Human. This GPR160 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR160 at AA rangle: 270-350 |
![]() GPR160 Conjugated Antibody |
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C47605 | SAB | 100ul | EUR 476.4 |
![]() GPR160 Polyclonal Antibody |
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ES5525-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against GPR160 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
![]() GPR160 Polyclonal Antibody |
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ES5525-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against GPR160 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
![]() GPR160 siRNA |
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20-abx902275 | Abbexa |
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![]() GPR160 siRNA |
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20-abx918471 | Abbexa |
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![]() GPR160 siRNA |
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20-abx918472 | Abbexa |
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![]() Polyclonal GPR160 Antibody (Internal) |
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APR16513G | Leading Biology | 0.05ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 (Internal). This antibody is tested and proven to work in the following applications: |
![]() GPR160 Blocking Peptide |
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20-abx063814 | Abbexa |
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![]() GPR160 Blocking Peptide |
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DF4899-BP | Affbiotech | 1mg | EUR 234 |
![]() pCMV-SPORT6-GPR160 |
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PVT12413 | Lifescience Market | 2 ug | EUR 469.2 |
![]() Polyclonal GPR160 Antibody (Extracellular Domain) |
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APR16512G | Leading Biology | 0.05mg | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
![]() Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
![]() Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
![]() Polyclonal Goat anti-GST p-form |
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GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
![]() Human GPR160 shRNA Plasmid |
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20-abx958914 | Abbexa |
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![]() Mouse GPR160 shRNA Plasmid |
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20-abx977549 | Abbexa |
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![]() Rat GPR160 shRNA Plasmid |
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20-abx990910 | Abbexa |
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![]() GPR160 Recombinant Protein (Rat) |
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RP203351 | ABM | 100 ug | Ask for price |
![]() GPR160 Recombinant Protein (Human) |
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RP061095 | ABM | 100 ug | Ask for price |
![]() GPR160 Recombinant Protein (Mouse) |
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RP139520 | ABM | 100 ug | Ask for price |
![]() GPR160 Recombinant Protein (Mouse) |
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RP139523 | ABM | 100 ug | Ask for price |
![]() GPR160 Recombinant Protein (Mouse) |
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RP139526 | ABM | 100 ug | Ask for price |
![]() pECMV-3-FLAG-GPR160 |
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PVT12978 | Lifescience Market | 2 ug | EUR 843.6 |
![]() Polyclonal GPR160 Antibody - C-terminal region |
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APR16514G | Leading Biology | 0.05mg | EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR160 - C-terminal region. This antibody is tested and proven to work in the following applications: |
![]() G Protein-Coupled Receptor 160 (GPR160) Antibody |
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20-abx324785 | Abbexa |
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![]() Gpr160 ORF Vector (Rat) (pORF) |
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ORF067785 | ABM | 1.0 ug DNA | EUR 607.2 |
![]() GPR160 ORF Vector (Human) (pORF) |
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ORF020366 | ABM | 1.0 ug DNA | EUR 486 |
![]() Gpr160 ORF Vector (Mouse) (pORF) |
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ORF046508 | ABM | 1.0 ug DNA | EUR 607.2 |
![]() Gpr160 ORF Vector (Mouse) (pORF) |
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ORF046509 | ABM | 1.0 ug DNA | EUR 607.2 |
![]() Gpr160 ORF Vector (Mouse) (pORF) |
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ORF046510 | ABM | 1.0 ug DNA | EUR 607.2 |
![]() Probable G-Protein Coupled Receptor 160 (GPR160) Antibody |
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20-abx008198 | Abbexa |
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![]() Gpr160 sgRNA CRISPR Lentivector set (Rat) |
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K6604401 | ABM | 3 x 1.0 ug | EUR 406.8 |
![]() GPR160 sgRNA CRISPR Lentivector set (Human) |
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K0898801 | ABM | 3 x 1.0 ug | EUR 406.8 |
![]() Gpr160 sgRNA CRISPR Lentivector set (Mouse) |
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K4464401 | ABM | 3 x 1.0 ug | EUR 406.8 |
![]() Gpr160 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K6604402 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() Gpr160 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K6604403 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() Gpr160 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K6604404 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() GPR160 sgRNA CRISPR Lentivector (Human) (Target 1) |
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K0898802 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() GPR160 sgRNA CRISPR Lentivector (Human) (Target 2) |
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K0898803 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() GPR160 sgRNA CRISPR Lentivector (Human) (Target 3) |
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K0898804 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() Gpr160 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K4464402 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() Gpr160 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K4464403 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() Gpr160 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K4464404 | ABM | 1.0 ug DNA | EUR 184.8 |
![]() GPR160 Protein Vector (Human) (pPB-C-His) |
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PV081461 | ABM | 500 ng | EUR 662.4 |
![]() GPR160 Protein Vector (Human) (pPB-N-His) |
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PV081462 | ABM | 500 ng | EUR 662.4 |
![]() GPR160 Protein Vector (Human) (pPM-C-HA) |
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PV081463 | ABM | 500 ng | EUR 662.4 |
![]() GPR160 Protein Vector (Human) (pPM-C-His) |
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PV081464 | ABM | 500 ng | EUR 662.4 |
![]() GPR160 Protein Vector (Mouse) (pPB-C-His) |
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PV186030 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPB-N-His) |
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PV186031 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPM-C-HA) |
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PV186032 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPM-C-His) |
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PV186033 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPB-C-His) |
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PV186034 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPB-N-His) |
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PV186035 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPM-C-HA) |
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PV186036 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPM-C-His) |
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PV186037 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPB-C-His) |
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PV186038 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPB-N-His) |
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PV186039 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPM-C-HA) |
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PV186040 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Mouse) (pPM-C-His) |
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PV186041 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Rat) (pPB-C-His) |
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PV271138 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Rat) (pPB-N-His) |
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PV271139 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Rat) (pPM-C-HA) |
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PV271140 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 Protein Vector (Rat) (pPM-C-His) |
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PV271141 | ABM | 500 ng | EUR 723.6 |
![]() GPR160 3'UTR Luciferase Stable Cell Line |
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TU009240 | ABM | 1.0 ml | EUR 1672.8 |
![]() Gpr160 3'UTR GFP Stable Cell Line |
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TU255349 | ABM | 1.0 ml | Ask for price |
![]() GPR160 3'UTR GFP Stable Cell Line |
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TU059240 | ABM | 1.0 ml | EUR 1672.8 |
![]() Gpr160 3'UTR Luciferase Stable Cell Line |
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TU205349 | ABM | 1.0 ml | Ask for price |
![]() Gpr160 3'UTR Luciferase Stable Cell Line |
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TU108996 | ABM | 1.0 ml | Ask for price |
![]() Gpr160 3'UTR GFP Stable Cell Line |
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TU158996 | ABM | 1.0 ml | Ask for price |
![]() GPR160 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV683023 | ABM | 1.0 ug DNA | EUR 818.4 |
![]() GPR160 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV683027 | ABM | 1.0 ug DNA | EUR 818.4 |
![]() GPR160 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV683028 | ABM | 1.0 ug DNA | EUR 818.4 |
![]() Mouse Probable G- protein coupled receptor 160, Gpr160 ELISA KIT |
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ELI-48533m | Lifescience Market | 96 Tests | EUR 1038 |
![]() Gpr160 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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K6604405 | ABM | 3 x 1.0 ug | EUR 451.2 |
![]() GPR160 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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K0898805 | ABM | 3 x 1.0 ug | EUR 451.2 |
![]() Human Probable G- protein coupled receptor 160, GPR160 ELISA KIT |
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ELI-08752h | Lifescience Market | 96 Tests | EUR 988.8 |
![]() Gpr160 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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K4464405 | ABM | 3 x 1.0 ug | EUR 451.2 |
![]() GPR160 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV683024 | ABM | 1.0 ug DNA | EUR 818.4 |