Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine
A qPCR-based methodology for molecular subtype classification of urinary bladder most cancers – stromal gene expressions present greater prognostic values than intrinsic tumor genes
Extreme-level Multiplexing in Digital PCR With Intercalating Dyes by Coupling Precise-Time Kinetics and Melting Curve Analysis
Digital polymerase chain response (dPCR) is a mature method that has enabled scientific breakthroughs in plenty of fields. Nonetheless, this know-how is primarily utilized in evaluation environments with high-level multiplexing representing a significant issue. Proper right here, we propose a novel method for multiplexing, generally known as amplification and melting curve analysis (AMCA), which leverages the kinetic knowledge in real-time amplification info and the thermodynamic melting profile using a reasonable intercalating dye (EvaGreen).
The technique trains a system comprised of supervised machine finding out fashions for proper classification, by benefit of the large amount of data from dPCR platforms. As a case analysis, we develop a model new 9-plex assay to detect mobilised colistin resistant (mcr) genes as clinically associated targets for antimicrobial resistance. Over 100,000 amplification events have been analysed, and for the optimistic reactions, the AMCA methodology experiences a classification accuracy of 99.33 ± 0.13%, an increase of 10.0% over using melting curve analysis. This work provides an cheap strategy of high-level multiplexing with out fluorescent probes, extending the benefits of dPCR in evaluation and scientific settings.
Detection of Helicobacter pylori in gastric most cancers tissue by way of histopathology, immunohistochemistry and real-time reverse transcription-PCR
Intention:Helicobacter pylori is usually detected based totally on hematoxylin-eosin (H-E) choices, nonetheless, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are further precise in chronic-gastritis. We evaluated the relevance of these checks in Peruvian gastric most cancers samples.
Provides & methods: We carried out and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides have been independently evaluated by three pathologists.
Outcomes:H. pylori was detected in 167/287 by way of H-E, 140/288 by way of IHC and 175/288 by way of RT-PCR, and positive-status have been associated (p < 0.001). H. pylori detection by H-E had a wonderful concordance with IHC (kappa index = 0.632) nonetheless poor with RT-PCR (kappa index = 0.317). Higher median gene-copies have been current in extreme H. pylori density by way of H-E or IHC (p < 0.001).
Conclusion: H-E evaluation is right in gastric most cancers, and IHC and RT-PCR can complement its outcomes.
Analytical validation of the droplet digital PCR assay for prognosis of spinal muscular atrophy
Background: Spinal muscular atrophy (SMA) is a progressive motor neuron sickness attributable to homozygote lack of exon 7 on the survival motor neuron 1 (SMN1) gene. The severity of the SMA phenotype is influenced by copies of SMN2, a gene that is extraordinarily homologous with SMN1.
Methods: We validated analytical effectivity of droplet digital polymerase chain response (ddPCR) for detection of copy amount variation of SMN1 and SMN2 genes for prognosis of SMA using scientific samples. For accuracy effectivity evaluation, ddPCR outcomes have been in distinction with these of multiplex ligation-dependent probe amplification (MLPA) as a reference regular. Copy numbers of SMN1/SMN2 exon 7 from 200 scientific samples have been concordant between ddPCR and MLPA.
Outcomes: For all samples, the copy number of SMN1/SMN2 exon 7 was concordant between MLPA and ddPCR. The ddPCR moreover confirmed acceptable ranges of repeatability and complete imprecision.
Conclusion: Because of this truth, ddPCR is predicted to be useful for SMA prognosis and to predict phenotypic severity of SMA victims by determining the copy amount ofSMN2in scientific laboratories.
A multiplex PCR tools for the detection of three essential virulent genes in Enterococcus faecalis
A multiplex PCR tools that detects three essential virulence genes, gelE, hyl and asaI, in Enterococcus faecalis was developed. Analyses of the accessible sequences of the three essential virulence genes and the designed primers allowed us to develop the three-gene, multiplex PCR protocol that maintained the specificity of each primer pair. The following three amplicon bands for gelE, hyl and asaI have been even and distinct with product sizes of 213, 273 and 713 bp, respectively.
The multiplex PCR course of was validated with a whole of 243 E. faecalis strains that included 02 ATCC strains, 109 isolates from marine samples (sediment, water and sea meals), 22 isolates from cattle fodder, 79 isolates modern water samples and 31isolates from nosocomial samples. Specificity of the tools was indicated by amplification of solely three essential virulence genes gelE, hyl and asaI, and with none nonspecific bands. Exams for the limit of detection revealed that amplified genes from the sample with a minimal of 104 CFU/g or CFU/mL (10 cells/response) of E. faecalis and reduce cell load samples, after a Three h enrichment in NIOT-E. faecalis enrichment medium at 37 °C, a sensitivity diploma of 10 CFU/g or CFU/mL was achieved.
GPR119 Antibody | |||
DF4892-200ul | Affinity Biosciences | 200ul | EUR 350 |
GPR119 antibody | |||
70R-31399 | Fitzgerald | 100 ug | EUR 294 |
Description: Rabbit polyclonal GPR119 antibody |
GPR119 Antibody | |||
1-CSB-PA007283 | Cusabio |
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Description: A polyclonal antibody against GPR119. Recognizes GPR119 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/10000 |
GPR119 Antibody | |||
R35841-100UG | NSJ Bioreagents | 100 ug | EUR 339.15 |
Description: Additional name(s) for this target protein: G protein-coupled receptor 119 |
GPR119 Polyclonal Antibody | |||
ABP53820-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240 |
GPR119 Polyclonal Antibody | |||
ABP53820-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240 |
GPR119 Polyclonal Antibody | |||
ABP53820-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240 |
GPR119 Polyclonal Antibody | |||
40973 | SAB | 100ul | EUR 319 |
GPR119 Polyclonal Antibody | |||
40973-100ul | SAB | 100ul | EUR 302.4 |
GPR119 Polyclonal Antibody | |||
40973-50ul | SAB | 50ul | EUR 224.4 |
GPR119 Polyclonal Antibody | |||
E040973 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
GPR119 Polyclonal Antibody | |||
E20-71962 | EnoGene | 100ug | EUR 225 |
Description: Available in various conjugation types. |
GPR119 Polyclonal Antibody | |||
E44H04855 | EnoGene | 100ul | EUR 255 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GPR119 Polyclonal Antibody | |||
E-AB-19928-120uL | Elabscience Biotech | 120uL | EUR 240 |
Description: Unconjugated |
GPR119 Polyclonal Antibody | |||
E-AB-19928-200uL | Elabscience Biotech | 200uL | EUR 399 |
Description: Unconjugated |
GPR119 Polyclonal Antibody | |||
E-AB-19928-20uL | Elabscience Biotech | 20uL | EUR 73 |
Description: Unconjugated |
GPR119 Polyclonal Antibody | |||
E-AB-19928-60uL | Elabscience Biotech | 60uL | EUR 143 |
Description: Unconjugated |
OAEB01535-100UG - GPR119 Antibody | |||
OAEB01535-100UG | Aviva Systems Biology | 100ug | EUR 349 |
OAAI00430-100UG - GPR119 Antibody | |||
OAAI00430-100UG | Aviva Systems Biology | 100ug | EUR 299 |
OAAJ05768-100UL - GPR119 Antibody | |||
OAAJ05768-100UL | Aviva Systems Biology | 100uL | EUR 319 |
GPR119 Rabbit Polyclonal Antibody | |||
ES4819-100ul | ELK Biotech | 100ul | EUR 124 |
Description: A Rabbit Polyclonal antibody against GPR119 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
GPR119 Rabbit Polyclonal Antibody | |||
ES4819-50ul | ELK Biotech | 50ul | EUR 74 |
Description: A Rabbit Polyclonal antibody against GPR119 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
GPR119 Rabbit Polyclonal Antibody | |||
E10G13483 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GPR119 Rabbit Polyclonal Antibody | |||
E10G06590 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GPR119 Polyclonal Conjugated Antibody | |||
C40973 | SAB | 100ul | EUR 476.4 |
Polyclonal GPR119 Antibody (aa186-235) | |||
APR16477G | Leading Biology | 0.05ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (aa186-235). This antibody is tested and proven to work in the following applications: |
Gpr119 rabbit polyclonal antibody, Serum | |||
AP54974SU-N | Origene Technologies GmbH | 200 µl | Ask for price |
Gpr119 rabbit polyclonal antibody, Serum | |||
AP54975SU-N | Origene Technologies GmbH | 200 µl | Ask for price |
Goat Polyclonal Antibody against GPR119 | |||
TA302500 | Origene Technologies GmbH | 100 µg | Ask for price |
Polyclonal GPR119 Antibody (Cytoplasmic Domain) | |||
APR16478G | Leading Biology | 0.05mg | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications: |
Polyclonal GPR119 Antibody (Cytoplasmic Domain) | |||
APR16479G | Leading Biology | 0.05mg | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications: |
OASG03109-100UL - GPR119 Antibody - middle region | |||
OASG03109-100UL | Aviva Systems Biology | 100ul | EUR 239 |
GPR119 rabbit polyclonal antibody, Aff - Purified | |||
AP01207PU-N | Origene Technologies GmbH | 100 µg | Ask for price |
GPR119 rabbit polyclonal antibody, Aff - Purified | |||
SP4186P | Origene Technologies GmbH | 50 µg | Ask for price |
G-Protein Coupled Receptor 119 (GPR119) Antibody | |||
20-abx121407 | Abbexa |
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G-Protein Coupled Receptor 119 (GPR119) Antibody | |||
abx215630-100ug | Abbexa | 100 ug | EUR 526.8 |
G-Protein Coupled Receptor 119 (GPR119) Antibody | |||
abx432763-200ul | Abbexa | 200 ul | EUR 460.8 |
G-Protein Coupled Receptor 119 (GPR119) Antibody | |||
abx432763-100g | Abbexa | 100 µg | EUR 387.5 |
G Protein-Coupled Receptor 119 (GPR119) Antibody | |||
20-abx324143 | Abbexa |
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G Protein-Coupled Receptor 119 (GPR119) Antibody | |||
abx324143-100g | Abbexa | 100 µg | EUR 250 |
G Protein-Coupled Receptor 119 (GPR119) Antibody | |||
abx324143-50g | Abbexa | 50 µg | EUR 187.5 |
GPR119 Rabbit Polyclonal (Cytoplasmic Domain) Antibody | |||
TA317609 | Origene Technologies GmbH | 50 µg | Ask for price |
GPR119 Rabbit Polyclonal (Cytoplasmic Domain) Antibody | |||
TA317659 | Origene Technologies GmbH | 50 µg | Ask for price |
GPR119 | |||
CSB-CL840575HU | Cusabio | 10 μg plasmid + 200μl Glycerol | Ask for price |
Gpr119/ Rat Gpr119 ELISA Kit | |||
ELI-08203r | Lifescience Market | 96 Tests | EUR 1063.2 |
GPR119 siRNA | |||
20-abx918416 | Abbexa |
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GPR119 siRNA | |||
20-abx918417 | Abbexa |
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GPR119 siRNA | |||
20-abx902267 | Abbexa |
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GPR119 Peptide | |||
45-692P | ProSci | 0.1 mg | EUR 405.6 |
Description: GPR119 Peptide |
GPR119 Rabbit pAb | |||
A18544-100ul | Abclonal | 100 ul | EUR 369.6 |
GPR119 Rabbit pAb | |||
A18544-200ul | Abclonal | 200 ul | EUR 550.8 |
GPR119 Rabbit pAb | |||
A18544-20ul | Abclonal | 20 ul | EUR 219.6 |
GPR119 Rabbit pAb | |||
A18544-50ul | Abclonal | 50 ul | EUR 267.6 |
GPR119 Rabbit pAb | |||
E2864021 | EnoGene | 100ul | EUR 225 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GPR119 (untagged)-Human G protein-coupled receptor 119 (GPR119) | |||
SC307189 | Origene Technologies GmbH | 10 µg | Ask for price |
GPR119 cloning plasmid | |||
CSB-CL840575HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the GPR119 gene. |
GPR119 Blocking Peptide | |||
20-abx162463 | Abbexa |
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GPR119 Blocking Peptide | |||
DF4892-BP | Affbiotech | 1mg | EUR 234 |
Gpr119 (untagged) - Mouse G-protein coupled receptor 119 (Gpr119), (10ug) | |||
MC213024 | Origene Technologies GmbH | 10 µg | Ask for price |
GPR119 (GFP-tagged) - Human G protein-coupled receptor 119 (GPR119) | |||
RG216685 | Origene Technologies GmbH | 10 µg | Ask for price |
Gpr119 (GFP-tagged) - Mouse G-protein coupled receptor 119 (Gpr119), (10ug) | |||
MG224908 | Origene Technologies GmbH | 10 µg | Ask for price |
Gpr119 (Myc-DDK-tagged) - Mouse G-protein coupled receptor 119 (Gpr119) | |||
MR224908 | Origene Technologies GmbH | 10 µg | Ask for price |
Mouse Gpr119 ELISA KIT | |||
ELI-09750m | Lifescience Market | 96 Tests | EUR 1038 |
Human GPR119 ELISA KIT | |||
ELI-48829h | Lifescience Market | 96 Tests | EUR 988.8 |
GPR119 (Myc-DDK-tagged)-Human G protein-coupled receptor 119 (GPR119) | |||
RC216685 | Origene Technologies GmbH | 10 µg | Ask for price |
Rat GPR119 shRNA Plasmid | |||
20-abx989158 | Abbexa |
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Mouse GPR119 shRNA Plasmid | |||
20-abx982252 | Abbexa |
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Human GPR119 shRNA Plasmid | |||
20-abx965265 | Abbexa |
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Recombinant Human GPR119 VLP | |||
DAG-WT1519 | Creative Diagnostics | 100 µg, 1 mg | Ask for price |
Description: Recombinant |
GPR119 Recombinant Protein (Rat) | |||
RP203282 | ABM | 100 ug | Ask for price |
Anti- GPR162 Antibody Antibody | |||
GWB-39B7E0 | GenWay Biotech | 0.05 ml | Ask for price |
Anti- GPR162 Antibody Antibody | |||
GWB-AAD3BE | GenWay Biotech | 0.05 ml | Ask for price |
Anti- GPR162 Antibody Antibody | |||
GWB-C5010A | GenWay Biotech | 0.05 ml | Ask for price |
GPR119 Recombinant Protein (Human) | |||
RP039541 | ABM | 100 ug | Ask for price |
GPR119 Recombinant Protein (Mouse) | |||
RP139418 | ABM | 100 ug | Ask for price |
Gpr119 (untagged ORF) - Rat G protein-coupled receptor 119 (Gpr119), (10 ug) | |||
RN205502 | Origene Technologies GmbH | 10 µg | Ask for price |
anti- GPR105 antibody | |||
FNab03588 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against GPR105 |
anti- GPR107 antibody | |||
FNab03589 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against GPR107 |
anti- GPR108 antibody | |||
FNab03590 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against GPR108 |
anti- GPR116 antibody | |||
FNab03591 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against GPR116 |
anti- GPR132 antibody | |||
FNab03593 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against GPR132 |
anti- GPR137 antibody | |||
FNab03594 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against GPR137 |
anti- GPR142 antibody | |||
FNab03597 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against GPR142 |
anti- GPR161 antibody | |||
FNab03598 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against GPR161 |
anti- GPR162 antibody | |||
FNab03599 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against GPR162 |
anti- GPR177 antibody | |||
FNab03601 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against GPR177 |
anti- GPR137B antibody | |||
FNab03595 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against GPR137B |
anti- GPR17 antibody | |||
FNab03600 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against GPR17 |
anti- GPR22 antibody | |||
FNab03603 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against GPR22 |
anti- GPR35 antibody | |||
FNab03605 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against GPR35 |
anti- GPR50 antibody | |||
FNab03607 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against GPR50 |
anti- GPR50 antibody | |||
FNab03608 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against GPR50 |
anti- GPR81 antibody | |||
FNab03610 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against GPR81 |
anti- GPR83 antibody | |||
FNab03611 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against GPR83 |
Gpr119 ORF Vector (Rat) (pORF) | |||
ORF067762 | ABM | 1.0 ug DNA | EUR 607.2 |