Computable Genomix qPCR

Bioinformatic GeneIndexer latent semantic indexing (LSI) plasmids

computablegenomix
Ae1 Ae3 Antibody Antibodies Assay Kits Bafilomycin A1 Bile Acid Assay Biology Cells Canine Albumin cDNA Choline Acetyltransferase Antibody Clia Kits Culture Cells Deoxycholic Acid Sodium Salt Devices DNA DNA Templates DNA Testing Enzymes Equipments Exosomes F Actin Antibody Fto Antibody Gels Glycodeoxycholic Acid Gsk3 Alpha Hama Antibodies Hdac Activity Assay Isotypes Laminin Alpha 5 Mip 1B Mmp Activity Assay Mothers Against Decapentaplegic Mouse Albumin Elisa Panel Particles PCR Peptides Phospho 4Ebp1 Primary Antibodies Reagents Recombinant Proteins Ria Kits Secreted Alkaline Phosphatase Soft Agar Tgf Alpha Antibody Tgf Alpha Elisa Trypsin Activity Assay Tubastatin A Uric Acid Assay Valproic Acid Sodium Salt Vector & Virus

Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine

Beforehand, we reported the detection of two novel bluetongue virus (BTV) strains (SPvvvv/02 and SPvvvv/03), presumably representing new BTV genotypes, in a batch of sheeppox vaccine. We developed type-specific RT-qPCR assays (concentrating on genome section 2) for these two new BTV strains. The restrict of detection of each assays was 10 genome copies/μl and no cross-reactivity with different BTV genotypes was noticed.
The efficiency of three different BTV group-specific diagnostic assays was additionally examined in opposition to the putative novel genotypes. RT-qPCR assays concentrating on BTV section 9 and 10 detected each strains (SPvvvv/02 and SPvvvv/03) whereas a BTV section 1 RT-qPCR assay was unable to detect both BTV pressure. The work offered right here expands upon the present repertoire of RT-qPCR assays for BTV genotype dedication.

A qPCR-based methodology for molecular subtype classification of urinary bladder most cancers – stromal gene expressions present greater prognostic values than intrinsic tumor genes

Transcriptome-based molecular subtypes of muscle-invasive bladder most cancers (MIBC) have been proven to be each prognostic and predictive, however are usually not utilized in routine medical follow.
We aimed to develop a possible, RT-qPCR-based methodology for molecular subtyping. First, we outlined a 68-gene set overlaying tumor intrinsic (luminal, basal, squamous, neuronal, epithelial-to-mesenchymal, in situ carcinoma) and stromal (immune, extracellular matrix, p53-like) signatures.
Then, classifier strategies with this 68-gene panel had been developed in silico and validated on public datasets with obtainable subtype class info (MDA, TCGA, Lund, Consensus). Lastly, expression of the chosen 68 genes was decided in 104 frozen tissue samples of our MIBC cohort by RT-qPCR utilizing the TaqMan Array Card platform and samples had been labeled by our newly developed classifiers.
The prognostic worth of every subtype classification system and molecular signature scores had been assessed. We discovered that the lowered marker set mixed with the developed classifiers had been in a position to reproduce the TCGA II, MDA, Lund and Consensus subtype classification techniques with an overlap of 79%, 76%, 69% and 64%, respectively. Importantly, we might efficiently classify 96% (100/104) of our MIBC samples through the use of RT-qPCR. Neuronal and luminal subtypes and low stromal gene expressions had been related to poor survival. In conclusion, we developed a strong and possible methodology for the molecular subtyping in line with the TCGA II, MDA, Lund and Consensus classifications. Our outcomes recommend that stromal signatures have a superior prognostic worth in comparison with tumor intrinsic signatures and subsequently underline the significance of tumor-stroma interplay through the development of MIBC.

Extreme-level Multiplexing in Digital PCR With Intercalating Dyes by Coupling Precise-Time Kinetics and Melting Curve Analysis

Digital polymerase chain response (dPCR) is a mature method that has enabled scientific breakthroughs in plenty of fields. Nonetheless, this know-how is primarily utilized in evaluation environments with high-level multiplexing representing a significant issue. Proper right here, we propose a novel method for multiplexing, generally known as amplification and melting curve analysis (AMCA), which leverages the kinetic knowledge in real-time amplification info and the thermodynamic melting profile using a reasonable intercalating dye (EvaGreen).

 

The technique trains a system comprised of supervised machine finding out fashions for proper classification, by benefit of the large amount of data from dPCR platforms. As a case analysis, we develop a model new 9-plex assay to detect mobilised colistin resistant (mcr) genes as clinically associated targets for antimicrobial resistance. Over 100,000 amplification events have been analysed, and for the optimistic reactions, the AMCA methodology experiences a classification accuracy of 99.33 ± 0.13%, an increase of 10.0% over using melting curve analysis. This work provides an cheap strategy of high-level multiplexing with out fluorescent probes, extending the benefits of dPCR in evaluation and scientific settings.

 

Detection of Helicobacter pylori in gastric most cancers tissue by way of histopathology, immunohistochemistry and real-time reverse transcription-PCR

 

Intention:Helicobacter pylori is usually detected based totally on hematoxylin-eosin (H-E) choices, nonetheless, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are further precise in chronic-gastritis. We evaluated the relevance of these checks in Peruvian gastric most cancers samples.

 

Provides & methods: We carried out and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides have been independently evaluated by three pathologists.

 

Outcomes:H. pylori was detected in 167/287 by way of H-E, 140/288 by way of IHC and 175/288 by way of RT-PCR, and positive-status have been associated (p < 0.001). H. pylori detection by H-E had a wonderful concordance with IHC (kappa index = 0.632) nonetheless poor with RT-PCR (kappa index = 0.317). Higher median gene-copies have been current in extreme H. pylori density by way of H-E or IHC (p < 0.001).

 

Conclusion: H-E evaluation is right in gastric most cancers, and IHC and RT-PCR can complement its outcomes.

 

Analytical validation of the droplet digital PCR assay for prognosis of spinal muscular atrophy

 

Background: Spinal muscular atrophy (SMA) is a progressive motor neuron sickness attributable to homozygote lack of exon 7 on the survival motor neuron 1 (SMN1) gene. The severity of the SMA phenotype is influenced by copies of SMN2, a gene that is extraordinarily homologous with SMN1.

 

Methods: We validated analytical effectivity of droplet digital polymerase chain response (ddPCR) for detection of copy amount variation of SMN1 and SMN2 genes for prognosis of SMA using scientific samples. For accuracy effectivity evaluation, ddPCR outcomes have been in distinction with these of multiplex ligation-dependent probe amplification (MLPA) as a reference regular. Copy numbers of SMN1/SMN2 exon 7 from 200 scientific samples have been concordant between ddPCR and MLPA.

 

Outcomes: For all samples, the copy number of SMN1/SMN2 exon 7 was concordant between MLPA and ddPCR. The ddPCR moreover confirmed acceptable ranges of repeatability and complete imprecision.

 

Conclusion: Because of this truth, ddPCR is predicted to be useful for SMA prognosis and to predict phenotypic severity of SMA victims by determining the copy amount ofSMN2in scientific laboratories.

computablegenomix
computablegenomix

A multiplex PCR tools for the detection of three essential virulent genes in Enterococcus faecalis

A multiplex PCR tools that detects three essential virulence genes, gelE, hyl and asaI, in Enterococcus faecalis was developed. Analyses of the accessible sequences of the three essential virulence genes and the designed primers allowed us to develop the three-gene, multiplex PCR protocol that maintained the specificity of each primer pair. The following three amplicon bands for gelE, hyl and asaI have been even and distinct with product sizes of 213, 273 and 713 bp, respectively.

 

The multiplex PCR course of was validated with a whole of 243 E. faecalis strains that included 02 ATCC strains, 109 isolates from marine samples (sediment, water and sea meals), 22 isolates from cattle fodder, 79 isolates modern water samples and 31isolates from nosocomial samples. Specificity of the tools was indicated by amplification of solely three essential virulence genes gelE, hyl and asaI, and with none nonspecific bands. Exams for the limit of detection revealed that amplified genes from the sample with a minimal of 104 CFU/g or CFU/mL (10 cells/response) of E. faecalis and reduce cell load samples, after a Three h enrichment in NIOT-E. faecalis enrichment medium at 37 °C, a sensitivity diploma of 10 CFU/g or CFU/mL was achieved.

 

 

GPR119 Antibody

1-CSB-PA007283
  • Ask for price
  • Ask for price
  • 50ug
  • 100ug
Description: A polyclonal antibody against GPR119. Recognizes GPR119 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/10000

GPR119 Antibody

E19-4892 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GPR119 Antibody

E11-125G 100μg
EUR 225
Description: Available in various conjugation types.

GPR119 Antibody

G125-100ul 100μl
EUR 217
Description: GPR119 Rabbit Polyclonal Antibody

GPR119 Antibody

G125-50ul 50μl
EUR 143.5
Description: GPR119 Rabbit Polyclonal Antibody

GPR119 Antibody

DF4892 200ul
EUR 420

GPR119 Antibody

DF4892-100ul 100ul
EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

GPR119 Antibody

DF4892-200ul 200ul
EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

GPR119 Antibody

MBS9606344-01mL 0.1mL
EUR 260

GPR119 Antibody

MBS9606344-02mL 0.2mL
EUR 305

GPR119 Antibody

MBS9606344-5x02mL 5x0.2mL
EUR 1220

GPR119 Antibody

MBS8515594-01mg 0.1mg
EUR 305

GPR119 Antibody

MBS8515594-01mLAF405L 0.1mL(AF405L)
EUR 465

GPR119 Antibody

MBS8515594-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR119 Antibody

MBS8515594-01mLAF610 0.1mL(AF610)
EUR 465

GPR119 Antibody

MBS8515594-01mLAF635 0.1mL(AF635)
EUR 465

GPR119 Antibody

MBS851588-01mg 0.1mg
EUR 305

GPR119 Antibody

MBS851588-01mLAF405L 0.1mL(AF405L)
EUR 465

GPR119 Antibody

MBS851588-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR119 Antibody

MBS851588-01mLAF610 0.1mL(AF610)
EUR 465

GPR119 Antibody

MBS851588-01mLAF635 0.1mL(AF635)
EUR 465

GPR119 Antibody

MBS9507941-005mL 0.05mL
EUR 285

GPR119 Antibody

MBS9507941-01mL 0.1mL
EUR 385

GPR119 Antibody

MBS9507941-5x01mL 5x0.1mL
EUR 1590

Rabbit anti-Human GPR119 Polyclonal Antibody

MBS247454-005mg 0.05mg
EUR 615

Rabbit anti-Human GPR119 Polyclonal Antibody

MBS247454-5x005mg 5x0.05mg
EUR 2605

GPR119 Polyclonal Antibody

40973 100ul
EUR 319

GPR119 Polyclonal Antibody

40973-100ul 100ul
EUR 302.4

GPR119 Polyclonal Antibody

40973-50ul 50ul
EUR 224.4

GPR119 Polyclonal Antibody

ABP53820-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240

GPR119 Polyclonal Antibody

ABP53820-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240

GPR119 Polyclonal Antibody

ABP53820-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240

GPR119 Polyclonal Antibody

BT-AP03684-100ul 100ul Ask for price
Description: GPR119 encodes a member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The G protein-coupled receptor 119 is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.

GPR119 Polyclonal Antibody

BT-AP03684-20ul 20ul Ask for price
Description: GPR119 encodes a member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The G protein-coupled receptor 119 is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.

GPR119 Polyclonal Antibody

BT-AP03684-50ul 50ul Ask for price
Description: GPR119 encodes a member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The G protein-coupled receptor 119 is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.

GPR119 Polyclonal Antibody

E040973 100μg/100μl
EUR 255
Description: Available in various conjugation types.

GPR119 Polyclonal Antibody

E20-71962 100ug
EUR 225
Description: Available in various conjugation types.

GPR119 Polyclonal Antibody

E44H04855 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR119 Polyclonal Antibody

E-AB-19928-120uL 120uL
EUR 240
Description: Unconjugated

GPR119 Polyclonal Antibody

E-AB-19928-200uL 200uL
EUR 399
Description: Unconjugated

GPR119 Polyclonal Antibody

E-AB-19928-20uL 20uL
EUR 73
Description: Unconjugated

GPR119 Polyclonal Antibody

E-AB-19928-60uL 60uL
EUR 143
Description: Unconjugated

GPR119 Polyclonal Antibody

JOT-AP03684-100ul 100ul
EUR 220
Description: Human

GPR119 Polyclonal Antibody

JOT-AP03684-50ul 50ul
EUR 144
Description: Human

GPR119 Polyclonal Antibody

MBS2535409-002mL 0.02mL
EUR 140

GPR119 Polyclonal Antibody

MBS2535409-006mL 0.06mL
EUR 180

GPR119 Polyclonal Antibody

MBS2535409-012mL 0.12mL
EUR 260

GPR119 Polyclonal Antibody

MBS2535409-02mL 0.2mL
EUR 405

GPR119 Polyclonal Antibody

MBS2561817-002mL 0.02mL
EUR 135

GPR119 Polyclonal Antibody

MBS2561817-006mL 0.06mL
EUR 190

GPR119 Polyclonal Antibody

MBS2561817-012mL 0.12mL
EUR 265

GPR119 Polyclonal Antibody

MBS2561817-02mL 0.2mL
EUR 415

GPR119 Polyclonal Antibody

MBS2561817-5x02mL 5x0.2mL
EUR 1835

GPR119 Polyclonal Antibody

MBS9243808-01mL 0.1mL
EUR 415

GPR119 Polyclonal Antibody

MBS9243808-5x01mL 5x0.1mL
EUR 1841

GPR119 Polyclonal Antibody

MBS8522943-01mg 0.1mg
EUR 305

GPR119 Polyclonal Antibody

MBS8522943-01mLAF405L 0.1mL(AF405L)
EUR 465

GPR119 Polyclonal Antibody

MBS8522943-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR119 Polyclonal Antibody

MBS8522943-01mLAF610 0.1mL(AF610)
EUR 465

GPR119 Polyclonal Antibody

MBS8522943-01mLAF635 0.1mL(AF635)
EUR 465

GPR119 Polyclonal Antibody

MBS9413315-005mL 0.05mL
EUR 245

GPR119 Polyclonal Antibody

MBS9413315-01mL 0.1mL
EUR 305

GPR119 Polyclonal Antibody

MBS9413315-5x01mL 5x0.1mL
EUR 1230

GPR119 Polyclonal Antibody

RD219928A-120uL 120μL
EUR 360
Description: This gene encodes a member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The encoded protein is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.

GPR119 Polyclonal Antibody

RD219928A-200uL 200μL
EUR 630
Description: This gene encodes a member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The encoded protein is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.

GPR119 Polyclonal Antibody

RD219928A-20uL 20μL
EUR 109.5
Description: This gene encodes a member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The encoded protein is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.

GPR119 Polyclonal Antibody

RD219928A-60uL 60μL
EUR 214.5
Description: This gene encodes a member of the rhodopsin subfamily of G-protein-coupled receptors that is expressed in the pancreas and gastrointestinal tract. The encoded protein is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.

GPR119 Polyclonal Antibody

UB-GEN-13084 100 ul
EUR 200

OAAI00430-100UG - GPR119 Antibody

OAAI00430-100UG 100ug
EUR 299

OAAJ05768-100UL - GPR119 Antibody

OAAJ05768-100UL 100uL
EUR 319

OAEB01535-100UG - GPR119 Antibody

OAEB01535-100UG 100ug
EUR 349

GPR119 (V227) polyclonal antibody

BS2606 50ul
EUR 275
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

GPR119 Rabbit Polyclonal Antibody

E10G13483 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR119 Rabbit Polyclonal Antibody

E10G06590 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR119 Rabbit Polyclonal Antibody

ES4819-100ul 100ul
EUR 124
Description: WB, IF, ELISA

GPR119 Rabbit Polyclonal Antibody

ES4819-50ul 50ul
EUR 74
Description: WB, IF, ELISA

GPR119 Polyclonal Conjugated Antibody

C40973 100ul
EUR 476.4

GPR119 Polyclonal Conjugated Antibody

MBS9449820-01mLAF350 0.1mL(AF350)
EUR 480

GPR119 Polyclonal Conjugated Antibody

MBS9449820-01mLAF405 0.1mL(AF405)
EUR 480

GPR119 Polyclonal Conjugated Antibody

MBS9449820-01mLAF488 0.1mL(AF488)
EUR 480

GPR119 Polyclonal Conjugated Antibody

MBS9449820-01mLAF555 0.1mL(AF555)
EUR 480

GPR119 Polyclonal Conjugated Antibody

MBS9449820-01mLBiotin 0.1mL(Biotin)
EUR 480

Polyclonal GPR119 Antibody (aa186-235)

APR16477G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (aa186-235). This antibody is tested and proven to work in the following applications:

Gpr119 rabbit polyclonal antibody, Serum

AP54974SU-N 200 µl Ask for price

Gpr119 rabbit polyclonal antibody, Serum

AP54975SU-N 200 µl Ask for price

Goat Polyclonal Antibody against GPR119

TA302500 100 µg Ask for price

Polyclonal GPR119 Antibody (Cytoplasmic Domain)

APR16478G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR119 Antibody (Cytoplasmic Domain)

APR16479G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications:

OASG03109-100UL - GPR119 Antibody - middle region

OASG03109-100UL 100ul
EUR 239

GPR119 rabbit polyclonal antibody, Aff - Purified

AP01207PU-N 100 µg Ask for price

GPR119 rabbit polyclonal antibody, Aff - Purified

SP4186P 50 µg Ask for price

G-Protein Coupled Receptor 119 (GPR119) Antibody

abx432763-100g 100 µg
EUR 387.5

G-Protein Coupled Receptor 119 (GPR119) Antibody

abx432763-200ul 200 ul
EUR 460.8

G-Protein Coupled Receptor 119 (GPR119) Antibody

abx215630-100ug 100 ug
EUR 526.8

G-Protein Coupled Receptor 119 (GPR119) Antibody

20-abx121407
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  • 30 ul
  • 100 ul
  • 200 ul
Christopher Miller

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computablegenomix
computablegenomix